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High-temperature-resistant complex microbial inoculant capable of efficiently degrading wood fibers and application of high-temperature-resistant complex microbial inoculant

A technology of composite bacterial agent and wood fiber, applied in the field of agricultural microorganisms, can solve the problems of slow speed and insufficient degradation

Inactive Publication Date: 2020-10-23
TAICANG LVFENG BIOLOGICAL ORGANIC FERTILIZER CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the current problem of insufficient and slow degradation by a single strain of bacteria. Therefore, a microbial composite flora with the function of efficiently degrading straw is developed, specifically a high-temperature-resistant composite bacterial agent that efficiently degrades wood fiber and its application

Method used

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  • High-temperature-resistant complex microbial inoculant capable of efficiently degrading wood fibers and application of high-temperature-resistant complex microbial inoculant
  • High-temperature-resistant complex microbial inoculant capable of efficiently degrading wood fibers and application of high-temperature-resistant complex microbial inoculant
  • High-temperature-resistant complex microbial inoculant capable of efficiently degrading wood fibers and application of high-temperature-resistant complex microbial inoculant

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Embodiment Construction

[0018] Firstly, the original intention of the design of the present invention is explained, because the degradation of lignofiber by a single strain is insufficient and slow at present, so a microbial complex flora with the function of efficiently degrading straws has been developed, specifically a high-temperature-resistant degrading lignofiber Composite bacterial agent and its application, so as to replace the existing single bacterial species to explain, improve the degradation effect and degradation efficiency.

[0019] A method for preparing a high-temperature-resistant composite bacterial agent that efficiently degrades wood fibers, including NJAU-N30 and NJAU-N20 strains preserved in glycerin tubes at -80°C, activated by streaking on LB solid medium plates, and culturing in an incubator at 50°C for one hour; Then pick NJAU-N30 and NJAU-N20 single bacterium colony in 3ml liquid test tube 50 ℃, 170r / min shaking culture 10 hours, bacterium liquid is used as seed liquid; ℃,...

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Abstract

The invention discloses a high-temperature-resistant complex microbial inoculant capable of efficiently degrading wood fibers. The high-temperature-resistant complex microbial inoculant is prepared bymixing microbial composition fermentation broth in equal volume, the microbial composition comprises brevibacillus reuteri and bacillus, and the beneficial effects of the invention is as follows: thecomplex microbial inoculant comprises two single bacteria capable of efficiently degrading wood fibers, secretory enzymes of the single bacteria in the process of degrading the wood fibers have complementary functions and obvious synergistic effects, so that the wood fibers are quickly and fully degraded under a high-temperature condition, and thus the production efficiency of organic fertilizeris improved, and economic benefits and ecological environment benefits of enterprises are improved; and compared with single microbial inoculants, the complex microbial inoculants have more advantagesthat complex microbial inoculants can adapt to complex environments, and the effect is stronger and more complex, the degradation effect is better than that of a single bacterium or no bacterium, thedegradation time can be shortened, the degradation process can be accelerated, and the production cost can be saved.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a high-temperature-resistant composite bacterial agent for efficiently degrading wood fiber and its application. Background technique [0002] China is a big agricultural country in the world, and it is also a country with a lot of straw resources. After harvesting crops, more than 900 million tons of straw will be produced every year. In recent years, relevant departments at home and abroad have continuously explored the harmless treatment and resource utilization of crop straw, including direct returning to the field, technology of returning livestock to the field, straw power generation and ethanol production, etc. The crop straw cannot be effectively used and processed, and its processing procedure is complex, the degradation speed is slow, the cost is high, and it even causes more serious environmental pollution; therefore, how to quickly and effectively ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/00C05F11/08C05F17/20C12R1/07C12R1/01
CPCC05F11/00C05F11/08C12N1/20C05F17/20Y02W30/40
Inventor 李荣沈其荣陈伟施善巍何宙阳
Owner TAICANG LVFENG BIOLOGICAL ORGANIC FERTILIZER CO LTD
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