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Disease inoculation method for achnatherum inebrians ergot disease

A technology of drunk horse grass and ergot, applied in the field of microbial inoculation, can solve the problems of low spore concentration, large impact, and difficult disease, and achieve the effect of simple method, good moisturizing effect, and high incidence rate

Pending Publication Date: 2021-02-12
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Yet the inoculation method of existing ergotism is the injection inoculation method, and the disease inoculation method is loaded down with trivial details, and is affected by weather after inoculation disease is bigger, if meet cloudy and rainy weather, the spore of injection is easily washed away by rainwater, is difficult to come on, injection The concentration of spores is lower, and the infection rate after inoculation is lower

Method used

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  • Disease inoculation method for achnatherum inebrians ergot disease
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  • Disease inoculation method for achnatherum inebrians ergot disease

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Embodiment 1

[0029] This embodiment provides a kind of inoculation method of drunk horse grass ergot disease, it comprises the following steps:

[0030] S1. Harvest the seeds of Drunken Horsegrass stored at 4°C in the storage room of the Forage Grass and Turfgrass Seed Quality Supervision, Inspection and Testing Center (Lanzhou) of the Ministry of Agriculture, and sow them in the grid test field of Lanzhou University Yuzhong Campus.

[0031] S2, the drunken horse grass ergot collected from Xinjiang, Gansu and Qinghai is cut into 4mm segments with a sterile knife, inoculated onto the potato dextrose agar medium (PDA) with sterile tweezers respectively, sealed with a parafilm, and placed In a constant temperature incubator at 25°C, cultivate for 4 days, and after white hyphae grow out, transfer the fungus to PDA medium for purification, and obtain the purified strain.

[0032] S3. Inoculate the purified strains from the above three regions on the PDA medium in the form of bacterium cake, put...

Embodiment 2

[0037] This embodiment provides a kind of inoculation method of drunk horse grass ergot disease, it comprises the following steps:

[0038] S1. Harvest the seeds of Drunken Horsegrass stored at 4°C in the storage room of the Forage Grass and Turfgrass Seed Quality Supervision, Inspection and Testing Center (Lanzhou) of the Ministry of Agriculture, and sow them in the grid test field of Lanzhou University Yuzhong Campus.

[0039] S2, the drunken horse grass ergot collected from Xinjiang, Gansu and Qinghai is cut into 3 mm segments with a sterile knife, inoculated onto the potato dextrose agar medium (PDA) with sterile tweezers respectively, sealed with a parafilm, and placed In a constant temperature incubator at 23°C, cultivate for 3 days, and after the growth of white hyphae, transfer the fungus to PDA medium for purification, and obtain the purified strain.

[0040] S3. Inoculate the purified strains from the above three regions on the PDA medium in the form of bacterium cak...

Embodiment 3

[0045] This embodiment provides a kind of inoculation method of drunk horse grass ergot disease, it comprises the following steps:

[0046] S1. Harvest the seeds of Drunken Horsegrass stored at 4°C in the storage room of the Forage Grass and Turfgrass Seed Quality Supervision, Inspection and Testing Center (Lanzhou) of the Ministry of Agriculture, and sow them in the grid test field of Lanzhou University Yuzhong Campus.

[0047]S2, the drunken horse grass ergot collected from Xinjiang, Gansu, Qinghai is cut into 5 mm segments with a sterile knife, inoculated onto the potato dextrose agar medium (PDA) with sterile tweezers respectively, sealed with a parafilm, and placed In a constant temperature incubator at 27°C, culture for 5 days, and after white hyphae grow, transfer the fungus to PDA medium for purification, and obtain the purified strain.

[0048] S3. Inoculate the purified strains from the above three regions on the PDA medium in the form of bacterium cake, put them in ...

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Abstract

The invention is suitable for the technical field of microbial inoculation, and provides a disease inoculation method for an achnatherum inebrians ergot disease. The method comprises the following steps that spore suspension is prepared from achnatherum inebrians claviceps so as to inoculate achnatherum inebrians; in the initial flowering stage of the achnatherum inebrians, water is sprayed to theears of the achnatherum inebrians, then spore suspension is sprayed to the ears of the achnatherum inebrians, and the ears of the sprayed claviceps are sleeved with transparent ziplock bags; and thetransparent ziplock bags are taken off, and the spore suspension is repeatedly sprayed during the blooming period of the achnatherum inebrians. According to the method, the spore suspension is adopted, disease inoculation is conducted through a spraying method, the method is simple, and the morbidity after inoculation is high; the transparent ziplock bag sleeved after disease inoculation has a good moisturizing effect, is not influenced by cloudy and rainy weather, and is suitable for large-batch production of the ergos; and besides, the selected host plant is achnatherum inebrians, the endophytic fungi in the achnatherum inebrians can generate alkaloid, and rich alkaloid can also be generated through infection of claviceps, so that a good raw material is provided for extraction of alkaloid.

Description

technical field [0001] The invention belongs to the technical field of microbial inoculation, in particular to a method for inoculating Drunken Horse Grass ergot disease. Background technique [0002] Drunken horse grass (Achnatherumine brians) is one of the main potent poisonous weeds in the natural grasslands of northern my country. It is mainly distributed in the natural grasslands of northern and northwestern my country and Mongolia, and is poisonous to sheep, cattle, horses and other livestock. The researchers found that five or six provinces (districts) such as Xinjiang, Qinghai, Gansu, Inner Mongolia, Ningxia and Tibet had a bacterial carrier rate of more than 95%, some as high as 100%. [0003] The alkaloid toxin produced by Claviceps is easy to cause poisoning to humans and animals, and also has a variety of biological activities, and has been widely used in medicine. As early as 1474, the German prescription has recorded medicinal use, and it is mainly used in med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06C12N1/14C12N3/00C12R1/645
CPCA01G7/06C12N1/14C12N3/00
Inventor 李春杰张海娟何雅丽
Owner LANZHOU UNIVERSITY
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