Method for obtaining larch regeneration plants through tissue in-vitro culture

A technology for regenerating plants and in vitro culture, which is applied in the fields of agriculture, forestry, science and technology and biology. It can solve the problems of complex genetic background and long growth cycle of larch, achieve good air permeability, promote germination, and efficiently induce callus formation and proliferation. Effect

Inactive Publication Date: 2021-02-09
TIANJIN AGRICULTURE COLLEGE
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Problems solved by technology

Crossbreeding is the main method to obtain excellent strains of larch, but larch has a long growth cycle and complex genetic background, it is difficult to obtain homozygous parents in a short period of time like crossbreeding of crops, and through crossbreeding to obtain Heterozygous offspring with heterosis and uniform traits

Method used

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  • Method for obtaining larch regeneration plants through tissue in-vitro culture
  • Method for obtaining larch regeneration plants through tissue in-vitro culture
  • Method for obtaining larch regeneration plants through tissue in-vitro culture

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Embodiment 1

[0029] Mature larch seeds were purchased from the National Larix Fine Variety Forest Farm Seed Garden in Qingyuan County, Fushun City, Liaoning Province. After retrieval, they were placed in a refrigerator at 4°C for 14 days, and then mature and plump seeds were selected and soaked at room temperature for 48 hours (the soaking solution contained 400mg / L gibberellin and 2% cellulase), and then washed three times with tap water. Sow the treated and washed larch seeds in the sterilized river sand, then cover the larch seeds with a layer of 0.5cm thick sand, keep the sand moist, and cultivate them in a cultivation room at a temperature of 22-23°C for 30 days to harvest Larch seedling material.

[0030] Seedlings grow to about 25 days, select well-grown seedlings, and harvest the aboveground parts of the seedlings. Wash the seedlings with 5% sodium hypochlorite solution for 6 min, 2 min each time, 3 times in total; wash 3 times with sterile water, 2 min each time; disinfect with 7...

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Abstract

The invention relates to a method for obtaining larch regeneration plants through tissue in-vitro culture. The method comprises the following steps of culturing a larch material; obtaining and disinfecting a larch in-vitro tissue; carrying out dedifferentiation and multiplication culture on the larch in-vitro tissue; conducting adventitious bud induction and growth; conducting adventitious root induction and proliferation; and hardening seedlings of the larch regeneration plants, transplanting the seedlings and the like. According to the method, totipotency of plant cells is fully utilized tobe combined with a tissue culture technology, the larch regeneration plants can be obtained rapidly and efficiently in a large scale without season limitation, rapid and large-scale breeding of excellent larch strains is achieved, the afforestation problem that the seed breeding period is long and characters are unstable is solved, and an important guarantee breeding strategy is provided for larchto better serve economic construction and environmental protection.

Description

technical field [0001] The invention relates to a method for obtaining regenerated plants through in vitro culture of larch tissue. The invention can realize rapid and large-scale breeding of larch fine strains, and overcome the afforestation problem of long larch seed breeding time. At the same time, the present invention also provides a genetic transformation system for obtaining transgenic or gene-edited larch by means of genetic engineering. The invention belongs to the fields of agriculture and forestry science and technology and biotechnology. Background technique [0002] Larix is ​​an important timber and greening tree species, which has strong adaptability, strong corrosion resistance and ecological protection function. Therefore, larch has dual value of economy and environment, and is widely planted in our country and around the world. Crossbreeding is the main method to obtain excellent strains of larch, but larch has a long growth cycle and complex genetic bac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02P60/40Y02A40/22
Inventor 李慧王春国陈成彬
Owner TIANJIN AGRICULTURE COLLEGE
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