Sites on cat genes related to equine adaptation to hypoxic environments and their applications

An oxygen environment, adaptive technology, applied in the field of genetic engineering and genetic engineering

Active Publication Date: 2022-07-12
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] So far, there has been no report on the use of the equine CAT gene as a molecular marker for equine tolerance to plateau hypoxic environments

Method used

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  • Sites on cat genes related to equine adaptation to hypoxic environments and their applications
  • Sites on cat genes related to equine adaptation to hypoxic environments and their applications
  • Sites on cat genes related to equine adaptation to hypoxic environments and their applications

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Experimental program
Comparison scheme
Effect test

Embodiment

[0029] 1. Experimental animals

[0030] There are 100 experimental horses used in the present invention, mainly from Qusong (4500m, 20 horses), Diqing (3500m, 10 horses), Zuogong (3500m, 10 horses), Laojun Mountain (2500m, 10 horses), Lijiang (2500m, 10 horses), Yimen (1500m, 10 horses), Zhaotong (1500m, 10 horses), Pingbian (500m, 10 horses), Yingjiang (500m, 10 horses), blood samples were collected, and blood samples were collected. Whole-genome resequencing of genomic DNA.

[0031] Using Diqing Tibetan horses (116 horses) and Yingjiang local horses (40 horses) as materials, blood samples were collected to detect plasma catalase (CAT), hydrogen peroxide (H 2 O 2); extract the whole blood genome, design primers for the 10th exon sequence of the horse CAT gene, and sequence the product after PCR amplification to detect the genotype; finally, use the genotype and CAT enzyme activity and H 2 O 2 level correlation analysis.

[0032] 2. Sample Collection

[0033] (1) Blood s...

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PUM

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Abstract

The present invention relates to equine hypoxic environmental adaptability related to CAT The locus on the gene and its application belong to the field of genetic engineering and genetic engineering, and the present invention provides high CAT enzyme activity and low H 2 O 2 level dependent CAT Amino acids 410 (Agt→Ggt, Ser→Gly, serine→glycine) and 421 amino acids (Cgt→Agt, Arg→Ser, arginine→serine) of the gene were mutated, and the two sites are located at CAT A→G mutation at 34bp and C→A mutation at 67bp on exon 10 of gene. The two sites were significantly correlated with high CAT enzyme activity in horses (P<0.01) and low H 2 O 2 The levels were significantly correlated (P<0.05), and the allele G at the 34 bp was the dominant allele of high CAT enzyme activity in horses, and the allele A at the 67 bp was the low H in horses 2 O 2 Horizontally dominant allele. The two loci can be used as molecular markers for detecting high altitude hypoxia tolerance and high altitude adaptation in horses, breeding horse breeds that are resistant to high altitude hypoxia environment, and preparing kits for detecting horse hypoxia environment adaptability.

Description

technical field [0001] The invention belongs to the field of genetic engineering and genetic engineering, and relates to a site on CAT gene related to the adaptability of horses with low oxygen environment and its application, and in particular, relates to high CAT enzyme activity and low H in horses. 2 O 2 Levels of significantly correlated loci on CAT genes and their applications. Background technique [0002] In ancient times, horses used to be the main labor force for agricultural production, transportation and military warfare. With the development of productivity and the improvement of scientific and technological level, the role of horses has been gradually replaced by machines, but in the high altitude and low oxygen or inaccessible areas of the Qinghai-Tibet Plateau with low productivity, horses are still the main means of transportation and labor. [0003] Areas above 2500 m above sea level are defined as plateaus because this is the altitude at which arterial ox...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/156C12Q2600/124C12Q2600/172
Inventor 孔小艳朱莉董新星杨舒黎苟潇张晓燕滕欢
Owner YUNNAN AGRICULTURAL UNIVERSITY
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