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Recombinant cd1-restricted t cells and methods

A restricted, cell-based technology for cell-based immunotherapy that addresses issues such as being unsuitable for clinical settings, complex, and difficult to obtain vaccines

Pending Publication Date: 2021-05-14
南特生物公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these are conceptually simple and attractive approaches, effective vaccines remain elusive
In yet another known method, CD1b-restricted T cells from various donors can be expanded to relatively large numbers (see, e.g., US 2003 / 0153073), but such expansion usually requires complex process, and are often not appropriate for clinical settings

Method used

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  • Recombinant cd1-restricted t cells and methods
  • Recombinant cd1-restricted t cells and methods
  • Recombinant cd1-restricted t cells and methods

Examples

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[0047] The following description provides one of ordinary skill in the art with exemplary guidance for the preparation and use of the recombinant cells described herein. However, these examples should not be construed as limiting the inventive subject matter.

[0048] To study the effect of T cell stimulation and type of transfection on T cell responses to various antigens, 'naked' non-targeting PBAE (poly(β-amino ester)) nanoparticles associated with the following two types of RNA were transfected separately. T cells were stained: RNA encoding clone 18 TCR (bicistronic construct with clone 18 TCR alpha and beta chains) and RNA encoding GFP (green fluorescent protein). In this case, isolated T cells were cultured for 24 hours with a mixture of anti-CD3 and anti-CD28 antibodies (Immunocult Human CD3 / CD28 T Cell Activator, Stemcell Technologies) and then before transfection Stimulation was achieved by culturing for an additional 24 hours in unsupplemented medium. PBAE-RNA nano...

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Abstract

T cells are transfected with a recombinant RNA molecule that encodes at least one of an alpha chain and a beta chain of a CD1b restricted T cell receptor. Preferably, the so prepared T cells are used as a cell-based therapeutic composition to treat tuberculosis.

Description

[0001] This application claims priority to our co-pending U.S. Provisional Patent Application Serial No. 62 / 716,292 filed August 8, 2018, which is hereby incorporated by reference. [0002] sequence listing [0003] The ASCII text file of the 8KB Sequence Listing, titled 102719.0013PCT_ST25, was created on July 19, 2019 and filed electronically with this application via EFS-Web, the contents of which are incorporated by reference in their entirety. technical field [0004] The field of the invention is cell-based immunotherapy, in particular involving the use of genetically modified T cells expressing at least a portion of the CD1b-restricted T cell receptor. Background technique [0005] The Background Description includes information useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, nor is any publication specifically or implicitly referenced to be...

Claims

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Application Information

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IPC IPC(8): C12N5/0783C07K14/725C12N9/24C12N15/87A61K35/17A61P31/06
CPCC12N15/87A61P31/06C07K14/70596C12N9/2402C12Y302/01132A61K39/464817A61K39/4611C12N5/0636A61K35/17C07K14/7051C12N15/625C12N15/85C12N2800/107
Inventor W·理查森T·吴P·西林
Owner 南特生物公司
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