Application of miR-325 nucleic acid analog in preparation of sinusoid endothelial cell pathologic dysfunction treatment related product

A 1. mir-325, nucleic acid analog technology, applied in the direction of vascular endothelial cells, animal cells, extracellular fluid diseases, etc., can solve the problems of difficulty in recovering normal liver function, irreversible liver collagen deposition, etc., to promote regulation Inflammation, low cost, great effect

Pending Publication Date: 2021-02-26
XIAN TISSUE ENG & REGENERATIVE MEDICINE RES INST
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Problems solved by technology

However, there is currently no specific drug for the treatment of patients with liver fibrosis or cirrhosis in clinical practice. The conventional regimens used can only target the cause of liver disease by anti-virus, etc. to slow down the process of liver collagen deposition, or to protect the li

Method used

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  • Application of miR-325 nucleic acid analog in preparation of sinusoid endothelial cell pathologic dysfunction treatment related product
  • Application of miR-325 nucleic acid analog in preparation of sinusoid endothelial cell pathologic dysfunction treatment related product
  • Application of miR-325 nucleic acid analog in preparation of sinusoid endothelial cell pathologic dysfunction treatment related product

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Experimental program
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Embodiment

[0052] 1. Detection of abnormal sinus function:

[0053] 1. Sinusoid primary cells were isolated and cultured for differentiation and characterization detection

[0054] Fresh individual biopsies were minced and treated with Liberase TM Dissolve in DMEM medium with 2% fetal bovine serum and digest for 1 hour, blow gently to form single cells, culture in DMEM medium containing 10% fetal bovine serum, change the medium after about 4 to 7 days according to the attachment condition, The primary sinusoidal endothelial cells adhered to the wall were obtained. After being cultured to a density suitable for photographing a single cell (about 20%), the cells in the culture dish were fixed, dehydrated, and sprayed with gold. Then, under a scanning electron microscope, it was detected whether the cell surface had lost the characteristic structure of differentiation - sieve plate and window hole. The result is as Figure 1A-1B shown.

[0055] Biopsies stained for markers of sinusoidal...

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Abstract

The invention provides application of a miR-325 nucleic acid analog in preparation of a sinusoid endothelial cell pathologic dysfunction treatment related product. Through recovering expression of genes of cells stabilin-2, PTPRM and YWHAQ by signal channels directly and indirectly influenced by miR-325, sinusoid endothelial cells can re-differentiate, the capacity of the sinusoid endothelial cells for regulating inflammations and resisting activation of stellate cells is promoted, and thus, vascular pathological changes can be effectively relieved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of miR-325 nucleic acid analogs in the preparation of products related to the treatment of pathological dysfunction of sinusoidal endothelial cells. Background technique [0002] Sinusoidal endothelial cells (SEC) are special capillary endothelial cells without basement membrane, with fenestrations and sieve plates. Liver sinusoidal endothelial cell (LSEC) mainly ultrafilters blood through the structure of fenestration and sieve plate to achieve rapid exchange of nutrients and substances to meet the metabolic needs of a healthy liver. Studies have confirmed that LSEC can inhibit the activation of hepatic stellate cells, thereby inhibiting the main source of collagen deposition in the liver. In addition, LSECs control the expansion of liver parenchymal cells by expressing, secreting, or responding to receptors and cytokines such as hepatocyte growth factor (HGF), vasc...

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Application Information

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IPC IPC(8): A61K31/7105A61K45/06A61K35/28A61P25/00A61P5/00A61P7/00A61P9/14A61P25/28A61P9/10A61P3/10A61P13/12A61P1/18A61P7/06A61P1/14A61P9/12A61P7/02A61P1/16C12Q1/6881C12N5/071
CPCA61K31/7105A61K45/06A61K35/28A61P25/00A61P5/00A61P7/00A61P9/14A61P25/28A61P9/10A61P3/10A61P13/12A61P1/18A61P7/06A61P1/14A61P9/12A61P7/02A61P1/16C12Q1/6881C12N5/069C12Q2600/158C12Q2600/178C12N2501/65
Inventor 李丽雅刘文佳
Owner XIAN TISSUE ENG & REGENERATIVE MEDICINE RES INST
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