A Simultaneous Imaging Method of Multiple Object Surfaces Based on Digital Multiplexing Lens

Abstract

The invention proposes a multi-object plane simultaneous imaging method based on a digital multiplexing lens. The digital multiplexing lens programmed and modulated by the liquid crystal spatial light modulator, combined with the fixed focal length infinite barrel length microscope objective lens front group constitutes an axial multi-object surface simultaneous imaging system, which can image any cross-section within a certain depth range of the sample Clear, or multiple cross-sections are imaged at the same time, and an image plane with diffraction-limited image quality that is shifted along the axial and lateral directions at the same time is formed on the same imaging plane. The number of imaging planes, the axial spacing and the lateral offset distance between the imaging planes of the system can be controlled and adjusted in real time through programming. The invention can realize a flexible three-dimensional imaging system that does not require axial scanning, has both time and space resolution, and has important practical application value in the fields of biomedical imaging, pharmacology, material science and the like.

Description

technical field [0001] The invention belongs to the field of optical imaging, and in particular relates to a multi-object plane simultaneous imaging method based on a digital multiplexing lens. Background technique [0002] Optical imaging technology, as a direct visualization tool for studying life phenomena in living cells, has always been a major challenge to achieve flexible temporal and spatial resolution imaging. Existing imaging technologies, including laser confocal scanning microscope, two-photon laser scanning microscope, multi-focus multi-photon microscopic imaging technology, optical coherence tomography, etc., need to scan and image the sample laterally and axially, which limits imaging speed, the time-resolved property is lost. [0003] Although the multi-focal plane parallel imaging method based on deformed grating can realize multi-section simultaneous imaging, its section imaging ability is mainly concentrated in the lower diffraction order 0 order and ±1 o...

AI Technical Summary

Problems solved by technology

Existing imaging technologies, including laser confocal scanning microscope, two-photon laser scanning microscope, multi-focus multi-photon microscopic imaging technology, optical coherence tomography, etc., need to scan and image the sample laterally and axially, which limits imaging time resolution

[0003] The multi-focal plane parallel imaging method based on deformed grating can realize multi-section simultaneous imaging, but its cross-section imaging ability is mainly concentrated in the lower diffraction order 0 order and ±1 order, and the diffraction energy of each order is not uniform

Based on twisted Damman grating and multi-object surface simultaneous imaging system, the problem of uneven energy distribution of cross-sectional imaging is solved, and more cross-sectional imaging is realized, but it involves complex processing technology and detection technology, and it is flexible and real-time Not enough, when changing the number of sections and section spacing, it is necessary to reprocess the twisted Damman grating

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