Method for screening rifamycin SV high-yielding strain through oxygen stress mutagenesis

Abstract

The present invention discloses a method for screening a rifamycin SV high-yielding strain through oxygen stress mutagenesis. The method is an efficient breeding method of the rifamycin SV hypoxia-tolerant high-yielding strain based on combination of an oxygen limitation model and low-temperature plasma mutagenesis. The method comprises the following steps: during a process of breeding the rifamycin SV production strain of nocardia mediterranean, oxygen limitation model plates are used to screen the strain that can grow rapidly and has dark brown moss under hypoxia; 0.8-1.2 g/L of anhydrous sodium sulfite is added in a screening culture medium to prepare screening plates, a bacterial suspension with a low-temperature plasma mutagenesis treatment time of 22-28 seconds is diluted and spreadon an oxygen limitation plate culture medium and cultured at 28 DEG C for 6-7 days, the strain with faster growth and darker color is picked, and positive mutation rate of screening can be significantly increased. The method can screen the high-efficiency rifamycin production strain capable of rapid growth and product synthesis under the hypoxic conditions, and the strain is well adapted to the oxygen-limitation environment of the production process and also exhibits good fermentation production capacity.

Description

technical field [0001] The invention relates to a method for screening high-production strains of rifamycin SV, in particular to a method for screening high-production strains of rifamycin SV by oxygen stress mutagenesis. Background technique [0002] Rifamycin SV antibiotics are a class of antibiotics produced by Streptococcus mediterranei. The role of Staphylococcus aureus is very strong. Also effective against some Gram-negative bacteria. Rifamycin SV drugs include: rifamycin SVB diethylamide, rifampin etc. Rifampicin, rifapentine and rifabutin are currently in clinical use. The structural formula of rifamycin SV is as follows: [0003] [0004] In the current rifamycin SV batch culture fermentation process, most of them only use pH, DO, temperature, etc. as the basis and means of control strategies. In most cases, these parameters can only reflect the performance or operating conditions of the equipment, but cannot reflect the physiological state of microorganism...

AI Technical Summary

Problems solved by technology

[0008] Above these technologies are for how to provide a kind of method of oxygen stress mutagenesis screening rifamycin SV high-yielding bacterial strain, this method can be screened under hypoxic condition and can grow rapidly and carry out the efficient rifamycin producing bacterial strain of product synthesis, can very well Well adapted to the oxygen-limited environment of the production process, and showed good fermentation production capacity, no specific guidance plan was given

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