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A method for culturing Bacillus subtilis by using Jinggangmycin plate and frame filter residues

A technology of Bacillus subtilis and Jinggangmycin, applied in the field of microbial cultivation, can solve problems such as food consumption and achieve the effect of promoting reproduction

Active Publication Date: 2022-07-15
ZHEJIANG TONGLU HUIFENG BIOSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Starch, peanut cake, etc. are used as nutrients in the cultivation of Bacillus subtilis, and there is a problem of food consumption. Today, when the country attaches great importance to food security, there is an urgent need to find a method for cultivating Bacillus subtilis that reduces food consumption.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take Jinggangmycin plate and frame filter residue, the mass percentage of Jinggangmycin is 0.2%, mix 1 part of Jinggangmycin plate and frame filter residue and 3 parts of tap water (W / V, g / mL), adjust with sodium hydroxide The pH value of the mixture was 13, heated to 80° C., kept for 1 hour, and the pH value was adjusted to 6.0 with sulfuric acid to obtain a hydrolyzed solution of Jinggangmycin plate and frame filter residues.

[0035] (1) Recipe per liter of fermentation medium: starch 30 g, peanut cake powder 20 g, potassium dihydrogen phosphate 1.5 g, dipotassium hydrogen phosphate 3 g, sodium chloride 1 g, magnesium sulfate 0.5 g, Jinggangmycin plate and frame filter residue hydrolyzate 60g, pH 7.0.

[0036] (2) Sterilization: Sterilize at 121°C for 30 minutes.

[0037] (3) Inoculation: when the fermentation medium is cooled to 36° C., inoculate with Bacillus subtilis, and ferment and culture.

[0038] (4) Fermentation culture: the temperature is 36°C, the ventil...

Embodiment 2

[0042] Take Jinggangmycin plate and frame filter residue, the mass percentage of Jinggangmycin is 2%, mix 1 part of Jinggangmycin plate and frame filter residue and 2 parts of tap water in the ratio (W / V, g / mL), use hydrogen peroxide The pH value of the mixture was adjusted to 12 with potassium, heated to 85° C., kept for 2 hours, and the pH value was adjusted to 7.0 with hydrochloric acid to obtain a hydrolyzed solution of Jinggangmycin plate and frame filter residues.

[0043](1) Fermentation medium formula per liter: starch 10g, peanut cake powder 30g, potassium dihydrogen phosphate 2g, dipotassium hydrogen phosphate 4g, sodium chloride 2g, magnesium sulfate 1g, Jinggangmycin plate and frame filter residue hydrolyzate 150g, pH The value is 7.5.

[0044] (2) Sterilization: 121 ℃, sterilization 30min.

[0045] (3) Inoculation: when the fermentation medium is cooled to 38° C., inoculate with Bacillus subtilis, and ferment and culture.

[0046] (4) Fermentation culture: the t...

Embodiment 3

[0049] Take Jinggangmycin plate and frame filter residue, the mass percentage of Jinggangmycin is 4%, mix 1 part of Jinggangmycin plate and frame filter residue and 4 parts of tap water in the ratio (W / V, g / mL), use hydrogen peroxide The pH value of the mixture was adjusted to 12 by sodium, heated to 82° C., kept for 2 hours, and the pH value was adjusted to 8.0 with phosphoric acid to obtain a hydrolyzed solution of Jinggangmycin plate and frame filter residues.

[0050] (1) Fermentation medium formula: starch 50g, peanut cake powder 10g, potassium dihydrogen phosphate 3g, dipotassium hydrogen phosphate 6g, sodium chloride 3g, magnesium sulfate 1.5g, Jinggangmycin plate and frame filter residue hydrolyzate 45g, pH value is 8.

[0051] (2) Sterilization: 121 ℃, sterilization 30min.

[0052] (3) Inoculation: when the fermentation broth is cooled to 37° C., inoculate with Bacillus subtilis, and ferment and culture.

[0053] (4) Fermentation culture: the temperature is 37°C, th...

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PUM

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Abstract

The invention provides a method for culturing Bacillus subtilis by using Jinggangmycin plate and frame filter residues, belonging to the technical field of microorganism culture, comprising the following steps: 1) mixing Jinggangmycin plate and frame filter residues with water and an alkali reagent to obtain a mixture, and mixing The mixture is subjected to alkaline hydrolysis to obtain an alkaline hydrolysis solution, and the pH value of the alkaline hydrolysis solution is adjusted to 6-8 to obtain a hydrolyzed solution of Jinggangmycin plate and frame filter residue; 2) the Jinggangmycin plate and frame filter residue obtained in the step 1). The hydrolyzed liquid is mixed with fermentation medium and Bacillus subtilis for fermentation, and Bacillus subtilis is collected; the fermentation medium uses water as a solvent, and each liter contains 10-50 g of starch and 10-30 g of peanut cake powder. The content of Bacillus subtilis obtained by the method provided by the invention is higher, the number of spores per unit is increased by more than 50% compared with the original method, and some raw materials are replaced by Jinggangmycin plate and frame filter residues instead of part of the starch and peanut cake powder raw materials, which reduces the production cost. .

Description

technical field [0001] The invention belongs to the technical field of microorganism culture, in particular to a method for culturing Bacillus subtilis by using Jinggangmycin plate and frame filter residues. Background technique [0002] Jinggangmycin is a biological pesticide, which is prepared by microbial fermentation process. The Jinggangmycin fermentation process uses raw materials such as rice and peanut cakes, which are fermented by microorganisms to produce fermentation liquid, and the active ingredients are extracted through plate and frame filtration, ion exchange resin, drying and other processes. Frame filtration produces plate and frame filtration residue, which is the main source of solid waste in the fermentation process. [0003] In the National Hazardous Waste List (2019), 263-010-04 waste filter materials and adsorbents generated during pesticide production in HW04 pesticide waste are classified as hazardous waste, while Jinggangmycin is a biological pestic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/125
CPCC12N1/20
Inventor 殷红福李忠吴闯倪烈
Owner ZHEJIANG TONGLU HUIFENG BIOSCI