A method for efficiently inducing polyploidy in ginseng

A native ginseng and polyploid technology, applied in the field of efficient polyploid induction of native ginseng, can solve problems such as genetic stenosis

Active Publication Date: 2021-08-20
CHINESE ACAD OF TROPICAL AGRI SCI +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, study the polyploid induction technology of soil ginseng, and propose a method for efficiently inducing polyploidy of soil ginseng. Obtaining polyploid soil ginseng is of great significance for its use in vegetable production, medical application, and soil stabilization, which will change the existing Soil ginseng has a single variety and relatively narrow genetics

Method used

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  • A method for efficiently inducing polyploidy in ginseng
  • A method for efficiently inducing polyploidy in ginseng
  • A method for efficiently inducing polyploidy in ginseng

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1 - a kind of method for efficiently inducing polyploidy of soil ginseng, comprising the following steps:

[0030] (1) Sterilization treatment: take soil and cut the stem section of ginseng tissue cultured seedling with bud point into 0.8cm long, and the number of bud points of the stem section with bud point is 31; 2 Sterilize for 10 minutes, rinse with sterile water 5 times;

[0031] (2) Polyploid induction: Soak the sterilized stem section with buds in a colchicine culture solution with a mass concentration of 1 mg / ml, and after induction treatment for 24 hours, use 60 Coγ-ray irradiation, the irradiation dose is 10Gy, and the radiation dose rate is 2Gy / min;

[0032] (3) Tissue culture seedlings:

[0033] The first stage: quickly transfer the irradiated stems with buds to the 1 / 2MS medium with a mass concentration of 5 mg / L colchicine culture solution for 3 days in dark co-cultivation, pH 5.6-6.0;

[0034] The second stage: Transfer to the 1 / 2MS medium ...

Embodiment 2- 1

[0037] Embodiment 2-a method for efficiently inducing polyploidy of ginseng, comprising the steps of:

[0038] (1) Sterilization treatment: get soil ginseng tissue cultured seedlings with bud stems and cut them into 1.2cm long, the number of buds in the stems with buds is 33; 2 Sterilize for 12 minutes, wash with sterile water 6 times;

[0039] (2) Polyploid induction: Soak the sterilized stem section with buds in a colchicine culture solution with a mass concentration of 3 mg / ml, and after induction treatment for 48 hours, use 60 Coγ-ray irradiation, the irradiation dose is 15Gy, and the radiation dose rate is 3Gy / min;

[0040] (3) Tissue culture seedlings:

[0041] The first stage: quickly transfer the irradiated stems with buds to the 1 / 2MS medium with a mass concentration of 10 mg / L colchicine culture solution for co-cultivation in the dark for 5 days, pH 5.6-6.0;

[0042]The second stage: Transfer to the 1 / 2MS medium with a mass concentration of 1.5mg / L colchicine, 0.5...

Embodiment 3- 1

[0045] Embodiment 3 - a method for efficiently inducing polyploidy of ginseng, comprising the following steps:

[0046] (1) Sterilization treatment: get soil ginseng tissue cultured seedlings with bud stems and cut them into 1.0cm long, the number of buds in the stems with buds is 32, after 0.2% HgCl 2 Sterilize for 11 minutes, wash with sterile water 6 times;

[0047] (2) Polyploid induction: Soak the sterilized stem section with buds in a colchicine culture solution with a mass concentration of 1 mg / ml, and after induction treatment for 72 hours, use 60 Coγ-ray irradiation, the irradiation dose is 12Gy, and the radiation dose rate is 3Gy / min;

[0048] (3) Tissue culture seedlings:

[0049] The first stage: quickly transfer the irradiated stems with buds to the 1 / 2MS medium with a mass concentration of 7.5 mg / L colchicine culture solution for co-cultivation in the dark for 4 days, pH 5.6-6.0;

[0050] The second stage: transfer to the 1 / 2MS medium with a mass concentration...

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Abstract

The invention provides a method for efficiently inducing polyploidy of native ginseng, comprising the following steps: (1) cutting the stem segments with bud points of soil ginseng tissue culture seedlings, and sterilizing them; (2) using 1-3 mg / ml of autumn Soak in narcissin medium, induce treatment for 24-72 hours, and irradiate with 60Co γ-ray; (3) quickly transfer to 1 / 2MS medium containing 5-10 mg / L colchicine medium for co-cultivation in the dark; 1 / 2 MS medium containing NAA, 6-BA and 6-BA for light culture; transfer to the MS medium containing NAA, 6-BA and tryptophan for light culture to obtain native ginseng tissue culture seedlings, and ploidy identification; the present invention The high mutation rate and high seedling emergence rate induced by polyploidy of native ginseng were effectively realized, a stable and efficient polyploidy induction technology of native ginseng was formed, the mortality of polyploid induced by native ginseng was reduced and the quality of seedling emergence was improved.

Description

technical field [0001] The invention relates to the technical field of native ginseng polyploid breeding, in particular to a method for efficiently inducing polyploidy of native ginseng. Background technique [0002] Soil ginseng, also known as jade ginseng, flying ginseng, green orchid, etc., is an annual or perennial herb of the family Portulaca oleracea. It is native to tropical America, distributed in West Africa, tropical South America and Southeast Asia, and cultivated in central and southern China to Taiwan. The utilization value of soil ginseng is very extensive, mainly in the following aspects: (1) soil ginseng is a delicacy on the table, it is not only rich in nutrition, unique in flavor, but also rich in amino acids, minerals and vitamins; (2) soil ginseng is a delicacy on the table. Ginseng is also a kind of Chinese herbal medicine with medical value. Both its roots and leaves can be used as a medicine, with a flat taste and sweet taste. Soil ginseng has strong...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08A01H4/00
CPCA01H1/08A01H4/001A01H4/008
Inventor 刘莹莹张源源高晰宇叶剑秋温春生符家杰
Owner CHINESE ACAD OF TROPICAL AGRI SCI
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