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A kind of microbial composite bacteria agent for ramie biological degumming pretreatment and preparation method thereof

A compound bacterial agent and biological degumming technology, applied in the field of biological degumming, can solve problems such as environmental pollution and water resource waste, achieve efficient decomposition, consume less energy and water, and achieve effective decomposition effects

Active Publication Date: 2021-12-17
FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention provides a microbial composite bacterial agent for ramie biological degumming pretreatment capable of large-scale industrial production and having a good decomposition effect on colloidal components, which solves the waste of water resources in the traditional chemical degumming process of the textile industry and the The difficult problem of polluting the environment; the present invention also provides the preparation method of this microbial composite bacterial agent, and preparation method is simple, and cost is lower, can realize ramie biological degumming effectively

Method used

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  • A kind of microbial composite bacteria agent for ramie biological degumming pretreatment and preparation method thereof
  • A kind of microbial composite bacteria agent for ramie biological degumming pretreatment and preparation method thereof
  • A kind of microbial composite bacteria agent for ramie biological degumming pretreatment and preparation method thereof

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Embodiment 1

[0027] Embodiment 1 of the present invention provides a kind of preparation method that is used for the microbial composite bacterial agent of ramie biological degumming pretreatment, comprises the following steps:

[0028] (1) Prepare a wort medium with a pH value of 7.0: weigh 30 g of yeast powder, 30 g of malt extract, 45 g of peptone, 115 g of glucose and 10 L of distilled water, mix them, and sterilize at 121° C. for 20 minutes;

[0029] (2) Prepare LB medium with a pH value of 7.0: weigh 100 g of peptone, 80 g of yeast extract, 75 g of sodium chloride and 10 L of distilled water, mix them, and sterilize at 121°C for 20 minutes;

[0030] (3), preparation pH value is 7.0 compound culture medium: take by weighing peptone 80g, yeast powder 40g, sodium chloride 55g, (NH 4 ) 2 SO 4 Mix 15g, 10g of dipotassium hydrogen phosphate, 70g of rice flour, 80g of raw ramie and 10L of distilled water, and sterilize at 121°C for 20min;

[0031] (4), preparation of microbial compound ...

Embodiment 2

[0045] Embodiment 2 of the present invention provides a kind of preparation method that is used for the microbial composite bacterial agent of ramie biological degumming pretreatment, comprises the following steps:

[0046] (1) Prepare a wort medium with a pH value of 7.2: weigh 25g of yeast powder, 25g of malt extract, 55g of peptone, 85g of glucose and 10L of distilled water, mix them, and sterilize at 121°C for 20 minutes;

[0047] (2) Prepare LB medium with a pH value of 7.2: weigh 120 g of peptone, 60 g of yeast extract, 65 g of sodium chloride and 10 L of distilled water, mix them, and sterilize at 121° C. for 20 minutes;

[0048] (3), preparation pH value is 7.2 compound culture medium: take by weighing peptone 65g, yeast powder 35g, sodium chloride 45g, (NH 4 ) 2 SO 4 Mix 18g, 8g of dipotassium hydrogen phosphate, 60g of rice flour, 70g of raw ramie and 10L of distilled water, and sterilize at 121°C for 20min;

[0049] (4), preparation of microbial compound bacteri...

Embodiment 3

[0057] Embodiment 3 of the present invention provides a kind of preparation method that is used for the microbial compound bacterial agent of ramie biological degumming pretreatment, comprises the following steps:

[0058] (1) Prepare a wort medium with a pH value of 7.0: weigh 35g of yeast powder, 35g of malt extract, 55g of peptone, 75g of glucose and 10L of distilled water, mix them, and sterilize at 121°C for 20 minutes;

[0059] (2) Prepare LB medium with a pH value of 7.0: weigh 135g of peptone, 90g of yeast extract, 95g of sodium chloride and 10L of distilled water, mix, and sterilize at 121°C for 20min;

[0060] (3), preparation pH value is 7.0 compound culture medium: take by weighing peptone 95g, yeast powder 45g, sodium chloride 65g, (NH 4 ) 2 SO 4 Mix 18g, 12g of dipotassium hydrogen phosphate, 85g of rice flour, 85g of raw ramie and 10L of distilled water, and sterilize at 121°C for 20 minutes;

[0061] (4), preparation of microbial compound bacteria agent: In...

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Abstract

The invention relates to the technical field of biological degumming, in particular to a microbial composite bacterial agent for ramie biological degumming pretreatment and a preparation method thereof. The preparation method comprises the following steps: respectively inoculating Bipedia, Rhizopus chrysporus, Erwinia, and Pantoea disperse into culture medium to obtain Bipedia bacterium liquid, Rhizopus chlamesporum liquid, Erwinia spp. Bacillus bacteria liquid and dispersed Pantoea bacteria liquid, these four kinds of bacterial liquids are mixed to obtain compound bacterial agent seed liquid; the compound bacterial agent seed liquid is inoculated into compound bacterial agent medium for mixed fermentation to obtain microbial compound bacteria agent. The preparation method provided by the invention is simple and low in cost, and can effectively realize biological degumming of ramie.

Description

technical field [0001] The invention relates to the technical field of biological degumming, in particular to a microbial composite bacterial agent for ramie biological degumming pretreatment and a preparation method thereof. Background technique [0002] Ramie (scientific name: Boehmeria nivea (L.) Gaudich.) is a subshrub or shrub plant of the genus Ramie of the family Urticaceae), its fibers have the characteristics of high strength and high modulus, and are widely used in industrial composite materials. China is the country with the largest ramie output, accounting for about 90% of the world's total output. The raw hemp of ramie contains 20-30% colloid, which broadly refers to non-cellulose components in raw hemp, including hemicellulose, pectin, lignin, water-soluble matter, fat wax, ash, etc. The degumming process in actual production is mainly to destroy the hemicellulose and pectin components to separate the fibers into single fibers to make them suitable for textile...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14D01C1/04C12R1/845C12R1/01C12R1/18C12R1/645
CPCC12N1/20C12N1/14D01C1/04
Inventor 王轶郭鹏包振华
Owner FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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