Base interpretation method based on nanopore electric signal, interpretation equipment and storage medium

Abstract

The invention discloses a base interpretation method based on a solid nanopore electric signal, interpretation equipment and a storage medium. The method comprises the steps: constructing a base interpretation model through big data training; interpreting the electric signal data into a base sequence of DNA or cDNA according to the base interpretation model; and generating a sequencing data resultfile in a standard format from the base sequence. Through the embodiment of the invention, independent sequencing of each DNA molecule can be realized, PCR (Polymerase Chain Reaction) amplification is not needed, and meanwhile, the method has higher data reading speed and longer sequencing sequence reading length, and has the advantages of high flux, low cost and long reading length.

Description

Technical field[0001]The invention relates to the field of gene detection, in particular to a base interpretation method, interpretation equipment and storage medium based on solid-state nanopore electrical signals.Background technique[0002]Efficient and convenient interpretation of genetic information contained in organisms is of great significance to the fields of life sciences and precision medicine. Since 1952, Hershey and Chase proved that the genetic information is transmitted by DNA sequence, the research around DNA sequencing technology has always been a hot spot and core in the field of life sciences.[0003]At present, the technical principle of second-generation sequencing is to break the sequence to be tested into small fragments with a length of 200-500 bps, and add different adapters at both ends of these small fragments, which depends on a large amount of PCR amplified DNA The small fragment template is combined with the solid surface, and then the DNA polymerase, adapt...

AI Technical Summary

Problems solved by technology

However, the sequence read length of next-generation sequencing is relatively short, only 150-300bps, which greatly limits its application in the analysis and research of copy number variation, structural variation, haplotype analysis, and genome assembly.

At the same time, since the signal of next-generation sequencing is a fluorescent signal, in order to reach the lower limit threshold of fluorescent signal detection, PCR amplification must be performed, and single-molecule sequencing cannot be realized.

Images