Breeding method for inducing corn apomixis by using mixed reagent and application

An apomixis, corn technology, applied in the fields of application, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of difficult selection of haploid grains, slow operation speed, carcinogenicity of reagents, etc., to improve induction effect, treatment The effect of high speed and reduced breeding costs

Active Publication Date: 2021-05-11
INNER MONGOLIA UNIV FOR THE NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]However, there are problems such as difficult selection and doubling of haploid seeds in the induction method, and the use of chemical induct

Method used

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  • Breeding method for inducing corn apomixis by using mixed reagent and application
  • Breeding method for inducing corn apomixis by using mixed reagent and application
  • Breeding method for inducing corn apomixis by using mixed reagent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]This example provides a breeding method that uses a mixed reagent to induce corn non-fusion reproduction, including the following steps:

[0040](1) Inducer selection:

[0041]Compound A includes fluorol-Leling and colostrum in volume ratio of 1: 1;

[0042]The compound B includes a volume ratio of 1: 1 fluoromide;

[0043]Compound C includes a volume ratio of 1: 1: 1: 1: 1: Chitin and pentarmine;

[0044]The compound D includes a volume ratio of 1: 1 of nullin and pentynoxythylamine;

[0045]Two sets of controls were set, and the control A included fluoflings, and the control B included autumn water;

[0046](2) Partition: The field is divided into the number of inducers, divided into compound A, compound B, compound C area, compound D area, control A, control B;

[0047](3) Operation processing: All districts have stringent bags in front of the corn, 3 days before the spings, and use the sharp pliers to cut the silk at the top of the jab. When the filament of the bare is cut, slow Slowly pull out t...

Embodiment 2

[0053]This example provides a breeding method that uses a mixed reagent to induce corn non-fusion reproduction, including the following steps:

[0054](1) Inducer selection:

[0055]Compound A includes a volume ratio of 1: 1.2 of fluorol-Leling and columbium;

[0056]The compound B includes a volume ratio of 1: 1.2 fluoromide;

[0057]Compound C includes a volume ratio of 1: 1.2: 1.2 of fluorol, green and pentythylamine;

[0058]Compound D includes a volume ratio of 1: 1.2 of penthuslamine;

[0059]Two sets of controls were set, and the control A included fluoflings, and the control B included autumn water;

[0060](2) Partition: The field is divided into the number of inducers, divided into compound A, compound B, compound C area, compound D area, control A, control B;

[0061](3) Operation processing: All districts have strict sleeves in front of the corn feeding in front of the zone. After 5 days, the tip is cut with the mouth of the mouth, when the filament of the bide is bullied, slow Slowly pull out ...

Embodiment 3

[0066]This example provides a breeding method that uses a mixed reagent to induce corn non-fusion reproduction, including the following steps:

[0067](1) Inducer selection:

[0068]Compound A includes a volume ratio of 1: 1.1 Fluond and green;

[0069]The compound B includes a volume ratio of 1: 1.1 fluoromamma;

[0070]Compound C includes a volume ratio of 1: 1.1: 1.1 Fluond, green and pentarne;

[0071]The compound D includes a volume ratio of 1: 1.1 of the penthuslamine;

[0072]Two sets of controls were set, and the control A included fluoflings, and the control B included autumn water;

[0073](2) Partition: The field is divided into the number of inducers, divided into compound A, compound B, compound C area, compound D area, control A, control B;

[0074](3) Operation processing: All districts have stringent bags in the tabs in front of the zone, 4 days after the sputum, with a sharp plunge in the top of the jab, when it feels that the floral wire is cut off, slow Slowly pull out the filament to fo...

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Abstract

The invention is suitable for the technical field of corn breeding, and provides a breeding method for inducing corn apomixis by using a mixed reagent and application. The breeding method for inducing the corn apomixis by using the mixed reagent comprises the following steps of selecting inducers; performing partitioning; performing inducer treatment, wherein the inducer treatment comprises the following steps of strictly bagging corn ears before silking in each area, clamping and cutting silks at the top ends of bracts by using a pair of nipper pliers after silking, slowly pulling out the silks to form cavities when the silks in the bracts are cut off, injecting the inducers corresponding to all areas, and then sleeving with paper bags again; screening the inducers; carrying out optimal inducer treatment; and harvesting. According to the breeding method, by improving the inducers, the safety problem, the pollen pollution problem and the operation efficiency problem in reagent use are solved, the induction effect is improved while safety is guaranteed, the breeding speed of corn inbred lines can be increased, the inductivity is high, and the genetic stability of germplasm resources can be guaranteed.

Description

technical field [0001] The invention belongs to the technical field of corn breeding, and in particular relates to a breeding method for inducing apomixis of corn by using a mixed reagent and its application. Background technique [0002] In the conventional method of breeding maize inbred lines, at least 7 generations of continuous selfing are required to complete the selection of an inbred line, and even more than 10 generations for materials with complex genetic backgrounds. In order to speed up the breeding speed, the traditional method is to use the induction line to induce haploids and then double or use a single chemical agent such as colchicine to induce. [0003] However, there are problems such as difficult selection and doubling of haploid grains in the induction method, while chemical induction methods such as colchicine have problems such as reagent carcinogenicity, low induction rate, pollen pollution, slow operation speed and low efficiency. Contents of the ...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01H6/46
CPCA01H1/06A01H6/46
Inventor 王晓东李志刚刘鹏向殿军曹霞张巍巍姚威港史铎
Owner INNER MONGOLIA UNIV FOR THE NATITIES
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