Preparation method of tumor tissue continuous pathological section

A technology of pathological slices and production methods, applied in the field of clinical medicine, can solve the problems of discontinuous slices and the inability to observe the growth characteristics of tumor tissues, achieve the effect of basically consistent surface thickness and flatness, and reduce bending deformation

Inactive Publication Date: 2021-04-13
THE PEOPLES HOSPITAL SHAANXI PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention overcomes the deficiencies of the prior art and provides a method for making continuous pathological slices of tumor tissue, aiming ...

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  • Preparation method of tumor tissue continuous pathological section
  • Preparation method of tumor tissue continuous pathological section
  • Preparation method of tumor tissue continuous pathological section

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Embodiment 1

[0048] Such as figure 2 and image 3 As shown, the method for making continuous pathological slices of tumor tissue provided in this embodiment, wherein the slices are sliced ​​in a spiral trajectory, includes the following steps:

[0049] S1. Pretreatment: select fresh tumor tissues of animals, infiltrate the fixative into the interior of the tissues by injection or perfusion, select a dehydrating agent to replace the water in the fixed tissues, and use a transparent agent for transparent treatment;

[0050] S2. Wax soaking and embedding: paraffin wax soaks the tissue to replace the transparent agent; the wax soaked tissue is placed in melted solid paraffin, and after the paraffin solidifies, the tissue is embedded to form a wax block 1;

[0051] S3. Slicing: After trimming the wax block 1, the brazing tube 2 passes through the central axis of the wax block 1, and both ends of the brazing tube 2 are connected to the rotary table 3, and the wax block 1 is fixed up and down; ...

Embodiment 2

[0064] Such as Figure 4 and Figure 5 As shown, this embodiment is based on the method for making continuous pathological slices of tumor tissue provided in Embodiment 1, and improvements have been made in slicing and installation, and slicing in concentric circles includes the following steps:

[0065] a1. Pretreatment: select fresh tumor tissues of animals, infiltrate the fixative into the interior of the tissues by injection or perfusion, select a dehydrating agent to replace the water in the fixed tissues, and use a transparent agent for transparent treatment;

[0066] a2. Wax dipping and embedding: the paraffin wax is soaked into the tissue to replace the transparent agent; the wax soaked tissue is placed in melted solid paraffin, and after the paraffin solidifies, the tissue is embedded to form a wax block 1;

[0067] a3. Slicing: Place the wax block 1 in the matching cutting groove 5 and fix it through the metal hoop 6. The annular slicing knife 4 is placed directly a...

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Abstract

The invention discloses a preparation method of a tumor tissue continuous pathological section, which comprises the steps of S1, selecting fresh tumor tissue, and carrying out fixation, dehydration and transparent treatment; S2, carrying out wax dipping and embedding treatment on the tissue; S3, fixing the wax block, and slicing in a concentric circle mode or slicing in a spiral track mode along any point of the outer edge surface of the wax block; S4, spreading, specifically, dividing the slice in the step S3 into different samples, numbering the samples, spreading the samples on the water surface of 40-45 DEG C, and naturally flattening the slightly wrinkled samples; S5, pasting and baking the slices; and S6, dyeing and sealing. According to the slicing method, a traditional parallel slicing method according to a certain interval distance is abandoned, a continuous slicing method in a concentric circle mode or a spiral track mode is adopted, clinically, the slicing mode can effectively help doctors to observe the growth characteristics of tumor tissue from inside to outside and observe the three-dimensional whole tissue state, so that the difficulty of clinically judging pathology is reduced and the accuracy of clinically judging pathology is improved.

Description

technical field [0001] The invention relates to the technical field of clinical medicine, in particular to a method for making continuous pathological slices of tumor tissue. Background technique [0002] Clinically, doctors need to conduct pathological biopsy to judge whether an organ is a tumor lesion. The nature of the tumor, whether it is benign or malignant, can only be ascertained through the pathological examination of the tumor. Benign tumors often do not recur after resection and do not require follow-up treatment. After the malignant tumor is resected, it often needs follow-up treatment, such as radiotherapy, chemotherapy, etc., so pathological examination is very important in clinical examination. [0003] Traditional pathological sections of tumor tissue are generally sliced ​​in parallel at a certain interval. This section method cannot effectively observe the growth characteristics of the tumor tissue from the inside to the outside, and there is no continuity...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30G01N1/36
CPCG01N1/286G01N1/30G01N1/36G01N2001/2873
Inventor 岳青芳王飞曹菲张媛
Owner THE PEOPLES HOSPITAL SHAANXI PROV
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