Application of ribosome sigma factor, mutant thereof and protein obtained by encoding in increasing yield of lipstatin

Abstract

The invention discloses application of a ribosome sigma factor, a mutant thereof and protein obtained by encoding in increasing the yield of lipstatin. The ribosome sigma factor is a ribosome sigma factor A or a ribosome sigma factor B, the nucleotide sequence of the ribosome sigma factor A is shown as SEQ ID NO.5, and the nucleotide sequence of the ribosome sigma factor B is as shown in SEQ ID NO. 7; and the mutants of the ribosome sigma factors A and B are further comprised, the nucleotide sequence of the mutant of the ribosome sigma factor A is shown as SEQ ID NO. 1, and the nucleotide sequence of the mutant of the ribosome sigma factor B is shown as SEQ ID NO. 3. According to the application, the overexpression of the ribosome sigma factors A and B obtained by screening and the mutantsof the ribosome sigma factors A and B can improve the yield of lipstatin.

Description

technical field

[0001] The invention belongs to the technical field of microbial genetic engineering, and specifically relates to a ribosomal sigma factor and its mutant and the application of the encoded protein in increasing the output of riprestatin. Background technique

[0002] Orlistat, the tetrahydro derivative of liprestatin, is currently certified by the US FDA, EU EMA and China SFDA as an obesity treatment drug that does not suppress appetite and does not act on the central nervous system. More than 40 million people around the world take it And successfully lose weight, it is currently the best-selling weight loss product. As a long-acting specific pancreatic lipase inhibitor, it can prevent the hydrolysis of triglycerides into free fatty acids and monoacylglycerols that can be absorbed by the small intestinal mucosa, thereby reducing calorie intake and controlling body weight. And liprestatin was originally isolated from Streptomyces toxytricini. It has a β-prop...

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