New coronavirus RBD specific monoclonal antibody and application

A monoclonal antibody and specific technology, applied in the direction of antibodies, applications, antiviral agents, etc., can solve the problem of limited virus blocking effect, achieve the effect of improving specific binding ability and wide application value

Active Publication Date: 2020-11-13
CHONGQING MEDICAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The Chinese invention patent application with the application publication number CN111303280A discloses a fully human monoclonal antibody with high neutralizing activity against SARS-CoV-2. The above patent provides a fully human monoclonal antibody whose recognition region is the S1 non-RBD region. However, since the new coronavirus invades the host cell through RBD binding to ACE2 of the host cell, the fully human monoclonal antibody obtained in the above patent has limited blocking effect on the virus, and the above patent obtains antibody cDNA by labeling plasma cells, but Compared with plasma cells, memory B cells respond quickly after activation, so memory B cells can trigger a faster and stronger humoral immune response than the initial response, while plasma cells trigger a limited humoral immune response

Method used

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  • New coronavirus RBD specific monoclonal antibody and application
  • New coronavirus RBD specific monoclonal antibody and application
  • New coronavirus RBD specific monoclonal antibody and application

Examples

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Effect test

Embodiment 1

[0021] This embodiment provides a novel coronavirus RBD-specific monoclonal antibody, the amino acid sequence of the heavy chain is shown in SEQ ID NO:1; the amino acid sequence of the light chain is shown in SEQ ID NO:2.

[0022] This embodiment also provides the application of the above-mentioned novel coronavirus RBD-specific monoclonal antibody in the preparation of reagents or drugs for detecting or diagnosing SARS-CoV-2.

[0023] In actual production, this embodiment can be used to obtain a new coronavirus RBD-specific monoclonal antibody to prepare nucleic acid molecules, or to prepare expression cassettes, recombinant vectors, recombinant bacteria or transgenic cell lines containing the nucleic acid molecules, or to prepare pharmaceutical compositions. The pharmaceutical composition includes the above-mentioned novel coronavirus RBD-specific monoclonal antibody and pharmaceutically acceptable excipients, diluents or carriers.

[0024] In application, this embodiment ob...

Embodiment 2-20

[0026] The difference between Example 2-20 and Example 1 is that the amino acid sequence of the novel coronavirus RBD-specific monoclonal antibody is different, and the amino acid sequence of Example 2-20 is shown in the following table:

[0027] test group heavy chain amino acid sequence light chain amino acid sequence Example 1 - CQTS021 SEQ ID NO:1 SEQ ID NO:2 Example 2 - CQTS022 SEQ ID NO:3 SEQ ID NO:4 Example 3 - CQTS023 SEQ ID NO:5 SEQ ID NO:6 Embodiment 4-CQTS025 SEQ ID NO:7 SEQ ID NO:8 Example 5 - CQTS026 SEQ ID NO:9 SEQ ID NO:10 Embodiment 6-CQTS028 SEQ ID NO: 11 SEQ ID NO:12 Embodiment 7-CQTS029 SEQ ID NO: 13 SEQ ID NO: 14 Embodiment 8-CQTS030 SEQ ID NO: 15 SEQ ID NO: 16 Example 9 - CQTS031 SEQ ID NO: 17 SEQ ID NO: 18 Example 10 - CQTS032 SEQ ID NO: 19 SEQ ID NO: 20 Example 11 - CQTS033 SEQ ID NO: 21 SEQ ID NO: 22 Example 12 - CQTS034 SEQ ID NO: 23 S...

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Abstract

The invention belongs to the technical field of monoclonal antibodies, and particularly discloses a new coronavirus RBD specific monoclonal antibody of which the heavy chain amino acid sequence is shown as SEQ ID NO: 1, the amino acid sequence of the light chain of which is shown as SEQ ID NO: 2. The invention also provides an application of the new coronavirus RBD specific monoclonal antibody. The invention has important scientific significance and application prospect in prevention of diseases caused by the novel coronavirus SARS-CoV-2, clinical treatment and research and development of diagnostic reagents.

Description

technical field [0001] The invention belongs to the technical field of monoclonal antibodies, in particular to novel coronavirus RBD-specific monoclonal antibodies and applications. Background technique [0002] Antibodies are immunoglobulin molecules composed of four polypeptide chains, including two heavy chains (H chains) and two light chains (L chains). The H chain consists of a heavy chain variable region (VH) and a heavy chain constant region consisting of three regions CH1, CH2 and CH3. The L chain consists of an L chain variable region (VL) and a light chain constant region consisting of a CL region. VH and VL can be further divided into hypervariable regions called complementarity determining regions (CDRs) and alternately conserved regions called framework regions (FR). [0003] Current research has found that the new coronavirus (SARS-CoV-2) has four main structural proteins, namely spike protein (S protein), nucleocapsid protein (N protein), membrane protein (M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C12N15/13G01N33/577G01N33/569A61K39/42A61P31/14
CPCC07K16/10G01N33/577G01N33/56983A61P31/14G01N2333/165A61K2039/505C07K2317/51C07K2317/515G01N2469/10Y02A50/30
Inventor 金艾顺韩晓建李婷婷胡超黄晶晶申美莹
Owner CHONGQING MEDICAL UNIVERSITY
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