Toxicity detection method based on BOD difference before and after degradation of activated sludge

Abstract

The invention discloses a toxicity detection method based on BOD difference before and after degradation of activated sludge, which comprises the steps of firstly, preparing an activated sludge culture solution which comprises a nutrient solution, a trace element solution and a phosphoric acid buffer solution; culturing and domesticating the activated sludge for 5-6 days by utilizing the preparedactivated sludge culture solution to finish domestication of the activated sludge; mixing the domesticated activated sludge with a to-be-detected solution, degrading the to-be-detected solution by using the activated sludge, and measuring the COD difference before and after the reaction to represent the BOD inhibition rate; evaluating the biotoxicity of the to-be-detected solution by utilizing theBOD inhibition ratio, and judging the strength of the biotoxicity according to EC50. According to the method, the toxicity detection operation difficulty is simplified, the toxicity detection cost isreduced, the toxicity detection is normalized, and meanwhile, the purpose of BOD and toxicity integrated detection is also achieved.

Description

technical field [0001] The invention relates to the technical field of environmental toxicology, in particular to a toxicity detection method based on the BOD difference before and after activated sludge degradation. Background technique [0002] Toxic (such as heavy metal) pollution will not only threaten water quality safety, but also cause irreversible damage to human health. In order to ascertain the potential hazards of toxic substances in sewage, reduce the concentration of pollutants and achieve the discharge of wastewater up to standard, it is necessary to At present, the detection methods of heavy metals mainly include physical and chemical methods, biological toxicity detection methods and electrochemical biosensor methods, among which: [0003] Most of the physical and chemical methods rely on high-efficiency liquid chromatography, ultraviolet-visible spectrophotometer, and atomic absorption spectroscopy for detection. This method can realize the qualitative and ...

AI Technical Summary

Problems solved by technology

Electrochemical biosensor technology has the advantages of simplicity and high efficiency, but problems such as long start-up time, easy clogging of bioanodes, and energy degradation after long-term use cannot be ignored.

[0005] The above three schemes of the prior art all have defects. The first method has relatively high requirements for water samples and complicated pretreatment, and cannot explore the comprehensive toxicity of compounds in the water body; the second method takes a long time to cultivate and acclimatize the tested organisms and cannot reach The purpose of rapid detection of water bodies; although the third method has a shorter detection time, the electrode surface is easily blocked during the reaction process, which will affect the transfer of electrons. The electrical signal strength and stability of the electrochemical method need to be further improved. Therefore, it is urgent It is necessary to develop a new method for toxicity detection with simple operation, short detection time and low price

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