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Nano-drug for tumor photo-thermal and iron agent combined treatment and preparation method of thereof

A drug and therapeutic technology, applied in the field of metal-protein nano-medicine and its preparation, can solve the problems of application limitation, uncontrollable shape and size, poor biocompatibility, etc., and achieve good killing effect

Active Publication Date: 2021-05-14
GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this complex has the characteristics of uncontrollable shape an

Method used

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  • Nano-drug for tumor photo-thermal and iron agent combined treatment and preparation method of thereof
  • Nano-drug for tumor photo-thermal and iron agent combined treatment and preparation method of thereof
  • Nano-drug for tumor photo-thermal and iron agent combined treatment and preparation method of thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0039]Example 1

[0040]Metal-protein nano drug particles Fe-Ga @ BSA-SRF, its preparation method includes the following steps:

[0041](1) Weigh 66 mg of BSA in ultra-pure water and stirred at room temperature for 30 minutes;

[0042](2) 54 μL of FeCl3Solution (100 mg / ml) was added dropwise to the BSA solution under conditions of stirring at room temperature, and stirred at room temperature for 30 min;

[0043](3) 1.02 ml of the Nothic Acid (10 mg / mL) was added dropwise to the Fe-BSA mixed solution under stirring, and then the protopeer was allowed to stand for 3 hours;

[0044](4) The resulting nanoparticle solution was filtered with ultrafiltration tubes, and 2 times in ultrapure water was washed, and the purified Fe-Ga @ BSA nanoparticles were obtained; the atomic force microscope results were obtained.figure 1 The nanoparticle size is 1, and the resulting nanopartic external light heat-warmest curve is shown in the amount of the resulting nanoparticle.figure 2 It can be seen from the figu...

Example Embodiment

[0047]Example 2

[0048]A metal-protein nano drug particles are Fe-Ga @ BSA-SRF protein-metal nano-drug particles prepared by the method of the present invention, including the following steps:

[0049]Step (1) in Example 1

[0050](2) 27 μL of FeCl3Solution (100 mg / ml) was added dropwise to the BSA solution under conditions of stirring at room temperature, and stirred at room temperature for 30 min;

[0051]Step (2) - (6) FE-Ga @ BSA-SRF nano drug particles having a lower unit Fe content of the unit Fe content.

Example Embodiment

[0052]Example 3

[0053]A metal-protein nano drug particles are Fe-Ta @ OVA-SRF protein-metal nano-drug particles prepared by the method of the present invention, including the following steps:

[0054](1) Weigh 45 mg OVA in ultra-pure water and stirred at room temperature for 30 minutes;

[0055](2) 54 μL of FeCl3Solution (100 mg / ml) was added dropwise to the OVA solution at room temperature and stirred at room temperature for 30 min;

[0056](3) 2.04 ml of tannic acid (TA) solution (25 mg / ml) was added dropwise to the Fe-OVA mixed solution under stirring, followed by depressing the lateral temperature for 24 hours;

[0057](4) The resulting nanoparticle solution was filtered with a supercapsal tube and washed 2 times with ultrapure water, and the purified Fe-Ta @ OVA nanoparticles were obtained;

[0058](5) FE-TA @ OVA prepared by 1 ml of 5 mm (4) was added to 1 ml PBS, and 50 μl of Solafini solution (40 mg / mL) was added under vigorous stirring conditions, and the mixture was used to use wate...

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Abstract

The invention discloses a nano-drug for tumor photo-thermal combined iron agent treatment and a preparation method thereof, Fe < 3 + > ions are compounded on carrier protein of metal-protein nano-particles, and meanwhile, pharmaceutically acceptable polyphenol compounds are chelated through the Fe < 3 + > ions. Through experiments, the inventor discovers that different from a traditional method that a Fenton reagent generated in a tumor photothermal treatment process can cause oxidative degradation failure of drug molecules or uncontrollable drug effects, a specific ferroptosis drug is compounded with metal-protein nanoparticles containing polyphenol compounds and used for tumor photothermal treatment; the specific ferroptosis drug and themetal-protein nanoparticles unexpectedly have a synergistic effect and have a better killing effect on tumor cells.

Description

technical field [0001] The invention relates to a metal-protein nano-medicine carrier, in particular to a metal-protein nano-medicine for tumor photothermal therapy combined with ferroptosis, a preparation method and application thereof. Background technique [0002] Cancer is a major disease that threatens human health worldwide. Currently, the main methods for treating cancer include chemotherapy, radiotherapy, and surgical resection. However, these methods are often accompanied by high toxicity, recurrence rate, and low targeting. In recent years, a variety of new biomedical nanomaterials have been used in cancer treatment, including photothermal and photodynamic therapy materials, nano drug carrier materials, and the use of tumor cell microenvironment to induce Fenton reaction (Fenton reaction) to induce tumors. Nanomaterials for cellular ferroptosis. Photothermal therapy combined with ferroptosis for tumor therapy is a new and effective method that can solve the shortc...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K45/06A61K47/64A61P35/00B82Y5/00B82Y20/00B82Y30/00B82Y40/00
CPCA61K41/0052A61K45/06A61K47/643A61P35/00B82Y5/00B82Y30/00B82Y40/00B82Y20/00
Inventor 杨斌余馨颖韩世松谢国喜李雨微蔡燕君尚同祎郑国栋
Owner GUANGZHOU MEDICAL UNIV
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