Two-line hybrid wheat yield heterosis related protein TaHMT39 and encoding gene and application thereof

A hybrid vigor and related protein technology, applied in the field of genetic engineering, can solve the problems of limiting the breeding efficiency of two-line hybrid wheat in the industrialization process of molecular breeding, unclear regulatory pathways and key genes, and weak molecular mechanism research.

Active Publication Date: 2021-03-19
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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AI Technical Summary

Problems solved by technology

The fertility conversion of two-line hybrid wheat has obvious epigenetic characteristics. However, so far, the research on its molecular mechanism is still weak, and its regulatory pathways and key genes are still unclear, which limits the breeding of two-line hybrid wheat. Improvement of Efficiency and Industrialization Process of Molecular Breeding

Method used

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  • Two-line hybrid wheat yield heterosis related protein TaHMT39 and encoding gene and application thereof
  • Two-line hybrid wheat yield heterosis related protein TaHMT39 and encoding gene and application thereof
  • Two-line hybrid wheat yield heterosis related protein TaHMT39 and encoding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Young panicles and phenotype identification of strong dominant hybrids

[0040] Select the same male parent female parent and the first generation plants of the offspring, rinse with water, and quickly cut the tiller nodes of the root base and stem about 0.5cm in length with tweezers and scissors, such as figure 1 As shown, the tillering of the hybrids was significantly better than that of the parents, and the panicle length was between the parents, showing mid-parent dominance. The observation of tiller phenotype at seedling stage showed that the number of tillers in the hybrid was more than that of the parents, and the biomass was larger. The tiller joint samples were quickly frozen in liquid nitrogen and stored in a -80°C refrigerator. Each tissue was taken in 3 replicates to extract RNA. This period was regarded as the reproductive growth period of wheat, and RNA was extracted from each tissue three times to analyze the specific expression of TaHMT gene...

Embodiment 2

[0041] Example 2: Heat map analysis of tissue-specific expression of TaHMT gene family

[0042] To study the expression data of TaHMT gene family members in roots, stems, leaves and young ears of wheat at seedling stage, vegetative growth stage and reproductive growth stage. The results showed that the wheat TaHMT gene family was expressed in almost all of the above-mentioned periods and tissues, and there were large differences in the expression levels. Genes such as TaHMT64, TaHMT39, TaHMT76, TaHMT70, TaHMT72 were expressed at a high level in each stage of wheat growth and development. The expression levels of most TaHMT genes in roots, shoots, leaves and young panicles during vegetative growth and reproductive growth were significantly higher than those in other tissues, and they may be widely involved in various physiological activities of wheat growth and development. TaHMT4, TaHMT39, TaHMT69, TaHMT95 and other genes showed obvious tissue specificity, which was character...

Embodiment 3

[0043] Example 3 TaHMT Gene Expression Analysis in Hybrids

[0044] Based on the screening of wheat HMT family gene expression, the expression patterns of candidate genes in different hybrids were analyzed. TaHMT39 showed a high expression trend in the M4 male sterile line as the female parent combination; Presents a mid-affinity expression pattern and low expression in low-affinity material. At the same time, using Pearson correlation analysis, it was found that the TaHMT39 gene showed a positive correlation expression pattern in the M4 female parent combination, and its expression trend was consistent with the thousand-grain weight ( image 3 ), indicating that the histone methyltransferase participates in a certain regulatory network, and plays a very important regulatory function at the molecular level of yield heterosis in hybrids.

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Abstract

The invention relates to the field of gene engineering, in particular to a plant drought resistance related protein TaHMT39 and an encoding gene and application thereof. The amino acid sequence of theprotein is as shown in SEQ ID NO: 1, and the gene sequence of the protein is as shown in SEQ ID NO: 2. The yield heterosis related protein and the encoding gene thereof disclosed by the invention have very important theoretical and practical significance in improving and enhancing the wheat yield and accelerating the molecular breeding progress.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular, the invention relates to a two-line hybrid wheat yield heterosis-related protein TaHMT39 and its coding gene and application. Background technique [0002] The utilization of heterosis is of great significance for crop yield increase. Existing studies have shown that the genetic basis of heterosis is complex, including additive, dominance and epistatic effects, as well as complex interactions between them and the environment. Although it has been clarified that the genetic basis of heterosis comes from the heterozygosity of genotypes, a new regulatory system composed of paternal and maternal crosses regulates its hybrid F 1 Simply put, heterosis is the external manifestation of gene expression regulation under a new regulatory system. However, the expression of heterosis in hybrids is not controlled by the combination of all the genes of the parents. It is generally believed t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/10C12N15/54C12N15/84A01H5/00A01H6/46
CPCC12N15/8261C12N9/1007
Inventor 高世庆赵昌平孙仁玮公杰刘永杰李丹陈兆波王永波陈现朝
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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