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Preparation method of COVID-19 vaccine based on exosome platform

A virus vaccine and exosome technology, applied in the fields of genetic engineering and biomedicine, can solve problems limited to small-scale basic research and achieve the effect of realizing therapeutic potential

Inactive Publication Date: 2020-12-22
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Despite the success of many other drugs in preclinical testing, they are still limited to small basic studies

Method used

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  • Preparation method of COVID-19 vaccine based on exosome platform

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Embodiment Construction

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] see figure 1 , the present invention provides a technical solution: a method for preparing a novel coronavirus vaccine based on an exosome platform, comprising the following steps:

[0026] Step 1: Construct the Spike-lamp2b recombinant plasmid;

[0027] Step 2: Extracting secreted exosomes in the cell supernatant;

[0028] Preferably, said step one specifically includes the following operations:

[0029] S1: Design primers for lamp2b, use mouse cDNA...

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Abstract

The invention relates to the field of gene engineering and biomedicine, in particular to a preparation method of a COVID-19 vaccine based on an exosome platform. The preparation method comprises the following steps: step I, constructing Spike-lamp2b recombinant plasmids; and step II, extracting exosomes secreted in cell supernatant. Compared with the prior art, the preparation method has the beneficial effects that the therapeutic potential of a vaccine medicine of the exosome platform is realized, and the vaccine medicine is efficiently delivered in a tissue-specific manner to be used for preventing the COVID-19.

Description

technical field [0001] The invention relates to the fields of genetic engineering and biomedicine, in particular to a method for preparing a novel coronavirus vaccine based on an exosome platform. Background technique [0002] The novel coronavirus (COVID-19) is spreading globally. At the moment of the outbreak, the most urgent thing is to find testing reagents and effective treatment drugs, but in the long run, vaccines are the ultimate weapon to defeat emerging infectious diseases. In particular, the novel coronavirus pneumonia (COVID-19) may exist for a long time as influenza, and vaccines will become more and more important in future epidemic prevention work. More than 140 SARS-CoV-2 vaccines are currently under accelerated development, and an effective and safe vaccine will be a major breakthrough in the fight against COVID-19. [0003] The 2019 novel coronavirus (SARS-CoV-2) belongs to the family Coronaviridae of the order Nidoviridae. It is a type of virus that is sp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N5/10A61K39/215A61P31/14
CPCA61K39/12A61K2039/5154A61P31/14C07K14/005C07K14/47C07K2319/00C12N5/0639C12N15/85C12N2510/00C12N2770/20022C12N2770/20034C12N2800/107
Inventor 段莉梁宇杰徐晓夏江王大平
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN