New application of scutellaria baicalensis flavonoid compounds and/or metal ion complexing agents in enhancement of polymyxin antibiotic antibacterial effect

A polymyxin and metal ion technology, applied in the field of medicine, can solve problems such as weak antibacterial activity, and achieve the effects of reducing toxic and side effects, treating bacterial infections, and reducing the dosage of medicines

Active Publication Date: 2021-01-22
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that the flavonoids of Scutellaria baicalensis have inhibitory effect on bacteria, but their antibacterial activity is weak compared with antibiotics or synthetic antibacterial drugs

Method used

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  • New application of scutellaria baicalensis flavonoid compounds and/or metal ion complexing agents in enhancement of polymyxin antibiotic antibacterial effect
  • New application of scutellaria baicalensis flavonoid compounds and/or metal ion complexing agents in enhancement of polymyxin antibiotic antibacterial effect
  • New application of scutellaria baicalensis flavonoid compounds and/or metal ion complexing agents in enhancement of polymyxin antibiotic antibacterial effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The antibacterial effect test of colistin (polymyxin E) of polymyxins, baicalin, a flavonoid compound of baicalein, and metal ion complexing agent EDTA-2Na against Salmonella clinical isolate SH01, materials and methods used Proceed as follows:

[0016] 1. Test drug:

[0017] Baicalin: produced by Chengdu Ruifensi Biotechnology Co., Ltd., content: >98%. Colistin: produced by Hebei Shengxue Dacheng Tangshan Pharmaceutical Co., Ltd., potency ≥ 23000 u / mg. EDTA-2Na: produced by Sinopharm Chemical Reagent Co., Ltd.

[0018] Baicalin was formulated with deionized water to a concentration of 5000 μg / mL, and 0.9 wt% sodium carbonate solution was added to aid in dissolution. EDTA-2Na was prepared to a concentration of 5000 μg / mL with deionized water. Colistin was prepared to a concentration of 5120 μg / mL with deionized water. Test drugs were stored at -20°C. Before the test, the drug stock solution was taken out and thawed at room temperature, mixed well, and the pharmac...

Embodiment 2

[0044] The antibacterial effect test of colistin (polymyxin E) of polymyxins, baicalin, a baicalin flavonoid compound, and metal ion complexing agent EDTA-2Na, on clinically isolated Salmonella SH02, materials and method steps used as follows:

[0045] 1. Test drug:

[0046] With embodiment 1.

[0047] 2. strain

[0048] Clinically isolated Salmonella strains: Salmonella SH02 was provided by the Pharmacology Laboratory of Henan Agricultural University, and all were identified by PCR and mass spectrometry (MALDI-TOF / MS). Salmonella was activated on SS medium, cultured at 37°C for 16 h, and stored at 4°C for later use.

[0049] 3. culture medium

[0050] With embodiment 1.

[0051] 4. instrument

[0052] With embodiment 1.

[0053] 5. Single-drug susceptibility by broth microdilution

[0054] With embodiment 1.

[0055] 6. Determination of single-drug susceptibility MIC value

[0056] With embodiment 1.

[0057] 7. Broth microdilution combined with susceptibili...

Embodiment 3

[0068] The antibacterial effect test of colistin (polymyxin E) of polymyxins, baicalin, a flavonoid compound of baicalein, and metal ion complexing agent EDTA-2Na, against Salmonella clinical isolate SH03, materials and methods used Proceed as follows:

[0069] 1. Test drug:

[0070] With embodiment 1.

[0071] 2. strain

[0072] Clinically isolated Salmonella strains: Salmonella SH03 was provided by the Pharmacology Laboratory of Henan Agricultural University, and all were identified by PCR and mass spectrometry (MALDI-TOF / MS). Salmonella was activated on SS medium, cultured at 37°C for 16 hours, and stored at 4°C for later use.

[0073] 3. culture medium

[0074] With embodiment 1.

[0075] 4. instrument

[0076] With embodiment 1.

[0077] 5. Single-drug susceptibility by broth microdilution

[0078] With embodiment 1.

[0079] 6. Determination of single-drug susceptibility MIC value

[0080] With embodiment 1.

[0081] 7. Broth microdilution combined with...

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Abstract

The invention belongs to the technical field of medicines, and particularly relates to a new application of scutellaria baicalensis flavonoid compounds and / or metal ion complexing agents in enhancement of polymyxin antibiotic antibacterial effect. In-vitro tests prove that the scutellaria baicalensis flavonoid compounds and the metal ion complexing agents are combined with polymyxin antibacterialagents, so that the antibacterial activity of polymyxin antibiotics can be improved to different degrees, and the drug resistance of bacteria to the polypeptide antibiotics can be reversed. Therefore,a synergist of the polymyxin antibiotics can be used, and used for preparing a compound preparation. Under the conditions that the drug resistance of the clinical polymyxin antibiotics is increasingly common and the drug resistance degree is increasingly serious, the new application can recover the sensitivity of the drug-resistant bacteria to the polymyxin antibiotics, can reduce the dosage of the polymyxin antibiotics such as the colistin, improve the antibacterial treatment effect, and reduce the toxic and side effects, and has significant clinical application value.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the new use of baicalin flavonoids and / or metal ion complexing agents in synergizing the antibacterial effect of polymyxin antibiotics. Background technique [0002] Polymyxin antibiotics (often abbreviated as polymyxins) are cationic polypeptides with broad-spectrum anti-negative bacteria, mainly through the polycation ring in the molecule and the lipids on the bacterial outer membrane lipopolysaccharides (lipopolysaccharides LPS). The A function replaces cations such as calcium and magnesium in the outer membrane, destroys the negatively charged outer membrane of negative bacteria and reduces the stability of the cell membrane, increases the permeability of the outer membrane of the cell, and causes the important substances in the cell to flow out to produce a bactericidal effect. According to their different chemical structures, polymyxins can be divided into 8 ki...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/12A61P31/04A61K31/7048A61K31/352A61K33/42A61K31/198
CPCA61K38/12A61K31/7048A61K31/352A61K33/42A61K31/198A61P31/04A61K2300/00Y02A50/30
Inventor 胡功政崔小蝶吴华贺丹丹翟亚军
Owner HENAN AGRICULTURAL UNIVERSITY
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