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Klebsiella aerogenes and application thereof

A technology of Klebsiella and strains, applied in the field of Klebsiella aerogenes, can solve problems such as ecological environment pollution and human health threats

Active Publication Date: 2021-04-13
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cadmium is widely distributed in nature and its content is small. Its circulation is in a balanced state and will not cause pollution or harm to the environment; however, due to human int

Method used

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  • Klebsiella aerogenes and application thereof
  • Klebsiella aerogenes and application thereof
  • Klebsiella aerogenes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Screening, isolation, purification and identification of Example 1 bacterial strain S1

[0054] 1. Screening method for a strain S1 resistant to cadmium and removing cadmium ions

[0055] 1. Material preparation

[0056] Soil source for strain screening: Taken from the polluted soil of the paddy fields around Dabaoshan, Shaoguan City, Guangdong Province, the soil samples were sealed with sampling bags, and brought back to the laboratory at 4°C for storage for later use.

[0057] LB medium: peptone 10.0g, yeast extract powder 5.0g, NaCl 10.0g, pH 7.0-7.2, distilled water to 1L; solid medium plus 18.0g agar powder. Sterilize at 121°C for 15 minutes.

[0058] Inorganic salt medium (MSM medium): 5mL phosphate buffer solution (KH 2 PO 4 8.5g·L -1 、K 2 HPO 4 ·H 2 O2 1.75g·L -1 、Na 2 HPO 4 12H 2 O 33.4g·L -1 , NH 4 Cl 5.0g L -1 ), 3.0mL22.5g·L -1 MgSO 4 Solution (MgSO 4 ·7H 2 O 46.125g·L -1 ), 1.0mL 0.25g·L -1 FeCl 3 Solution (FeCl 3 ·6H 2 O 0.42g·L -...

Embodiment 2

[0081] Example 2 strain S1 containing Cd 2+ Cd in inorganic salt medium with peptone 2+ removal of

[0082] Streak the strain S1 on the LB plate, culture at 30°C for 20h, pick a single colony and inoculate it in the LB liquid medium, 30°C, 150r min -1 After culturing in the shaker for 20 hours, they were inoculated to Cd at a ratio of 2% (v / v) 2+ Concentrations of 10, 20, 40 and 80 mg·L -1 containing 10g·L -1 Tryptone was cultured in MSM medium, and samples were taken after 6 d (days); no addition of strain S1 was used as the control (CK), and three repeated experiments were set up. Sample liquid at 80000r·min -1 After centrifugation for 10 min, the supernatant was filtered with a 0.22 μm membrane filter, and its Cd was determined by flame atomic absorption spectrometry (AAS) after filtration. 2+ Residual amount (method is with embodiment 1).

[0083] Cd after 6 days of culture 2+ removal efficiency as image 3 Shown: The results show that after addition of strain S1,...

Embodiment 3

[0084] Phosphorus-dissolving and potassium-dissolving qualitative test of embodiment 3 bacterial strain S1

[0085] Solubilizing phosphorus and potassium of strain S1 (phosphate solubilizing bacteria, meaning converting insoluble phosphorus and potassium into soluble phosphorus and potassium) (specific references: Rawat, P., Das ,S.,Shankhdhar,D.,et al.,Phosphate-solubilizing microorganisms: Mechanism and their role in phosphate solubilization and uptake[J].Journal of Soil Science and Plant Nutrition.2020,https: / / doi.org / 10.1007 / s42729 -020-00342-7.) Functional qualitative test: Inoculate the strain S1 drawn on the LB solid medium into the LB liquid medium, shake and culture at 125~150rpm, 28~30°C for 18~24h, and then spot Connect on PKO solid medium and potassium bacteria solid medium, and inoculate 2% on PKO liquid medium and potassium bacteria liquid medium, establish 3 repeated experiments, place 30 ℃ incubator or shaker (125~150rpm ) 6d, observe the transparent circle fo...

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Abstract

The invention discloses Klebsiella aerogenes and application thereof. The strain is named Klebsiella aerogenes S1, the preservation number is GDMCC NO: 61041, and the strain is preserved in Guangdong Microbial Culture Collection Center on June 3<rd>, 2020, Floor 5, Building 59, #100 Yard, Middle Xianlie Road, Guangzhou. Experiments show that the Klebsiella aerogenes S1 can remove cadmium ions in soil and water in a co-metabolism mode and can also reduce the pH in a system so as to dissolve insoluble phosphorus and potassium substances, which indicates that the Klebsiella aerogenes S1 has the potential of removing the cadmium ions and improving the effective phosphorus and potassium content of the soil and can be used for environmental pollution control and plant growth promotion.

Description

technical field [0001] The invention belongs to the technical field of microbial restoration of inorganic pollutants, and in particular relates to a strain of Klebsiella aerogenes and its application. Background technique [0002] Cadmium (Cd), as one of the highly toxic heavy metal elements, is a non-essential element for the growth and development of the body, and has toxic effects on animals, plants and the human body. Cadmium is widely distributed in nature and its content is small. Its circulation is in a balanced state and will not cause pollution or harm to the environment; however, due to human intervention, the migration, loss and local enrichment of cadmium and its compounds are accelerated, resulting Serious ecological environment pollution poses a serious threat to human health. [0003] There are many ways for cadmium to enter the environment, among which surface runoff and industrial and mining wastewater discharge are the most important ways. Cadmium polluti...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34C09K17/14C05F11/08C05G3/00C05G3/80C12R1/22C02F101/20C09K101/00
CPCC12N1/20B09C1/10C02F3/34C09K17/14C05F11/08C05G3/00C05G3/80C02F2101/20C09K2101/00
Inventor 李永涛龚贝妮谭泽文徐会娟刘怡灵陈恋
Owner SOUTH CHINA AGRI UNIV
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