Anti-interference electrochemical uric acid test paper and preparation method thereof

An electrochemical and test paper technology, which is applied in the field of anti-interference electrochemical uric acid test paper and its preparation, can solve the problems of not being able to significantly improve the anti-interference ability of the test strip, poor anti-interference effect, and poor reaction specificity, so as to speed up the transfer of electrons. The effect of conduction rate, strong conductivity, and high sensitivity

Pending Publication Date: 2022-03-25
NANJING EAGLENOS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can avoid superimposing two kinds of water-soluble and non-water-soluble interfaces in the above method, it can avoid the problems of slow reaction speed, long additional measurement time and poor reaction specificity.
However, the anti-interference effect of this method is very poor, and the interfering substance and the substance to be tested react with the electron mediator at the same time, which cannot significantly improve the anti-interference ability of the test strip ascorbic acid

Method used

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  • Anti-interference electrochemical uric acid test paper and preparation method thereof
  • Anti-interference electrochemical uric acid test paper and preparation method thereof
  • Anti-interference electrochemical uric acid test paper and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 Structure, composition, preparation and performance test of electrochemical uric acid test paper.

[0043] Schematic diagram of the structural decomposition of the electrochemical uric acid test paper of the present embodiment figure 1 shown.

[0044] The preparation method step of electrochemical uric acid test paper strip of the present invention is as follows:

[0045] Preparation of the metal electrode: take the polymer insulating substrate (1), and vacuum-sputter 20nm pure gold on the insulating substrate, and engrave the working electrode (3), reference electrode (4), The electrode system such as the detection electrode (5) and the connecting electrode (6) of sample injection.

[0046] Preparation of basic buffer: prepare ACES buffer solution with a concentration of 0.2M and a pH value of 7.0, and weigh 1% of trehalose, 1% of triton, 2% of bovine serum albumin, and 4% of hydroxyethyl cellulose , mix until completely dissolved.

[0047] Preparatio...

Embodiment 2

[0060] Example 2: Comparison experiment of anti-interference ability of electrochemical uric acid test strips

[0061] In this example, in order to compare the existence of the anti-interference area, the anti-interference ability against different concentrations of ascorbic acid in the blood sample. The preparation method of the anti-interference uric acid test paper of this example and the adoption of no anti-interference uric acid test paper is as follows:

[0062] Preparation of the metal electrode: take the polymer insulating substrate (1), and vacuum-sputter a 20nm pure gold electrode layer (2) on the insulating substrate, and engrave the working electrode (3) and the reference electrode on the pure gold electrode by laser engraving technology. An electrode system such as an electrode (4), a sample introduction detection electrode (5) and a connecting electrode (6).

[0063]Preparation of basic buffer: prepare ACES buffer solution with a concentration of 0.2M and a pH v...

Embodiment 3

[0071] Example 3: Performance comparison between using oxidation current as detection signal and reduction current as detection signal

[0072] This example is a comparison of the performance of the uric acid test strips with the oxidation current as the detection signal and the reduction current as the detection signal.

[0073] The preparation method of the uric acid test paper using the reduction current as the detection signal in the present embodiment is as follows:

[0074] Preparation of the metal electrode: take the polymer insulating substrate (1), and vacuum-sputter a 20nm pure gold electrode layer (2) on the insulating substrate, and engrave the working electrode (3) and the reference electrode on the pure gold electrode by laser engraving technology. An electrode system such as an electrode (4), a sample introduction detection electrode (5) and a connecting electrode (6). Preparation of basic buffer: prepare ACES buffer solution with a concentration of 0.2M and a ...

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Abstract

According to the anti-interference electrochemical uric acid test paper and the preparation method thereof disclosed by the invention, compared with the prior art, the anti-interference electrochemical uric acid test paper is characterized in that an anti-interference area is ingeniously designed at an inlet of a siphon pool of a reaction window, and blood firstly flows through the anti-interference area containing ascorbic acid oxidase in the siphon pool through siphoning; ascorbic acid in a blood sample passing through the anti-interference area rapidly reacts with ascorbic acid oxidase, interference of ascorbic acid is eliminated, then blood flows into the reaction area to react with urate oxidase and a water-soluble electronic mediator, and the accuracy of determining different uric acid of different individuals is improved. Meanwhile, the metal electrode is combined, electron transfer is accelerated, current is reduced into a detection signal, detection can be carried out at a low negative potential, background current is further reduced, sensitivity is improved, and the detection time can be shortened to be within 10 seconds. By combining with the interference-removing enzyme layer, the ascorbic acid removing effect is remarkable, the sensitivity is high, and the accuracy is good.

Description

technical field [0001] The invention belongs to the technical field of medical equipment, and in particular relates to an anti-interference electrochemical uric acid test paper and a preparation method thereof. Background technique [0002] Uric acid is the end product of purine metabolism, and uric acid basically exists in the blood in the free form of monosodium uric acid in the body. Under a normal purine diet and two consecutive fasting tests on different days, a male > 416 μmol / L (7 mg / dl) and a female > 357 μmol / L (6 mg / dl) can be judged as hyperuricemia. Long-term hyperuricemia, the greater the possibility of gout in the next 5 years. Uric acid is closely related to kidney disease, and uric acid can directly cause microangiopathy in glomerular afferent arterioles, leading to chronic kidney disease. [0003] With the improvement of people's living standards, the intake of high-purine diets increases, long-term high-fat and high-protein diets, coupled with stres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3271G01N27/3275
Inventor 葛艳秋黄晶秦玉
Owner NANJING EAGLENOS CO LTD
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