Method for oleophilic biodegradation of large amount of crude oil in soil and activator thereof
A biodegradation and activator technology, applied in the field of bioremediation of petroleum-contaminated soil, can solve the problems of low biological activity, inability to effectively degrade crude oil, slow growth rate, etc., and achieve the effect of improving biodegradation
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[0035] 1) Preparation of activator:
[0036] Mix the ammonium salt ammonium chloride, ammonium nitrate or ammonium sulfate, acetic acid, glucose and distilled water according to the mass ratio (1~4):(10~14):(3~6):(20~50) to get the activation agent;
[0037] Wherein, glucose is fructose, D-glucose or galactose.
[0038] 2) Add the activator, oily soil and distilled water prepared in step 1) into the container according to the mass ratio (2~5):(4~6):(50~70), mix them, and shake at 130~140r / min Oxygenation is carried out by oscillating once every 6 hours on the device, and the oleophilic biodegradation process of a large amount of crude oil in the soil is carried out at room temperature for 20 to 30 days.
[0039] Among them, the oil-bearing soil includes high-oil soil and low-oil soil. The petroleum hydrocarbon content in the high-oil soil is 13.8g / kg, and the petroleum hydrocarbon content in the low-oil soil is 8.5g / kg.
[0040] Next, the oilophilic biodegradation performan...
Embodiment 1
[0053] The oil used is high-oil soil, the ammonium salt is ammonium chloride, and the glucose is fructose.
[0054] 1) Preparation of activator:
[0055] Mix the ammonium salt ammonium chloride, acetic acid, fructose and distilled water in a mass ratio of 1:12:6:30 to obtain the activator;
[0056] 2) Add the activator, oil-containing soil and distilled water prepared in step 1) into the container according to the mass ratio of 3:5:60, mix them, place them on a 140r / min shaker for 6 hours to oscillate and oxygenate once, and carry out 20 minutes at room temperature. Oleophilic biodegradation process of bulk crude oil in soil.
[0057] After the reaction, the growth rate of petroleum bacteria was as high as 3.3log CFU / g soil, and the growth rate was as high as 48%, and the activity was also very high (0.8mgCO 2 / kg soil), a large amount of crude oil (C 18 ~C 30 ) removal amount is as high as 3313mg / kg, that is, the activator stimulates the rapid reproduction of highly activ...
Embodiment 2
[0059] The oil used is high-oil soil, the ammonium salt is ammonium nitrate, and the glucose is D-glucose.
[0060] 1) Preparation of activator:
[0061] Mix the ammonium salt ammonium nitrate, acetic acid, glucose and distilled water in a mass ratio of 2:11:5:40 to obtain the activator;
[0062] 2) Add the activator, oil-containing soil and distilled water prepared in step 1) into the container according to the mass ratio of 3:5:60, mix them, place them on a 135r / min shaker for 6 hours and oscillate once for 28 hours at room temperature. Oleophilic biodegradation process of bulk crude oil in soil.
[0063] After the reaction, the growth rate of petroleum bacteria was as high as 3.2log CFU / g soil, and the growth rate was as high as 46%, and the activity was also very high (0.75mgCO 2 / kg soil), a large amount of crude oil (C 18 -C 30 ) removal amount is as high as 3310mg / kg, that is, the activator stimulates the rapid reproduction of highly active oleophilic organisms, and...
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