A method for efficiently inducing polyploidy in ginseng
A native ginseng and polyploid technology, applied in the field of efficient polyploid induction of native ginseng, can solve problems such as genetic stenosis
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] Embodiment 1 - a kind of method for efficiently inducing polyploidy of soil ginseng, comprising the following steps:
[0030] (1) Sterilization treatment: take soil and cut the stem section of ginseng tissue cultured seedling with bud point into 0.8cm long, and the number of bud points of the stem section with bud point is 31; 2 Sterilize for 10 minutes, rinse with sterile water 5 times;
[0031] (2) Polyploid induction: Soak the sterilized stem section with buds in a colchicine culture solution with a mass concentration of 1 mg / ml, and after induction treatment for 24 hours, use 60 Coγ-ray irradiation, the irradiation dose is 10Gy, and the radiation dose rate is 2Gy / min;
[0032] (3) Tissue culture seedlings:
[0033] The first stage: quickly transfer the irradiated stems with buds to the 1 / 2MS medium with a mass concentration of 5 mg / L colchicine culture solution for 3 days in dark co-cultivation, pH 5.6-6.0;
[0034] The second stage: Transfer to the 1 / 2MS medium ...
Embodiment 2- 1
[0037] Embodiment 2-a method for efficiently inducing polyploidy of ginseng, comprising the steps of:
[0038] (1) Sterilization treatment: get soil ginseng tissue cultured seedlings with bud stems and cut them into 1.2cm long, the number of buds in the stems with buds is 33; 2 Sterilize for 12 minutes, wash with sterile water 6 times;
[0039] (2) Polyploid induction: Soak the sterilized stem section with buds in a colchicine culture solution with a mass concentration of 3 mg / ml, and after induction treatment for 48 hours, use 60 Coγ-ray irradiation, the irradiation dose is 15Gy, and the radiation dose rate is 3Gy / min;
[0040] (3) Tissue culture seedlings:
[0041] The first stage: quickly transfer the irradiated stems with buds to the 1 / 2MS medium with a mass concentration of 10 mg / L colchicine culture solution for co-cultivation in the dark for 5 days, pH 5.6-6.0;
[0042]The second stage: Transfer to the 1 / 2MS medium with a mass concentration of 1.5mg / L colchicine, 0.5...
Embodiment 3- 1
[0045] Embodiment 3 - a method for efficiently inducing polyploidy of ginseng, comprising the following steps:
[0046] (1) Sterilization treatment: get soil ginseng tissue cultured seedlings with bud stems and cut them into 1.0cm long, the number of buds in the stems with buds is 32, after 0.2% HgCl 2 Sterilize for 11 minutes, wash with sterile water 6 times;
[0047] (2) Polyploid induction: Soak the sterilized stem section with buds in a colchicine culture solution with a mass concentration of 1 mg / ml, and after induction treatment for 72 hours, use 60 Coγ-ray irradiation, the irradiation dose is 12Gy, and the radiation dose rate is 3Gy / min;
[0048] (3) Tissue culture seedlings:
[0049] The first stage: quickly transfer the irradiated stems with buds to the 1 / 2MS medium with a mass concentration of 7.5 mg / L colchicine culture solution for co-cultivation in the dark for 4 days, pH 5.6-6.0;
[0050] The second stage: transfer to the 1 / 2MS medium with a mass concentration...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com