Anti-cadmium pollution mandarin fish muscle repairing agent and application thereof

A remediation agent, cadmium pollution technology, applied in the field of agricultural products and molecular biology, can solve problems such as pollution

Active Publication Date: 2021-05-18
CHANGSHA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A considerable amount of cadmium is discharged into the environme...

Method used

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  • Anti-cadmium pollution mandarin fish muscle repairing agent and application thereof
  • Anti-cadmium pollution mandarin fish muscle repairing agent and application thereof
  • Anti-cadmium pollution mandarin fish muscle repairing agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: anti-cadmium pollution mandarin fish muscle repair agent is to Cd 2+ Effects of Exposure on Growth Indicators of Mandarin Fish

[0030] 1. Anti-cadmium pollution mandarin fish muscle repair agent, including edible agent and injection, wherein the edible agent is calculated by mass percentage and mixed with the following components:

[0031] Taurine 20-35%;

[0032] Arachidonic acid 20-35%;

[0033] Sodium butyrate 30-45%;

[0034] Vitamin D3 5-10mg / kg; (0.0005-0.001%)

[0035] Pex10 protein 400 mg / kg (0.04%).

[0036] Preferably, the edible preparation is mixed by the following components in terms of mass percentage:

[0037] Taurine 30%;

[0038] Arachidonic acid 30%;

[0039] Sodium butyrate 39.959%;

[0040] Vitamin D3 0.001%;

[0041] Pex10 Protein 0.04%.

[0042] The Pex10 protein of mandarin fish can be obtained by the following ways: collect late stage embryos of mandarin fish and break them, centrifuge the broken embryos at 1200-1500r / min f...

Embodiment 2

[0048] Example 2 The effect of anti-cadmium pollution mandarin fish muscle repair agent on the expression of pex10 gene in mandarin fish muscle

[0049] There are five groups in this embodiment, the first group is the control group fed with common diet; the second group is Cd 2+ + normal ration feeding group; the third group is Cd 2+ +1% food additive group; the fourth group mandarin fish Cd 2+ +Injection group, injected once every 15 days (0.1μg / g); the fifth group was Cd 2+ + Feed added edible agent + injection group. The breeding time is 2 months, and the initial weight of mandarin fish is 22-27g. Cd 2+ Indicates cadmium exposure, Cd 2+ The exposure concentration was 10 μg / L, and the statistical results of pex10 gene expression in mandarin fish muscle in each group were as follows: figure 1 shown.

Embodiment 3

[0051] Effects of anti-cadmium pollution mandarin fish muscle repair agent on the expression of myosin light chain protein gene (mlc3) and chronic myosin heavy chain protein l gene (smyhc1) in mandarin fish muscle

[0052] The statistical results of myosin light chain protein gene (mlc3) and chronic myosin heavy chain protein 1 gene (smyhc1) expression in mandarin fish muscle are as follows figure 2 with image 3 , a total of 5 groups were established, the first group was the control group fed with common diet; the second group Cd 2+ + normal ration feeding group; the third group is Cd 2+ +1% added food additives; the fourth group of mandarin fish Cd 2+ + injection, once every 15 days (0.1μg / g); the fifth group is Cd 2+ + Feed additives + injections. The breeding time is 2 months, and the initial weight of mandarin fish is 22-27g. The statistical results of the expression of mlc3 gene in mandarin fish muscle in each group are as follows: figure 2 As shown, the statisti...

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PUM

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Abstract

The invention relates to an anti-cadmium pollution mandarin fish muscle repairing agent. The repairing agent comprises an edible agent and an injection, wherein the edible agent consists of taurine, arachidonic acid, sodium butyrate, vitamin D3 and Pex10 protein; and the injection is a single-stranded small RNA solution. Single-stranded small RNA is modified by a sulfo-backbone, a 3' terminal cholesterol and a complete sequence base methoxyl. The edible agent synergistically assists the small RNA molecules to play a role, and the small RNA molecules and the edible agent stimulate activation of peroxisome to reduce muscle lipid peroxidation and protein oxidative damage, so that muscle tissues can repair oxidative damage caused by cadmium pollution in time. The repairing agent is favorable for recovering the muscle texture of the mandarin fish and has a wide market prospect.

Description

technical field [0001] The invention belongs to the technical field of agricultural products and molecular biology, and relates to a cadmium-polluted mandarin fish muscle repair agent and an application thereof. Background technique [0002] Cadmium (Cd) is ubiquitous in nature. Cadmium and zinc are congener elements. In nature, cadmium often coexists with zinc and lead. When the environment is polluted by cadmium, cadmium can be enriched in organisms and enter the human body through the food chain to cause chronic poisoning. Especially after the water environment is polluted, it has brought a serious threat to the healthy breeding of fish and the meat quality of aquatic products in our country. The toxicity of cadmium is closely related to oxidative stress in the body, which can induce the generation of lipid peroxide in cells, thereby inducing oxidative damage. [0003] Since the discovery of cadmium in the early 20th century, the output of cadmium has increased year by ...

Claims

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Application Information

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IPC IPC(8): A23K20/105A23K20/142A23K20/147A23K20/153A23K20/158A23K20/174A23K50/80
CPCA23K20/142A23K20/158A23K20/105A23K20/174A23K20/147A23K20/153A23K50/80
Inventor 包凌晟褚武英黄鑫朱鑫张建社潘亚雄
Owner CHANGSHA UNIVERSITY
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