Multi-object-plane simultaneous imaging method based on digital multiplexing lens

Abstract

The invention provides a multi-object-plane simultaneous imaging method based on a digital multiplexing lens. A digital multiplexing lens programmed and modulated by a liquid crystal spatial light modulator is combined with an axial multi-object-plane simultaneous imaging system formed by an infinite tube length microscope objective front group with a fixed focal length, so that any cross sectioncan be clearly imaged or multiple cross sections can be clearly imaged at the same time within a certain depth range of a sample, and an image surface with diffraction image limiting quality is formed, which deviates in the axial direction and the transverse direction at the same time, on the same imaging plane. The number of imaging surfaces, the axial interval and the transverse offset distancebetween the image surfaces of the system can be controlled and adjusted in real time through programming. The multi-object-plane simultaneous imaging method based on a digital multiplexing lens does not need axial scanning, has time and space resolution, is flexible, and has important practical application value in the fields of biomedical imaging, pharmacology, material science and the like.

Description

technical field [0001] The invention belongs to the field of optical imaging, and in particular relates to a multi-object plane simultaneous imaging method based on a digital multiplexing lens. Background technique [0002] Optical imaging technology, as a direct visualization tool for studying life phenomena in living cells, has always been a major challenge to achieve flexible temporal and spatial resolution imaging. Existing imaging technologies, including laser confocal scanning microscope, two-photon laser scanning microscope, multi-focus multi-photon microscopic imaging technology, optical coherence tomography, etc., need to scan and image the sample laterally and axially, which limits imaging speed, the time-resolved property is lost. [0003] Although the multi-focal plane parallel imaging method based on deformed grating can realize multi-section simultaneous imaging, its section imaging ability is mainly concentrated in the lower diffraction order 0 order and ±1 o...

AI Technical Summary

Problems solved by technology

Existing imaging technologies, including laser confocal scanning microscope, two-photon laser scanning microscope, multi-focus multi-photon microscopic imaging technology, optical coherence tomography, etc., need to scan and image the sample laterally and axially, which limits imaging time resolution

[0003] The multi-focal plane parallel imaging method based on deformed grating can realize multi-section simultaneous imaging, but its cross-section imaging ability is mainly concentrated in the lower diffraction order 0 order and ±1 order, and the diffraction energy of each order is not uniform

Based on twisted Damman grating and multi-object surface simultaneous imaging system, the problem of uneven energy distribution of cross-sectional imaging is solved, and more cross-sectional imaging is realized, but it involves complex processing technology and detection technology, and it is flexible and real-time Not enough, when changing the number of sections and section spacing, it is necessary to reprocess the twisted Damman grating

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