Molecular marker for identifying Clupanodon thrissa and Konosirus punctatus based on mitochondrial COI gene and application of the molecular marker

Abstract

The invention belongs to the technical field of biology, and particularly relates to a molecular marker for identifying Clupanodon thrissa and Konosirus punctatus based on a mitochondrial COI gene andapplication of the molecular marker. The Konosirus punctatus is widely distributed in coastal areas of China, and coastal areas of Korea and Japan. The Clupanodon thrissa is mainly distributed in east China sea, south China sea and coastal areas of Korea. Distribution areas of the two kinds of fishes are overlapped, the appearances of the two kinds of fishes are very similar, and the two kinds offishes are offshore economic fishes in China and are difficult to quickly identify from the appearances. A pair of specific primers (HB3) is designed and screened by comparing and analyzing specificloci of the COI gene in mtDNA. The specific primers HB3 can be amplified in the Clupanodon thrissa to obtain a single product of 537bp, and no product exists in the Konosirus punctatus. Two species can be distinguished by presence or absence of a PCR amplification product, and are verified in 30 randomly selected samples. The invention provides a method capable of quickly identifying the Clupanodon thrissa and the Konosirus punctatus without sequencing.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker for distinguishing chrysalis and spotted chrysalis based on the mitochondrial COI gene and its application. Background technique [0002] Catfish, belonging to the order Herringomorpha, Herringidae, Subfamily Catfish, and the genus Catfish, is widely distributed in the coastal areas of my country, as well as the coasts of South Korea and Japan. The chrysalis belongs to the order Herringoformes, the family Herringae, the subfamily of the genus, and is mainly distributed in the East my country Sea, the South China Sea and the coast of South Korea. Both the grouped catfish and the blue catfish are economic fishes in the coastal waters of my country. The distribution areas of the two fishes overlap, and their shapes are very similar, and it is difficult to quickly distinguish them from their shapes (especially after being in the water for a long time). Cu...

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Problems solved by technology

Currently, no studies have been reported on the use of molecular markers to differentiate the two species of fish

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