Anti-cadmium pollution mandarin fish muscle repairing agent and application thereof
A remediation agent, cadmium pollution technology, applied in the field of agricultural products and molecular biology, can solve problems such as pollution
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Embodiment 1
[0029] Embodiment 1: anti-cadmium pollution mandarin fish muscle repair agent is to Cd 2+ Effects of Exposure on Growth Indicators of Mandarin Fish
[0030] 1. Anti-cadmium pollution mandarin fish muscle repair agent, including edible agent and injection, wherein the edible agent is calculated by mass percentage and mixed with the following components:
[0031] Taurine 20-35%;
[0032] Arachidonic acid 20-35%;
[0033] Sodium butyrate 30-45%;
[0034] Vitamin D3 5-10mg / kg; (0.0005-0.001%)
[0035] Pex10 protein 400 mg / kg (0.04%).
[0036] Preferably, the edible preparation is mixed by the following components in terms of mass percentage:
[0037] Taurine 30%;
[0038] Arachidonic acid 30%;
[0039] Sodium butyrate 39.959%;
[0040] Vitamin D3 0.001%;
[0041] Pex10 Protein 0.04%.
[0042] The Pex10 protein of mandarin fish can be obtained by the following ways: collect late stage embryos of mandarin fish and break them, centrifuge the broken embryos at 1200-1500r / min f...
Embodiment 2
[0048] Example 2 The effect of anti-cadmium pollution mandarin fish muscle repair agent on the expression of pex10 gene in mandarin fish muscle
[0049] There are five groups in this embodiment, the first group is the control group fed with common diet; the second group is Cd 2+ + normal ration feeding group; the third group is Cd 2+ +1% food additive group; the fourth group mandarin fish Cd 2+ +Injection group, injected once every 15 days (0.1μg / g); the fifth group was Cd 2+ + Feed added edible agent + injection group. The breeding time is 2 months, and the initial weight of mandarin fish is 22-27g. Cd 2+ Indicates cadmium exposure, Cd 2+ The exposure concentration was 10 μg / L, and the statistical results of pex10 gene expression in mandarin fish muscle in each group were as follows: figure 1 shown.
Embodiment 3
[0051] Effects of anti-cadmium pollution mandarin fish muscle repair agent on the expression of myosin light chain protein gene (mlc3) and chronic myosin heavy chain protein l gene (smyhc1) in mandarin fish muscle
[0052] The statistical results of myosin light chain protein gene (mlc3) and chronic myosin heavy chain protein 1 gene (smyhc1) expression in mandarin fish muscle are as follows figure 2 with image 3 , a total of 5 groups were established, the first group was the control group fed with common diet; the second group Cd 2+ + normal ration feeding group; the third group is Cd 2+ +1% added food additives; the fourth group of mandarin fish Cd 2+ + injection, once every 15 days (0.1μg / g); the fifth group is Cd 2+ + Feed additives + injections. The breeding time is 2 months, and the initial weight of mandarin fish is 22-27g. The statistical results of the expression of mlc3 gene in mandarin fish muscle in each group are as follows: figure 2 As shown, the statisti...
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