A kind of miRNA related to side chicken body weight and its application

Abstract

The invention discloses a miRNA related to the body weight of side chickens, and the nucleotide sequence of the miRNA is shown in SEQ ID NO.1. The invention also discloses specific primers for detecting the expression level of the miRNA related to the body weight of side chickens. The invention also discloses a detection kit, which includes the specific primer. The invention also discloses the application of the miRNA, the specific primer and the detection kit in judging the weight of the side chicken. The invention discloses a detection method of a detection kit. The present invention finally discloses a method for increasing or decreasing the body weight of side chickens. The detection kit provided by the invention can be used to detect the expression level of the miRNA in chicken muscle tissue, and the detection method is simple and quick. The present invention can also use the expression amount of the miRNA to control the body weight of the side chicken, and increase or decrease the weight of the side chicken according to actual needs in the actual breeding process.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a miRNA related to side chicken body weight and its application. Background technique [0002] Bian chicken is the only excellent original local chicken breed in Shanxi Province that has been included in the "National List of Genetic Resources Protection". With the improvement of people's demand for the quality of livestock products, local chicken breeds have received widespread attention due to their unique taste and flavor, and the demand has increased year by year. However, local chicken breeds generally grow slowly and feed utilization is low. Therefore, improving local chicken breeds The growth performance has become a research hotspot. [0003] miRNA is a kind of single-stranded endogenous non-coding RNA with a length of about 18-24 nt. In animals, it mainly acts by complementary binding between the seed sequence and the 3'UTR of mRNA, inhibiting mRNA translation ...

AI Technical Summary

Problems solved by technology

With the improvement of people's demand for the quality of livestock products, local chicken breeds have attracted widespread attention because of their unique taste and flavor, and the demand has increased year by year. However, local chicken breeds generally grow slowly and have low feed utilization. The growth performance of the

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