A method for quantitative analysis of the relationship between plant stomatal closure and root genotype using reciprocal grafts

Abstract

The invention provides a method for quantitatively analyzing the relationship between plant stomatal closure and root genotype by using mutual grafts, using two plants and their mutual grafts as analysis materials, and measuring soil moisture at preset time intervals under drought conditions Content and lysimeter unit weight, calculate the instantaneous transpiration rate, take the daily maximum transpiration rate as the ordinate, and take the corresponding soil water content as the abscissa, respectively draw the scatter diagram of the daily maximum transpiration rate of the four materials with the change of soil water content , using nonlinear curve fitting method to fit the transpiration rate change curve segmentedly, the soil water content corresponding to the inflection point is the soil moisture critical value corresponding to the plant stomata closing, comparing the soil moisture critical value of the four materials, to To determine the relationship between plant stomatal closure and root genotype. The invention can quantitatively analyze the relationship between plant stomatal closure and root genotype, and is beneficial to carry out targeted precise regulation and improvement of drought resistance according to the characteristics of different plants.

Description

technical field [0001] The invention relates to the technical field of plant biotechnology, in particular to the technical field of the relationship between plant stomatal closure and root genotype, and specifically refers to a method for quantitatively analyzing the relationship between plant stomatal closure and root genotype by using mutual grafts. Background technique [0002] The increasing soil drought around the world has posed a huge threat to agricultural production. Roots and leaves are the main organs of the underground and aboveground parts of the plant respectively. Plants absorb water and mineral elements through the roots, and transpiration through the stomata of the leaves creates a water potential difference between the aboveground and underground parts, maintaining the power of the root system to absorb water; stomata are also CO 2 access to the plant body. Roots and shoots interact to regulate the physiological functions of plants under different soil moi...

AI Technical Summary

Problems solved by technology

However, the current studies on the relationship between soil drought-root signal-stomatal closure are all qualitative or semi-quantitative methods, and it is still unclear whether the closure of stomata in the process of soil dehydration depends only on the signal transmitted by the root. distinguish

However, this question is of great value in production, because if it can be determined that the sensitivity of stomatal responses to drought is determined solely by root genotype, it will be possible to selectively apply regulators to plant roots to regulate stomata in agricultural production, In order to balance plant growth and drought resistance; on the contrary, it is necessary to consider the interaction between roots and shoots, and adopt more complex regulatory strategies

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