Chestnut somatic embryo development synchronization method and tissue culture seedling rooting method

A somatic embryo, synchronization technology, applied in the field of plant tissue culture, can solve the problems of difficult rooting, low synchronization rate, and inability to achieve large-scale asexual reproduction of fine varieties, and achieve the effect of promoting rooting and improving rooting rate.

Active Publication Date: 2020-12-04
BEIJING UNIV OF AGRI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the rapid propagation of good varieties of chestnut is particularly important, but up to now, the breeding of good varieties such as Yanshan red chestnut has always adopted the method of grafting, which cannot realize large-scale asexual reproduction of good varieties
However, the regeneration of somatic embryos can quickly realize the mass reproduction of fine Chinese chestnut varieties. At present, the regeneration system of chestnut somatic embryos has been preliminarily established, but the existing regeneration system of chestnut somatic embryos has the problems of low synchronization rate and difficulty in rooting.

Method used

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  • Chestnut somatic embryo development synchronization method and tissue culture seedling rooting method
  • Chestnut somatic embryo development synchronization method and tissue culture seedling rooting method
  • Chestnut somatic embryo development synchronization method and tissue culture seedling rooting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0112] (1) Plant material

[0113] Yanshan red chestnut (C. mollissima cv. Yanshanhongli) was collected from Chestnut Experimental Station, Huairou District, Beijing. The sampling period of chestnut is 45-54 days after flowering, and immature young embryos are selected.

[0114] (2) Induction of chestnut embryogenic callus

[0115] Take the immature embryo tips of chestnut, sterilize them with 3% sodium hypochlorite and 75% alcohol, and inoculate them on the IMM solid medium. When the callus grows to 5 mm, put the callus in the liquid IMM medium for 7 days. weeks, and finally transferred to E2 solid medium for culture until embryogenic callus was formed.

[0116] (3) Culture of Chestnut Somatic Embryo Synchronization

[0117] a. Inoculate 0.1 g of embryogenic callus tissue into a 250 mL sterile Erlenmeyer flask, and add 100 mL of E1 liquid medium. After cultivating on a shaker for 7 days, filter the suspension culture with 20-mesh and 40-mesh sieves successively, Retain th...

Embodiment 2

[0137] The cotyledon-shaped embryos in Example 1 are induced to obtain tissue cultured plantlets, and then the Chinese chestnut tissue cultured plantlets with good growth status are inoculated into the rooting medium for cultivation, wherein the components of the rooting medium include: 2.3g / L WPM , 30g / L sucrose, 0.1mg / L IBA and 6.5g / L agar (pH 5.5). Chestnut tissue culture seedlings were cultured at 23-25°C with a photoperiod of 16h / 8h. Repeat three times, count the rooting situation and analyze the data after 25 days of cultivation.

Embodiment 3

[0139] The difference with Example 2 is that the concentration of IBA in the rooting medium is 0.2mg / L.

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Abstract

The invention provides a chestnut somatic embryo development synchronization method and a tissue culture seedling rooting method, and relates to the technical field of plant tissue culture. Accordingto the chestnut somatic embryo development synchronization method, liquid suspension culture is conducted on embryonic calluses, the embryonic calluses with specific volumes and sizes in different development periods are screened, and the embryonic calluses with the consistent growth state are obtained; and spherical embryos, heart-shaped embryos, torpedo-shaped embryos and cotyledon-shaped embryos which are consistent in growth state are obtained by applying the method. The synchronization rate of the spherical embryos is 91.82%, the synchronization rate of the heart-shaped embryos is 83.92%,the synchronization rate of the torpedo-shaped embryos is 76.69%, the synchronization rate of the cotyledon-shaped embryos is 69.52%, and synchronization of the chestnut somatic embryos in all stagesin the development process is basically achieved. The invention also provides the tissue culture seedling rooting method which can promote rooting of tissue culture seedlings and improve the rootingrate.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for synchronizing the development of chestnut somatic embryos and a method for rooting tissue culture seedlings. Background technique [0002] Chestnut (Castanea Mollissima) is a plant of the genus Chestnut in the family Fagaceae, and is an important ecological and economic forest tree species in my country. Chestnut has strong disease resistance, less insect damage, barren tolerance, and drought tolerance, and plays an important role in afforestation and greening and ecosystem services. Chestnut production ranks first in the world's nut production, has important economic value, and has the laudatory title of "king of dried fruits". [0003] Yanshan red chestnut (C. mollissima cv. Yanshanhongli) is one of the fine varieties suitable for planting in the area north of the Qinling Mountains in my country. Yanshan red chestnut has the characteristics of strong...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 曹庆芹秦岭孙芝林李晓伟房克凤邢宇张卿
Owner BEIJING UNIV OF AGRI
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