Multifunctional microfluidic detection chip

Abstract

The invention discloses a multifunctional micro-fluidic detection chip. The detection chip comprises a chip body, wherein a sample injection cavity, a sample quantifying cavity, a sample overflow cavity, a diluent storage cavity, a diluent quantifying cavity, a diluent overflow cavity, a quantitative uniform mixing cavity, a reaction cavity and an air hole are formed in the chip body; a to-be-detected sample is injected into the sample injection cavity and enters the sample quantifying cavity through a micro-channel, and redundant samples enter the sample overflow cavity; diluent in the diluent storage cavity enters the diluent quantifying cavity through the micro-channel, and redundant diluent enters the diluent overflow cavity; the reaction cavity comprises one or more reaction chambersand a sample blank chamber; and after the sample in the sample quantifying cavity and the diluent in the diluent quantifying cavity are uniformly mixed in the quantitative uniform mixing cavity, the obtained mixture enters the reaction chamber through the micro-channel for a reaction with a reaction reagent to be detected, and meanwhile, the mixture enters the sample blank chamber to be used as asample blank for detection. The multifunctional micro-fluidic detection chip can effectively reduce sample consumption, improve the accuracy of detection results, and detect multiple indexes at the same time.

Description

technical field [0001] The invention belongs to the field of in vitro microfluidic detection, in particular to a multifunctional microfluidic detection chip. Background technique [0002] Microfluidic detection chip technology integrates the basic operation units of sample preparation, reaction, separation, and detection in the process of biological, chemical, and medical analysis into a micron-scale chip, and automatically completes the entire analysis process. It has great potential in the fields of biology, chemistry and medicine. [0003] However, the microfluidic detection chip technology in the prior art still has some defects in practical application, such as complex structure, large sample usage, inaccurate detection results, too small volume ratio of diluent quantification chamber and sample quantification chamber, production High cost etc. At present, the centrifugal microfluidic detection chip on the market requires a large amount of blood sample, which is not s...

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Problems solved by technology

[0003] However, the microfluidic detection chip technology in the prior art still has some defects in practical application, such as complex structure, large sample usage, inaccurate detection results, too small volume ratio of diluent quantification chamber and sample quantification chamber, production high cost

At present, the centrifugal microfluidic detection chip on the market requires a large amount of blood sample, which is not suitable for clinical blood collection detection of infants, especially newborns, and critically ill patients, and lacks effective quality control functions; so it is necessary to develop a centrifugal microfluidic chip. The detection chip has a simple structure, a small amount of samples can be injected at a time, and multiple indicators can be detected in a small volume. The production cost is low, suitable for mass production, and the detection results are more accurate. At the same time, multiple samples can be detected after chip assembly

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