Preparation method of honeysuckle fermented product and application of honeysuckle fermented product in improving skin appearance and resisting aging

A technology of honeysuckle and fermented products, applied in skin diseases, skin care preparations, medical preparations containing active ingredients, etc., can solve the problems of decreased water retention capacity, sensitivity to allergies, etc., to prolong life, improve reducing activity, and increase waste Degradation effect

Active Publication Date: 2020-11-24
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Based on the above, in order to improve the skin’s fragility and sensitivity caused by damaged keratinocytes, decreased water retention capacity, and skin aging, it is necessary to develop a method that can directly and effectively improve or delay the occurrence of skin aging, while at the same time It is necessary to effectively maintain the normal physiological metabolism of keratinocytes, maintain the integrity of the stratum corneum structure, and increase the active ingredients of anti-glycation activity

Method used

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  • Preparation method of honeysuckle fermented product and application of honeysuckle fermented product in improving skin appearance and resisting aging
  • Preparation method of honeysuckle fermented product and application of honeysuckle fermented product in improving skin appearance and resisting aging
  • Preparation method of honeysuckle fermented product and application of honeysuckle fermented product in improving skin appearance and resisting aging

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Embodiment 1 The preparation method of honeysuckle fermented product of the present invention

[0051] In one embodiment of the present invention, the flower of honeysuckle is uniformly mixed with water, alcohol, or an extraction solvent of a mixture of alcohol and water in a ratio of 1:10-20 (w / w), the extraction solvent is preferably water, and the extraction solvent is extracted at 50 After simultaneous sterilization and extraction at -100°C for 0.5-1.5 hours, add 5-10% (w / w) glucose according to the total weight to obtain a honeysuckle extract, and then cool the honeysuckle extract to room temperature for subsequent three stages The following three types of bacteria are used for fermentation in sequence: yeast, lactic acid bacteria, and acetic acid bacteria. The fermentation order of these three bacteria cannot be reversed, and the fermentation time ratio is 1-2.5:1-3:3-10 . In a preferred embodiment of the present invention, first implant 0.01-0.5% (w / w) yeast (Sa...

Embodiment 2

[0052] Example 2 The three-stage fermentation method of the present invention improves the efficacy of total polyphenols in honeysuckle fermented products

[0053] One embodiment of the present invention is to carry out the test experiment of the three-stage fermentation method of the present invention to improve the effectiveness of total polyphenols in the fermented product of honeysuckle, so the Folin-Ciocalteu colorimetric method is used to measure the total polyphenol content in the proxies; The determination method is carried out by utilizing the antioxidant properties of polyphenols. Phosphotungstic acid and phosphomolybdic acid in the reagent are reduced by polyphenols (by Mo 6+ becomes Mo 5+ ) will generate a blue compound (absorbance value at 750nm), and the depth of the blue compound is directly proportional to the total polyphenol content, so it can be used to quantify the total polyphenol content in the analyte.

[0054] First, gallic acid (Gallic acid, purchased...

Embodiment 3

[0059] Example 3 The effectiveness of the three-stage fermentation method of the present invention in improving the reducing activity of honeysuckle fermented products

[0060] One embodiment of the present invention is to carry out the test experiment of the efficacy of the three-stage fermentation method of the present invention to improve the reducing activity of the honeysuckle fermented product of the present invention, so the colorimetric method of reducing the ability of iron oxidation (Ferric reducing antioxidantpower, FRAP) is used, To determine the antioxidant capacity of the analyte; wherein, the use of the analyte with reducing activity can convert iron ions (Fe 3+ ) reduced to ferrous ion (Fe 2+ ), and the solution color changes from reddish brown to green, and the depth of the green compound is directly proportional to the reducing ability, so it can be used to quantify the reducing activity of the analyte.

[0061] First, a standard curve was prepared using asc...

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Abstract

The invention relates to the field of plant extracts, in particular to a preparation method of a honeysuckle fermented product and application of the honeysuckle fermented product in improving skin appearance and resisting aging. The invention provides a honeysuckle fermented product for delaying aging and a preparation method thereof. The honeysuckle fermented product is obtained by extracting honeysuckle with a solvent to obtain a honeysuckle extract, and then sequentially carrying out three-stage fermentation on the honeysuckle water extract with saccharomycetes, lactic acid bacteria and acetic acid bacteria. The invention also provides an application of the honeysuckle fermented product in preparation of a composition for improving the ageing resistance. The preparation method disclosed by the invention can improve the content of total polyphenol, the reduction activity, the antioxidant activity and the capacity of removing final glycosylation products in the honeysuckle extract, and can remarkably improve the expression quantity of SOD3 genes, Parkin genes, Atg1 genes and Ubl-5 genes in skin cells; meanwhile, the expression quantity of the PARP2 gene is reduced.

Description

technical field [0001] The invention relates to the field of plant extracts, in particular to a preparation method of honeysuckle fermented product and its application for improving skin appearance and anti-aging. Background technique [0002] The epidermis is the outermost layer of the skin. From the outside to the inside, it consists of the stratum corneum, granular layer, spinous layer, and basal layer. The epidermis is mainly formed by continuous upward differentiation of undifferentiated cylindrical keratinocytes in the basal layer. The process is called keratinization. The water content in corneocytes is high. As the cells metabolize and differentiate upward, the shape of corneocytes will gradually become flat, and the nucleus and organelles will begin to degenerate and shrink, and dead cells without nuclei and organelles will form in the stratum corneum. The main function of the epidermis is to keep the skin water and form a skin barrier to resist various external in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/355A61P17/18A61K8/9789A61Q19/08A23L33/105
CPCA61K36/355A61P17/18A61K8/9789A61Q19/08A23L33/105A61K2236/19A61K2800/85A23V2002/00A23V2200/302A23V2250/21
Inventor 林咏翔
Owner TCI CO LTD
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