Cleaning solution for magnetic particle chemiluminescence immunoassay and preparation method thereof

A technology of chemiluminescent immunity and cleaning solution, which is applied in the field of biotechnology analysis, can solve the problems of poor cleaning effect, affecting sensitivity, and high background value, and achieve the effects of low manufacturing cost, improved sensitivity, and reduced background value

Inactive Publication Date: 2021-01-01
芯朗道(天津)医疗科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inadequate washing can lead to variance and high background, affecting assay sensitivity
[0005] The cleaning solution of the existing magnetic particle chemiluminescence immunoassay has poor cleaning effect and high background value, which affects the sensitivity of detection and even causes false positives

Method used

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  • Cleaning solution for magnetic particle chemiluminescence immunoassay and preparation method thereof
  • Cleaning solution for magnetic particle chemiluminescence immunoassay and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0025] The preparation method of magnetic particle chemiluminescence immunoassay cleaning solution provided by the invention comprises the following steps:

[0026] Step 1: Preparation of 37% HCl: Take 37ml of concentrated hydrochloric acid, add 50ml of ultrapure water, mix well, and then dilute to 100ml;

[0027] Step 2: Weigh 56.2g of Tween 20 and fully dissolve it with 500ml of ultrapure water; then add 154.4g of Tris and 149g of NaCl to fully dissolve; then add 94.4ml of 37% HCl to fully dissolve; finally set the volume to 1L, by adjusting the proportion of the compound in the formula, the best cleaning effect is obtained experimentally.

[0028] Among them, the main components are as follows:

[0029] NaCl, Tris, HCl, Tween 20, and ultrapure water.

[0030] The role of NaCl is to provide a suitable ion concentration to keep the immune complex stable;

[0031] Tris and HCl: Form a Tris-HCl buffer system to keep the solution at a suitable pH value and keep the immune com...

Embodiment 1

[0034] Make other components in the cleaning solution constant, NaCl content is different, to the detection of procalcitonin (PCT), observe the influence of different cleaning solutions on background value and linear width, as shown in Table 1;

[0035] The composition of the cleaning solution for magnetic particle chemiluminescence immunoassay:

[0036] The formula of 1L cleaning solution concentrate (13×) is:

[0037] Tris: 154.4g;

[0038] Nacl: 149g;

[0039] Tween 20: 56.2g;

[0040] 37% HCl: 94.4ml;

[0041] Make up to 1L with ultrapure water.

[0042] The formula of 1L cleaning liquid working liquid is:

[0043] Cleaning solution concentrate (13×): 76.923ml;

[0044] Make up to 1L with ultrapure water.

[0045] The detection of procalcitonin (PCT) in table 1, the influence (n=10, )

[0046]

[0047] Remarks: The concentration of PCT in sample A is 0ng / ml, the concentration of sample B is 0.25ng / ml, and the concentration of sample C is 100ng / ml.

Embodiment 2

[0049] Make other components in the cleaning solution constant, Tween content is different, to the detection of procalcitonin (PCT), observe the influence of different cleaning solutions on background value and linear width, as shown in Table 2;

[0050] The composition of the cleaning solution for magnetic particle chemiluminescence immunoassay:

[0051] The formula of 1L cleaning solution concentrate (13×) is:

[0052] Tris: 154.4g;

[0053] Nacl: 149g;

[0054] Tween 20: 56.2g;

[0055] 37% HCl: 94.4ml;

[0056] Make up to 1L with ultrapure water.

[0057] The formula of 1L cleaning liquid working liquid is:

[0058] Cleaning solution concentrate (13×): 76.923ml;

[0059] Make up to 1L with ultrapure water.

[0060] The detection of procalcitonin (PCT) in table 2, the influence (n=10, )

[0061]

[0062] Remarks: The concentration of PCT in sample A is 0ng / ml, the concentration of sample B is 0.25ng / ml, and the concentration of sample C is 100ng / ml.

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Abstract

The invention relates to the field of biotechnology analysis, and discloses a cleaning solution for magnetic particle chemiluminescence immunoassay, which comprises an immune complex retention stabilizer, a nonionic surfactant, a buffer agent and ultrapure water, wherein the immune complex retention stabilizer is a sodium chloride solvent, the nonionic surfactant is Tween 20, and the buffer agentis a Tris-HCl buffer agent, so that the background value of a detection result can be remarkably reduced, and the detection sensitivity is improved; in addition, the invention provides a preparation method of the cleaning solution for magnetic particle chemiluminescence immunoassay, wherein the formula is simple, the raw materials are easy to obtain, and the shelf life of the prepared cleaning solution is long.

Description

technical field [0001] The invention relates to the field of biotechnology analysis, in particular to a cleaning solution for magnetic particle chemiluminescence immunoassay. Background technique [0002] Magnetic particle chemiluminescence immunoassay technology combines magnetic particle carrier technology and chemiluminescence immunoassay technology. It has the advantages of high sensitivity, wide detection range and short detection time. It is a new in vitro detection technology. [0003] The detection steps of the magnetic particle chemiluminescent immunoassay technique are: the antigen (antibody) to be detected is combined with the antibody (antigen) to form a magnetic particle-enzyme-linked immune complex, and then the complex is washed multiple times with a cleaning solution to remove For the unbound enzyme-linked antibody (antigen), the complex is finally added to the luminescent substrate, and the enzyme-catalyzed substrate emits fluorescence, and the luminescence ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11D1/74C11D3/04C11D3/30G01N21/76G01N33/53
CPCC11D1/74C11D3/046C11D3/30G01N21/76G01N33/53
Inventor 刘鹤冯君君
Owner 芯朗道(天津)医疗科技有限责任公司
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