Antibacterial peptide and application thereof in preparation of medicines or cosmetics

An antifungal drug and antibacterial peptide technology, applied in cosmetics, cosmetic preparations, antifungal agents, etc., can solve the problems of no research and no screening of antibacterial peptides, and achieve the effect of significant inhibitory activity and low toxicity

Active Publication Date: 2020-11-13
张雪山
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although there are many studies on antimicrobial peptides, antibacterial peptides that can

Method used

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  • Antibacterial peptide and application thereof in preparation of medicines or cosmetics
  • Antibacterial peptide and application thereof in preparation of medicines or cosmetics
  • Antibacterial peptide and application thereof in preparation of medicines or cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Screening of anti-Trichophyton rubrum bactericidal peptides

[0023] 1. Bacteria culture

[0024] The standard strain of Trichophyton rubrum ATCC®28188 was planted on Sabouraud dextrose agar SDA plates, cultured in the dark at 27°C, picked a single colony and put it into a shaker flask of 250mL PDA medium, 150r / min, cultured in the dark at 27°C, Then, centrifuge at 3000 r / min, 4°C for 10 min, and collect the bacterial cells to harvest the bacterial cells for later use.

[0025] 2. Lipid extraction

[0026] 8 g of the collected bacterial cells were washed repeatedly with PBS, centrifuged, resuspended in 60 mL Tris-HCl buffer (25 mmol / L, pH7.5), ultrasonically disrupted, and after centrifugation, 180 mL of chloroform-methanol (1:2 , v / v) mixed solvents, mixed and stirred for 100 min. After that, add 40 mL of chloroform and 40 mL of distilled water, and continue stirring for 30 min. The chloroform phase was separated with a separatory funnel, and the solvent ...

Embodiment 2

[0036] The mensuration of embodiment 2 antimicrobial peptide minimum inhibitory concentration

[0037] The minimum inhibitory concentrations (Minimalinhibitoryconcentrations, MICs) of antimicrobial peptides against indicator bacteria were determined by microdilution method.

[0038] The strains tested were: (1) Escherichia coli ATCC25922; (2) Trichophyton mentagrophytes, NRR00060 of Beijing Huayueyang Biotech Co., Ltd.; (3) Trichophyton rubrum ATCC®28188. The strains were cultured according to conventional culture methods. After culturing to the logarithmic phase, the bacterial cells in the logarithmic phase were collected by centrifugation (3000 r / min, 4°C, 10 min), washed with sterilized PBS, and resuspended until the OD600 was about 0.3. The peptide was prepared into a stock solution of 1024 µg / mL with sterilized PBS, sterilized by filtration with a 0.22 µm filter membrane, and serially diluted with sterilized PBS. Add 100 µL medium, 100 µL test bacteria solution and 50 µ...

experiment example 3

[0042] Experimental example 3 The effect of antimicrobial peptides in the treatment and prevention of skin ringworm

[0043] Trichophyton rubrum was activated and inoculated in SDA medium, cultured at 30°C for 7 days, eluted with sterile normal saline, shaken and mixed well, and adjusted the concentration of the mixed bacterial suspension to 1.0×10 8 CFU / mL. Anesthesia was injected intraperitoneally, and the back and sides of guinea pigs were sheared and depilated respectively to form a hairless area of ​​about 2.5×2.5 cm each. After the hairless area was polished with sterile sandpaper until spot-like bleeding, 100 μL of bacterial suspension was evenly applied. On the 3rd day of inoculating the bacterial solution, 25 guinea pigs successfully modeled were numbered respectively and divided into 5 groups according to the random number table method (negative control group / 5% ethanol solvent group / 8 μg / mL antimicrobial peptide / 32 μg / mL antimicrobial peptide / positive control gro...

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Abstract

The invention relates to an antibacterial peptide and an application thereof in preparation of medicines or cosmetics. The antibacterial peptide has significant inhibitory activity on human pathogenicfungi trichophyton rubrum, and the compound also has the advantage of low toxicity, so that the antibacterial peptide can be used for preparing antifungal drugs and cosmetics.

Description

technical field [0001] The invention belongs to the field of medicines, and in particular relates to an antibacterial peptide and its application in the preparation of medicines or cosmetics. Background technique [0002] Fungal skin disease, also known as dermatophytosis, is an infection caused by superficial fungi invading human skin, hair, and deck, referred to as ringworm. The pathogenic fungi of tinea manuum and tinea pedis are mainly Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum gypsumoid and Epidermophyton flocculus, of which Trichophyton rubrum accounts for more than 60%, and has become the current hand, The main pathogenic bacteria of tinea pedis. Trichophyton rubrum (Trichophy ton rubrum ) is a mitotic spore fungus and is the most common pathogenic dermatophyte in humans. It is distributed worldwide and is the most common dermatophyte in my country. Trichophyton rubrum can cause skin and hair infections; and often causes chronic, refractory infec...

Claims

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Application Information

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IPC IPC(8): C07K14/00A61K38/16A61P17/00A61P31/10A61K8/64A61Q19/10A61Q17/00
CPCA61K8/64A61K38/00A61Q17/005A61Q19/10A61P17/00A61P31/10C07K14/001
Inventor 张海涛沈伟
Owner 张雪山
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