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Antibodies of coronavirus or antigen binding fragments of antibodies

A technology that combines fragments and coronaviruses, applied in the direction of viruses/phages, antibodies, viruses, etc., can solve problems such as mutation of virus genome sequence, loss of protective effect of mutant virus, etc.

Active Publication Date: 2021-01-01
超非凡(上海)医疗科技有限公司
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AI Technical Summary

Problems solved by technology

[0007] Although the antibodies currently reported by other teams have good neutralizing ability to the tested SARS-CoV-2 virus strains, due to the SARS-CoV-2 virus It is an RNA virus, and the genome sequence of the virus is prone to mutations during the spread of the epidemic
When the non-conserved region recognized by these antibodies is mutated to produce a new circulating strain, the antibody will lose its protective effect against the mutant virus

Method used

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  • Antibodies of coronavirus or antigen binding fragments of antibodies
  • Antibodies of coronavirus or antigen binding fragments of antibodies

Examples

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Embodiment 1

[0098] Example 1: Screening and detection of antibodies to coronavirus

[0099] The inventor conducted a pseudovirus assay on the plasma of patients with novel coronavirus pneumonia (followed up two weeks after recovery and discharge) admitted to the inventor's unit (Shanghai Public Health Clinical Center) from January 20, 2020 to February 26, 2020. And experimental screening, it was found that the serum of three mild patients had strong neutralizing activity against the pseudovirus of SARS-CoV-2. With the written consent of the ethics committee of the inventor's unit and the patient himself, the peripheral blood was drawn for research.

[0100] 1. Sorting of peripheral blood memory B cells

[0101] 1) Separation of peripheral blood lymphocytes: extract the convalescent peripheral blood of the above-mentioned patients and mix it with an equal amount of normal saline, then use Lymphoprep (Stemcell Technologies, Cat. No. 07851) to separate peripheral blood lymphocytes. For the o...

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Abstract

The invention relates to antibodies of a coronavirus or antigen binding fragments of the antibodies, a nucleic acid molecule for encoding the antibodies or the antigen binding fragments thereof, a vector including the nucleic acid molecule, a host cell including the vector, and application of the antibodies or the antigen binding fragments thereof to the aspect of preparation of drugs for treatingor preventing diseases caused by the coronavirus, and the aspect of detection products. The inventors utilize a B cell in-vitro monoclonal culture and high-throughput antibody screening technology toobtain a series of antibodies of the coronavirus and the antigen binding fragments of the antibodies, and the antibodies and the antigen binding fragments thereof have high binding capacity and neutralizing capacity for the SARS-CoV-2 virus, can recognize and bind S1 protein of the SARS-CoV-2 virus and a receptor binding domain (RBD) of the S1 protein, and have very strong affinity, and thus, itcan be speculated that the antibodies and the antigen binding fragments possibly have the binding capacity and neutralizing capacity for other coronaviruses, and coronaviruses possibly appearing in the future, and the antibodies and the antigen binding fragments have good clinical application prospects in the future.

Description

technical field [0001] The present invention relates to a coronavirus antibody or an antigen-binding fragment thereof, a nucleic acid molecule encoding the antibody or an antigen-binding fragment thereof, a vector comprising the nucleic acid molecule, a host cell comprising the vector, and preparation of the antibody or an antigen-binding fragment thereof The application of drugs for the treatment or prevention of diseases caused by coronaviruses, and the application of detection products belong to the field of biomedicine. Background technique [0002] Novel coronavirus pneumonia (2019-nCOV) is an acute respiratory infectious disease caused by a new type of coronavirus, SARS-COV-2. The virus is highly transmissible and can be transmitted through the respiratory tract, contact and other channels. Since the outbreak in December 2019, it has spread to all parts of the world, forming a worldwide pandemic. As of July 1, 2020, the SARS-CoV-2 coronavirus has caused more than 10 m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C12N15/13C12N15/867G01N33/569G01N33/577A61K39/42A61P31/14
CPCC07K16/10G01N33/56983G01N33/577A61P31/14C12N2800/107G01N2333/165G01N2469/10A61K2039/505C12N15/85C12N5/0686C07K2317/56C07K2317/565C07K2317/76C12N2510/00
Inventor 黄竞荷吴凡刘梅
Owner 超非凡(上海)医疗科技有限公司
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