A small insect immobilization device for microinjection

Abstract

The invention relates to a small insect fixing device for microinjection, comprising a device base, a front clamping strip and a rear clamping strip arranged in the front and rear are arranged on the base of the device, and a plurality of front clamping strips are arranged at intervals along the left and right directions on the front clamping strip. Comb-tooth plate, a plurality of rear-bar comb-tooth plates are arranged at intervals along the left-right direction on the rear card-strip, each rear-bar-tooth-tooth plate is respectively arranged opposite to the corresponding front-bar-tooth-tooth plate, and two adjacent front card-tooth plates are respectively arranged opposite to each other. An insect fixing cavity is formed between the comb-tooth plate and the corresponding two adjacent rear-bar comb-tooth plates. The present invention provides a small insect immobilization device that restricts the action of insects during microinjection through a physical structure.

Description

technical field [0001] The invention relates to the technical field of small insect fixation, in particular to a small insect fixation device for microinjection. Background technique [0002] In the early research on RNAi, microinjection was mainly used in nematodes. With the progress of gene function research in Drosophila, it has become a method widely used in insects and can effectively introduce double-stranded RNA. , Coleoptera and other insects are widely used. [0003] During microinjection, in order to ensure the success rate of injection and the easy operation of injection, the movement behavior of small insects should be prohibited during the injection process. , the insects need to be anesthetized in advance, mainly including CO 2 Anesthesia, ether anesthesia, low temperature treatment, etc. However, there are various problems in the anesthesia process, such as CO 2 There is uncertainty in the time of anesthesia, which is easy to cause the death of insects due ...

AI Technical Summary

Problems solved by technology

[0003] When performing microinjection, in order to ensure the success rate of injection and the ease of injection, the movement behavior of small insects during the injection process should be prohibited. In the prior art, in order to avoid small insects moving around during the injection process , the insects need to be anesthetized in advance, mainly including CO 2 Anesthesia, ether anesthesia, low temperature treatment, etc. However, there are various problems in the anesthesia process, such as CO 2 There is uncertainty in the anesthesia time, which may easily lead to insect death due to over-anesthesia or insufficient anesthesia to move; ether anesthesia is likely to cause organic solvent pollution, which is harmful to the human body, and the dosage is difficult to grasp; The impact of insect limbs can cause problems with low injection success rates

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