Preserving fluid suitable for preserving virus samples at normal temperature and preparation method thereof

Abstract

The invention discloses a preserving fluid suitable for preserving virus samples at normal temperature and a preparation method thereof. The preserving fluid comprises 150-250 g of sodium chloride, 8-12 g of potassium chloride, 20-30 g of D-glucose, 3-5 g of sodium hydroxide, 0.4-0.6 g of phenol red, 2-4 g of dipotassium hydrogen phosphate, 1-1.5 g of disodium hydrogen phosphate, 8-9 g of sodium bicarbonate, 4-5 g of bovine serum albumin, 0.5-0.9 g of 2-methyl-4-isothiazoline-3-ketone hydrochloride and 0.1-0.5 g of polymyxin B sulfate. The invention relates to the technical field of clinical examination. According to the preserving fluid suitable for preserving virus samples at normal temperature and the preparation method thereof, the prepared preserving fluid has good preserving capability, can ensure that a sample virus still has activity after preservation for more than one week at normal temperature and can be preserved at 2-8 DEG C for a longer time, does not interfere a PCR detection result, has good antibacterial capability, has low requirements on production and use environments, does not need irradiation sterilization, is low in cost and easy to mass-produce, guarantees no decomposition of nutrients, and guarantees the stability of the pH value in cooperation with an HEPES buffer solution or a phosphate buffer solution.

Description

technical field [0001] The invention relates to the technical field of clinical testing, in particular to a preservation solution suitable for storing virus samples at room temperature and a preparation method thereof. Background technique [0002] Virus infection refers to the process in which viruses invade the body through various channels and proliferate in susceptible host cells. Human viruses refer to viruses that can infect the human body or have pathogenic effects on humans. The essence of virus infection is the process of interaction between virus and body, virus and susceptible cells. Virus infection often produces varying severity of injuries or viral diseases due to different virus types and body states. Viruses cause disease by invading the host and infecting cells. The pathogenic effect is manifested in two aspects of the human body and cells. After the body is infected with the virus, it can show different clinical types. According to the presence or absence ...

AI Technical Summary

Problems solved by technology

Viruses cause disease by invading the host and infecting cells. The pathogenic effect is manifested in two aspects of the human body and cells. After the body is infected with the virus, it can show different clinical types. According to the presence or absence of symptoms, it can be divided into dominant infection and recessive infection; according to the residence time of the virus and the duration of symptoms, it can be divided into acute infection and persistent infection. In order to comprehensively analyze the cause of viral infection, it is often necessary to isolate the virus from the patient and identify it through analysis , to determine the type of virus infection, the live virus can maintain the originality of the sample to the greatest extent, it can be used for the cultivation and isolation of the virus, and it can also be used for various molecular biological detection of the virus. However, due to transportation, cold chain, storage and other factors, the storage time of Hanks solution is short, and the integrity of virus morphology is easily affected, which is not conducive to the isolation and identification of viruses, and is also not conducive to the extraction rate of viruses, and it is easy to breed bacteria, Fungi, causing the pH value of Hanks solution to drop. When the pH value of Hanks solution is less than 7.4, the stability of the virus decreases, the decomposition speed of the virus is accelerated, and it is difficult to preserve the virus activity for a long time

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