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Method, primer and kit for auxiliary detection of pig backfat thickness character by using pig chromosome 2 gene polymorphic site and application

A polymorphic site, auxiliary detection technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., to achieve the effect of reducing breeding costs, improving breeding progress, and low cost

Pending Publication Date: 2020-12-25
SHANGHAI XINNONG FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the measurement period for backfat thickness up to 100kg body weight is in the range of 85-110kg, which can only be measured after the pig grows up.

Method used

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  • Method, primer and kit for auxiliary detection of pig backfat thickness character by using pig chromosome 2 gene polymorphic site and application
  • Method, primer and kit for auxiliary detection of pig backfat thickness character by using pig chromosome 2 gene polymorphic site and application
  • Method, primer and kit for auxiliary detection of pig backfat thickness character by using pig chromosome 2 gene polymorphic site and application

Examples

Experimental program
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Embodiment 1

[0038]Example 1. Determination of polymorphic sites, and correlation analysis between polymorphic sites and pig backfat thickness traits 1. Identification of polymorphic sites on pig chromosome 2 (v11.1) g.71588075G>A Sure

[0039] 1. PCR amplification

[0040] According to the upstream and downstream sequence information of porcine chromosome 2 (v11.1) g.71588075G>A in the International Porcine Genome Version 11.1 Reference Sequence, a pair of primers were designed and synthesized as follows:

[0041] U (upstream primer): 5'-GAGATGGCTTCACTGAGG-3' (sequence 1 in the sequence listing);

[0042] D (downstream primer): 5'-ATCAACAAGGCTGAGGAC-3' (SEQ ID NO: 2 in the Sequence Listing).

[0043] Large white pigs (2 heads) were selected as experimental materials. Using the genomic DNA of Large White pig as a template, the upstream primer U and downstream primer D were used for PCR amplification. (Choose the cells of the ear tissue of Large White pigs as a biological sample, take t...

Embodiment 2

[0066] Embodiment 2, establishment of detection method (provide primer pair, kit)

[0067] (1) Establish a detection method using the polymorphic site of the pig chromosome 2 gene to assist in the detection of pig backfat thickness traits, that is, a detection method using the pig chromosome 2 g.71588075G>A in the International Porcine Genome Version 11.1 Reference Sequence A method for detecting backfat thickness traits of pigs with a body weight of 100 kg assisted by polymorphic sites. The detection method is: detecting the deoxyribonucleoside at position 71588075 from the 5′ end of pig chromosome 2 (v11.1) of the pig to be tested Whether the acid is G or A, that is, to detect whether the 71588075th deoxyribonucleotide of the pig chromosome 2 in the reference sequence of the international pig genome version 11.1 of the pig to be tested is G or A from the 5′ end, and determine the gene of the pig to be tested Whether the genotype is GG or AA, the backfat thickness of pigs wit...

Embodiment 3

[0080] Embodiment 3, establishment of breeding method (application of detection method, primer pair, test kit)

[0081] The detection method, the primer pair, and the kit described in the above-mentioned Example 2 are applied to pig breeding to breed pigs with thinner backfat thickness traits.

[0082]The method for cultivating pigs with thinner backfat and thick traits: detect whether the 71588075th deoxyribonucleotide of the pig chromosome 2 (v11.1) of the pig to be tested is G or A from the 5' end, and determine whether the pig to be tested is G or A. Whether the genotype is GG or AA; pigs with AA genotype are selected for breeding, and pigs with thinner backfat and thicker traits can be obtained.

[0083] The method for detecting whether the 71588075th deoxyribonucleotide of the pig chromosome 2 (v11.1) of the pig to be tested is G or A from the 5' end is specifically sequencing analysis; the sequencing analysis includes PCR amplification and Steps for sequencing the PCR ...

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Abstract

The invention discloses a method, a primer and a kit for auxiliary detection of pig backfat thickness character by using pig chromosome 2 gene polymorphic site, and an application. The method comprises the following steps of detecting whether deoxyribonucleotide at the 71588075th site of chromosome 2 of a pig to be detected is G or A from the 5' tail end, determining whether the genotype of the pig to be detected is GG or AA, and determining that the backfat thickness of the pig with the GG genotype is higher than that of the pig with the AA genotype; adopting the primer pair for detecting whether the 71588075th deoxyribonucleotide of the pig chromosome 2 of the pig to be detected is G or A from the 5' tail end according to the method, and determining whether the genotype of the pig to bedetected is GG or AA; and selecting pigs with AA genotypes for breeding, and obtaining the pigs with thinner backfat thickness characters. The characters of the pigs are selected by the method, so that the backfat thickness of the pigs can be reduced by about 0.83 cm, and the pigs with higher production performance can be obtained. When the method is used for breeding pigs, the time for selectingexcellent breeding pigs can be shortened, and the breeding cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a method for assisting detection of backfat thickness traits of pigs weighing up to 100 kg by using the 71588075th G>A polymorphic site from the 5' end of pig No. 2 chromosome (v11.1) , primers, special kit and application, that is, a method, primers, kit and application for assisting detection of pig backfat thickness traits by utilizing the polymorphic site of pig chromosome 2 gene. Background technique [0002] The backfat thickness of pigs up to 100kg body weight is not only related to lean meat percentage, but also has a strong correlation with growth rate. Therefore, its value in breeding is very high, and it has always been an important part of pig breeding goals. However, the measurement period for backfat thickness up to 100kg body weight is in the range of 85-110kg for pigs, which can only be measured after the pigs grow up. On the one hand, the determinatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124Y02P60/87
Inventor 宋欣黄黎威马微微方艳红顿涛李会智
Owner SHANGHAI XINNONG FEED