Copper ion colorimetric detection method based on MnO2 complex enzyme stimulant

A detection method and technology for copper ions, which are applied in the field of colorimetric detection, can solve the problems of difficulty in achieving high-sensitivity detection of low-concentration target substances, difficulty in detection, and long time consumption, so as to avoid expensive equipment, improve analytical sensitivity, and easily obtain raw materials. Effect

Pending Publication Date: 2021-03-26
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] (1) Used to detect Cu 2+ Methods, such as absorption spectroscopy, electrochemical methods, and ion chromatography, these methods are costly, time-consuming, and require sophisticated equipment and professional operating technicians
[0010] (2) Currently, for Cu 2+ There are few studies on the simple analysis of detection, and the Cu 2+ It is difficult to detect in biological matrices, and it is difficult to achieve high-sensitivity detection of low-concentration targets

Method used

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  • Copper ion colorimetric detection method based on MnO2 complex enzyme stimulant
  • Copper ion colorimetric detection method based on MnO2 complex enzyme stimulant
  • Copper ion colorimetric detection method based on MnO2 complex enzyme stimulant

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Effect test

Embodiment 1

[0050] Such as Figure 1-7 As shown, a MnO-based 2 Copper ion colorimetric detection method of compound enzyme mimic, with copper ion Cu 2+ Taking detection as an example, the mechanism of action is:

[0051] MB / PANI / MnO 2 As a composite enzyme mimic, the composite material can catalyze the oxidation of TMB into blue cationic free radicals. L-Cys can effectively inhibit the generation of cationic free radicals and reduce them to colorless TMB molecules. L-Cys and Cu 2+ May form insoluble thiolates. In L-Cys with different concentrations of Cu 2+ After the reaction, the solution in MB / PANI / MnO 2 In the presence of composite materials and TMB, it turns blue, and the absorbance value at 652nm wavelength changes with the concentration of copper ions, so as to achieve the purpose of detecting copper ions. Using this feature, a simple, low-cost, Fast, efficient and stable new detection method.

[0052] The invention also discloses a MnO-based 2 The application of the copper ...

Embodiment 2

[0068] The preparation step of TMB solution comprises:

[0069] The TMB solution should be prepared and used immediately.

[0070]Liquid A: Dissolve 0.0143g TMB in 100μL DMSO, stir evenly, set aside;

[0071] Solution B: Add 24.3mL, 0.1mol / L citric acid to 5.7mL, 0.2mol / L NaH 2 PO 4 Stir evenly and set the volume to 100mL;

[0072] Then all the prepared A solution was added to 10mL B solution, and ultrasonicated to obtain a 6mmol / L TMB solution for the experiment, which was stored at 4°C in the dark.

Embodiment 3

[0074] Cu 2+ Colorimetric detection of:

[0075] S101: Weigh 0.0025g CuSO 4 ·5H 2 O made into 1mL, 10 7 nmol / L copper sulfate solution. Take 100μL, 10 7 nmol / L copper sulfate solution, diluted 10 times to get 10 6 nmol / L Cu 2+ ; Take 100μL, 10 6 nmol / L is diluted 10 times to get 10 5 nmol / L Cu 2+ ; Prepare 10 according to this method 4 nmol / L Cu 2+ 、10 3 nmol / L Cu 2+ 、10 2 nmol / L Cu 2+ .

[0076] Weigh 0.00048g of L-cys to prepare a 10mL, 400μmol / L solution, and store the prepared solutions at 4°C.

[0077] S102: the synthesis steps of MB include:

[0078] Take 30mL, 1.2mmol / L HCl, pass nitrogen gas for 20min, weigh 0.29815g FeCl 2 4H 2 O, 0.81087gFeCl 3 ·6H 2 O is placed in a container, add the above 30mL, 1.2mmol / L HCl solution with nitrogen gas, stir well until it is completely dissolved, and pass through the mixed solution with nitrogen gas for 20min, repeat the injection three times, then quickly add 30mL, 1.25mol / L NaOH, in nitrogen gas Stir vigorou...

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Abstract

The invention relates to a copper ion colorimetric detection method based on a MnO2 complex enzyme stimulant and application. The method comprises the following steps: preparing copper ions and L-cyssolutions with different concentrations; synthesizing an MB and MB/Au/PANI/MnO2 composite material; preparing a NaAC-HAC buffer solution; preparing a TMB solution; optimizing conditions; and performing colorimetric detection on the copper ions. According to the copper ion colorimetric detection method based on the MnO2 complex enzyme stimulant and the application, the MB/Au/PANI/MnO2 composite material serves as the complex enzyme stimulant and has high catalytic performance, high-sensitivity and quantitative detection of copper ions is achieved, the selectivity and stability of target objectdetection are improved, the compound enzyme stimulant is replaced with other MnO2-doped compounds which can be applied to detection and analysis of a new target object, the universality is high, the detection method is simple and convenient, the cost is low, the raw materials are easy to obtain and non-toxic, and the method can be applied to actual sample detection and is suitable for industrial popularization.

Description

technical field [0001] The invention belongs to the technical field of colorimetric detection, in particular to a MnO-based 2 Copper ion colorimetric detection method and application of complex enzyme mimics. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Copper is an essential trace element for the human body and plays an important role in a series of basic physiological processes and activities of the human body. Copper participates in the formation of various biological enzymes and maintains the activity of enzymes, so that electron transfer and redox metabolism life activities can proceed normally. Copper is also involved in red blood cell production to maintain normal hematopoietic function. In addition, children's convulsions are closely related to the copper content in the human body. However, excess copper ions (Cu 2+ ) can also lead to a range of health problems, including ischemic hear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/33
CPCG01N21/78G01N21/33G01N2021/775
Inventor 刘冰倩苏永欢蔡杰高荣
Owner GUIZHOU UNIV
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