Unlock instant, AI-driven research and patent intelligence for your innovation.

A functional gene chip for identifying medicinal plant species and/or screening potential medicinal ingredients and its preparation and use method

A technology for functional genes and medicinal plants, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., which can solve the problems of difficult classification and identification, low efficiency, etc.

Active Publication Date: 2022-05-10
CENT SOUTH UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to construct a gene chip containing a large amount of medicinal ingredient information by designing 144 probes of multiple functional genes, so as to hybridize the chip with the genomic DNA of the plant sample to be tested, and realize the rapid identification of the species of medicinal plants, and Screen potential medicinal ingredients to solve the shortcomings of traditional methods, such as low efficiency and difficult classification and identification

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A functional gene chip for identifying medicinal plant species and/or screening potential medicinal ingredients and its preparation and use method
  • A functional gene chip for identifying medicinal plant species and/or screening potential medicinal ingredients and its preparation and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Preparation of functional gene probes

[0025] Find the metabolic pathways of alkaloids, flavonoids, phenylpropanoids, quinones, steroids, terpenes, etc. in the known KEGG (www.kegg.jp) database, and find the key genes corresponding to typical Chinese medicine ingredients, Combining with the Genbank database, the screening obtained distribution in 17 genera (Vinca, Ginkgo, Astragalus, Eucommia, Artemisia, Coptidis, Heliconia, Mentha, Taxus, Rauwolfia, Stevia , Glycyrrhizae, Crocus, Foxglove, Saussurea, Scutellaria, Panax) 311 sequences of 160 functional genes in 32 species, and their numbers in Genbank are as follows:

[0026] Alkaloids

[0027] AAR02419.1, AAD42938.1|AF084972_1, Q39522, Q8W013, AAK20170.1, 2FP9_A, 2FPC_A, 2FPB_A, 2FP8_A, 2FP9_B, 2FPB_B, 2FP0_B, 2FPC_B, BAB71802.1, BAC22084.1, CAB565 1, AAC99311.1, O04847, AAC49827.1, AAC49826.1, AAB97311.1, AAR09601.1, AAR02420.1, AAR09602.1, AAR09599.1, AAR09598.1, AAR09600.1, AAM97497.1, AAK1479 1, AAD42937.1|AF08...

Embodiment 2

[0052] Fluorescent labeling of periwinkle DNA samples

[0053] As described in Example 1, mix 1.500 μg of periwinkle (Catharanthus roseus) sample DNA with 1.5 μg of hexamer base random primers to form a 25 μL system, denature at 95°C for 2 minutes, and quickly cool on ice to obtain the denatured DNA solution Mix with 15 μL of labeling reaction solution. The components of the labeling reaction solution are as follows: 5mmol / L dATP, dTTP, dGTP and 2.5mmol / LdCTP; 1mmol / L Cy5-dCTP (fluorescence red, excitation wavelength 647nm; emission wavelength 660-705nm); 2.5mmol / L dithiothreose Alcohol (DTT); 10 U Klenow Large Fragment DNA Polymerase I (Invitrogen, Calsbad, CA). The reaction mixture (40 μL) was incubated at 37°C for 2h, denatured on a heating plate at 100°C for 3min, and cooled rapidly on ice. The labeled DNA was then purified with a QIAquick PCR purification column, the purified solution was concentrated at 40°C for 1 h, and the concentrated labeled DNA was redissolved wit...

Embodiment 3

[0058]According to the conditions described in Example 2, Eucommia ulmoides (Eucommiaulmoides) was subjected to the same treatment, hybridization and detection, and obtained 6 hybridization points with an SNR value greater than 2, and the fingerprints of 4 repetitions were as follows: figure 2 shown.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a functional gene chip for identifying medicinal plant species and / or screening potential medicinal ingredients and its preparation and application method. The method constructs a functional gene chip composed of 144 probes, and through hybridization of the chip with the genomic DNA of the plant sample to be tested, the species of medicinal plants can be quickly identified, and the purpose of screening potential medicinal ingredients can be achieved. Compared with traditional medicinal plant classification and medicinal component identification methods, the present invention realizes rapid identification of medicinal plant specimens under natural wild conditions and simultaneous analysis of medicinal component analysis, as well as medicinal plant resource information-specimen-object - An integrated system of fingerprints.

Description

technical field [0001] The invention belongs to the technical field of identification of medicinal plants, and in particular relates to a gene chip for identification of medicinal plant species and for screening potential medicinal components and its preparation and use methods. Background technique [0002] Traditional Chinese medicine has been used for thousands of years. It has a wide variety of plant bodies and complex medicinal ingredients. It exerts its efficacy through multiple components, multiple targets, and multiple channels. Therefore, the mechanism is complicated and it is difficult to study thoroughly. Research poses great difficulties. [0003] The traditional classification and identification of medicinal plants is based on the phenotypic characteristics of the plants, including growth habits, branch and leaf characteristics, etc., which cannot be classified and identified very well. However, gene chips design probes based on a large amount of genetic informa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6837C12N15/11
CPCC12Q1/6895C12Q1/6837
Inventor 刘学端梁伊丽邹凯杨琴扶绍东廖立琴
Owner CENT SOUTH UNIV