In-vitro preservation method of hemp seed potato test-tube plantlets

A technology for in vitro preservation and test tube seedlings is applied in the field of agricultural production, which can solve the problems of high and low temperature, strong ultraviolet rays, insufficient nutrition, harsh living environment, and low survival rate of transplanting, so as to increase the stress resistance of plants and improve the survival rate of plants. rate, slowing down plant growth

Active Publication Date: 2021-04-16
SHANGRAO NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Huaiyu mountain hempseed artichoke has been located in high-altitude alpine areas for a long time, and its living environment is much worse than other plain areas. It has been subjected to factors such as high and low temperature, strong ultraviolet rays, and insufficient nutrition all year round. In order to adapt to this harsh environment, the plants themselves will do and adapt to its environmental changes, and then formed a unique potato variety adapted to the natural environment of Huaiyu Mountain. Once this potato leaves this special environment and is transplanted to other areas, it will break the original adversity adaptation. Mechanism, variation occurs, resulting in low survival rate of transplanting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for in vitro preservation of hempseed artichoke test-tube plantlets, comprising the following steps:

[0029] Step 1: Place Huaiyu Mountain hempseed artichoke seedlings detoxified by ultra-low temperature therapy (stored in the Life Science Experimental Teaching Center of Shangrao Normal University) in 30°C and 45°C water baths for 30 minutes to complete thawing; after thawing, transfer to MS medium At 25°C, the light intensity is 2500lux, and the light is illuminated for 20h to complete the activation.

[0030] Step 2: Transfer the detoxified seedlings activated in step 1 to the improved MS medium for recovery, heat treatment at 80°C for 1 minute from the 6th hour of recovery, and then continue to culture at 23°C for 2 hours, then heat treatment at 80°C for 1 minute, and repeat for 5 times, to obtain resuscitated test-tube plantlets;

[0031] The improved MS medium contains 30g / L sucrose, 25g / L mannitol, 0.05μg / L abscisic acid, 0.05μg / L paclobutrazol and 20g / ...

Embodiment 2

[0037] A method for in vitro preservation of hempseed artichoke test-tube plantlets, comprising the following steps:

[0038] Step 1: Place Huaiyu Mountain alpine hempseed artichoke seedlings detoxified by ultra-low temperature therapy in water baths at 30°C and 45°C for 35 minutes to complete thawing; after thawing, transfer to MS medium at 24°C, light intensity 2800lux, and light for 22 hours to complete activation .

[0039] Step 2: Transfer the virus-free seedlings activated in step 1 to the improved MS medium for recovery, heat treatment at 80°C for 2 minutes from the 6th hour of recovery, and then continue to culture at 23°C for 3 hours, then heat treatment at 80°C for 2 minutes, and repeat for 6 hours. times, to obtain resuscitated test-tube plantlets;

[0040] The improved MS medium contains 33g / L sucrose, 27g / L mannitol, 0.08μg / L abscisic acid, 0.08μg / L paclobutrazol and 25g / L activated carbon;

[0041]Step 3: Transplant the resuscitated test-tube plantlets of step ...

Embodiment 3

[0046] A method for in vitro preservation of hempseed artichoke test-tube plantlets, comprising the following steps:

[0047] Step 1: Place Huaiyu Mountain alpine hempseed artichoke seedlings detoxified by ultra-low temperature therapy in water baths at 30°C and 45°C for 40 minutes to complete thawing; after thawing, transfer to MS medium at 25°C, light intensity 3000lux, and light for 24 hours to complete activation .

[0048] Step 2: Transfer the virus-free seedlings activated in step 1 to the improved MS medium for recovery, heat treatment at 80°C for 3 minutes from the 8th hour of recovery, and then continue to culture at 23°C for 3 hours, then heat treatment at 80°C for 3 minutes, and repeat for 8 hours. times, to obtain resuscitated test-tube plantlets;

[0049] The improved MS medium contains 35g / L sucrose, 30g / L mannitol, 1μg / L abscisic acid, 1μg / L paclobutrazol and 30g / L activated carbon;

[0050] Step 3: Transplant the resuscitated test-tube plantlets of step 2 int...

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Abstract

The invention discloses an in-vitro preservation method of hemp seed taro test-tube plantlets. The in-vitro preservation method comprises the following steps: carrying out rapid thawing treatment on ultralow-temperature therapy virus-free seedlings of alpine hemp seed taro in Waihu Mountain for unfreezing, and then transferring the seedlings to an MS culture medium for activation; transferring the activated virus-free seedlings into an improved MS culture medium for resuscitation, and carrying out auxiliary treatment by adopting a cold-hot interval treatment mode in the resuscitation process to obtain resuscitated test-tube seedlings; transplanting the resuscitated test-tube plantlets into a greenhouse with a substrate, and carrying out root irrigation treatment by using a proliferation and seedling strengthening culture medium to obtain hemp seed taro seedlings; and transplanting the obtained hemp seed taro seedlings to a field for planting after being subjected to conventional seedling hardening treatment. According to the in-vitro preservation method, the virus-free seedlings are subjected to cold-hot interval treatment and domestication, the components of the culture medium are optimized, so that the in-vitro storage life of the test-tube seedlings of the Muscodendron mucronatum is prolonged, and the survival rate of the transplanted test-tube seedlings is increased.

Description

technical field [0001] The invention relates to the technical field of agricultural production, in particular to a method for in vitro preservation of hemp seed artichoke test-tube plantlets. Background technique [0002] Hempseed yam, scientific name potato, belongs to Solanaceae, an annual herb plant with edible tubers, and is the fourth most important food crop in the world, second only to wheat, rice and corn. Huaiyu Mountain belongs to the mid-subtropical humid monsoon region, with an annual average temperature of 12-16°C. The climate in the territory is cool, with moderate rainfall and sufficient sunlight, making it suitable for growing potatoes. Huaiyushan hemp seed yam, also known as Huaiyushan potato, has oval or oblong tubers and extremely rough potato skins, which are obviously different from ordinary potatoes. The color is yellow and the potato flesh is white. Moreover, Huaiyushan potatoes are rich in nutrition and do not contain reducing sugar, so they are suit...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A01H4/00
Inventor洪森荣曾晓健万双燕张清招朱珊珊尹明华
OwnerSHANGRAO NORMAL UNIV