Small insect fixing device for microinjection

A fixed device and microinjection technology, applied in veterinary instruments, animal restraint instruments, medical science, etc., can solve the problems of difficult to grasp the dosage, insect death, and low success rate of insect limb injection.

Active Publication Date: 2020-11-06
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] When performing microinjection, in order to ensure the success rate of injection and the ease of injection, the movement behavior of small insects during the injection process should be prohibited. In the prior art, in order to avoid small insects moving around during the injection process , the insects need to be anesthetized in advance, mainly including CO 2 Anesthesia, ether anesthesia, low temperature treatment, etc. However, there are various problems in the anesthesia process, such as CO 2 There is uncertainty in the anesthesia time, which may easily lead to insect death due to over-anesthesia or insufficient anesthesia to move; ether anesthesia is likely to cause organic solvent pollution, which is harmful to the human body, and the dosage is difficult to grasp; The impact of insect limbs can cause problems with low injection success rates

Method used

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  • Small insect fixing device for microinjection
  • Small insect fixing device for microinjection
  • Small insect fixing device for microinjection

Examples

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Embodiment Construction

[0027] An example of a small insect fixation device for microinjection is Figure 1~11 Shown: including the device base, the front clip 2 and the rear clip 3, the device base, the front clip and the rear clip are made of elastic transparent material, the transparent material is convenient for observation, and the elastic material can avoid damage to insects as much as possible. In this embodiment, the base of the device is assembled from an upper base part 9 and a lower base part 10. The specific assembly structure is that the bottom of the upper base part 9 is provided with two upper positioning columns 17 and two upper positioning columns. Positioning holes 18, the upper end of the lower base part 10 is provided with two corresponding lower positioning holes 15 and two lower positioning posts 16, the two upper positioning posts are in diagonal positions, and the two upper positioning holes In the diagonal position, when in use, each positioning column is positioned and plugg...

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Abstract

The invention relates to a small insect fixing device for microinjection. The small insect fixing device comprises a device base, wherein a front clamping strip and a rear clamping strip which are arranged front and back are arranged on the device base; a plurality of front clamping strip comb plates are arranged on the front clamping strip at intervals in the left-right direction; a plurality ofrear clamping strip comb plates are arranged on the rear clamping strip at intervals in the left-right direction; each rear clamping strip comb plate is opposite to the corresponding front clamping strip comb plate; and an insect fixing cavity is formed between every two adjacent front clamping strip comb plates and every two corresponding adjacent rear clamping strip comb plates. The invention provides the small insect fixing device for limiting the action of insects during microinjection through a physical structure.

Description

technical field [0001] The invention relates to the technical field of fixing small insects, in particular to a small insect fixing device for microinjection. Background technique [0002] In the early research on RNAi, microinjection was mainly used in nematodes. With the development of gene function research in Drosophila, it has become a method widely used in insects and can effectively introduce double-stranded RNA, and it is gradually used in Hymenoptera and Lepidoptera. , Coleoptera and other insects are widely used. [0003] When performing microinjection, in order to ensure the success rate of injection and the ease of injection, the movement behavior of small insects during the injection process should be prohibited. In the prior art, in order to avoid small insects moving around during the injection process , the insects need to be anesthetized in advance, mainly including CO 2 Anesthesia, ether anesthesia, low temperature treatment, etc. However, there are vario...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D3/00A61D7/00
CPCA61D3/00A61D7/00
Inventor 卢少华鲁玉杰吴宗霖张鑫鹏许一帆陈娟曹宇飞
Owner HENAN UNIVERSITY OF TECHNOLOGY
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