Method for rapidly enriching glucose utilization type caproic acid producing flora from pit mud and application

A type of caproic acid bacteria and glucose technology, applied in the field of rapid enrichment of glucose-utilizing caproic acid-producing bacteria from pit mud, can solve the problem of low abundance of sugar-utilizing caproic acid bacteria, poor ability to produce caproic acid, side effects Problems such as high content of butyric acid in the product

Active Publication Date: 2021-05-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The present invention aims to solve the technical problems of low abundance of sugar-utilizing caproic acid bacteria, poor abili...

Method used

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  • Method for rapidly enriching glucose utilization type caproic acid producing flora from pit mud and application
  • Method for rapidly enriching glucose utilization type caproic acid producing flora from pit mud and application
  • Method for rapidly enriching glucose utilization type caproic acid producing flora from pit mud and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1: the screening of qualified pit mud

[0063] Specific steps are as follows:

[0064] 1. Collection of pit mud samples

[0065] Multiple groups of fresh pit mud were collected from 5 pits in normal continuous use, each group was named PM1, PM2, PM3, PM4, PM5, and 5 to 10 pit bottom pit mud samples were sampled in each group according to the five-point sampling method , the sampling depth is 1-3cm, the collected pit mud samples are put into a sealed bag together with the gas-producing bag and sealed for use, the gas-producing bag absorbs the oxygen in the sealed bag and releases carbon dioxide, creating a good environment for the collected pit mud samples. An anaerobic environment, to avoid the pit mud sample being exposed to oxygen for too long will lead to the inactivation of anaerobic bacteria in the pit mud.

[0066] 2. Preparation of enrichment medium for anaerobic caproic acid-producing bacteria

[0067] Use an analytical balance to weigh 15.0 g of g...

Embodiment 2

[0078] Example 2: Obtaining of sugar-utilizing anaerobic caproic acid-producing bacteria

[0079] In this example, the qualified pit mud PM2 obtained through screening in Example 1 was selected.

[0080] Specific steps are as follows:

[0081] 1. The fermented liquid inoculated with PM2 pit mud obtained in the step 4 of embodiment 1 is used as the first generation enrichment culture liquid F1;

[0082] Transfer the first-generation enrichment medium F1 into the fresh enrichment medium prepared in step 2 of Example 1 at an inoculum size of 5%-10% (v / v), and obtain after culturing at 37°C for 4 days The second-generation enrichment medium F2;

[0083] Transfer the second-generation enrichment medium F2 into the fresh enrichment medium prepared in step 2 of Example 1 at an inoculum size of 5%-10% (v / v), and obtain after culturing at 37°C for 4 days The third-generation enrichment medium F3;

[0084] Transfer the third-generation enrichment medium F3 into the fresh enrichment ...

Embodiment 3

[0094] Example 3: Application of Sugar Utilizing Caproic Acid Bacteria

[0095] In this embodiment, the fermented liquid of anaerobic caproic acid-producing bacteria group involved in the present invention is used to carry out cellar fermentation strengthening operation, including the following steps:

[0096] (1) Preparation of seed solution 2: according to the PM2 obtained in step (1) in the embodiment 2, the F4 generation enrichment culture solution 100mL of the pit mud is used as the seed solution 1, and the seed solution 1 (OD600=2.77 ) with 10% (v / v) inoculum size inoculated in the enrichment medium (the volume of the fermenter is 3L, other components and their preparation method are the same as the preparation method in the step 2 of Example 1), and the flow rate is 2L / min high-purity nitrogen to maintain the anaerobic environment in the fermenter, and the fermentation temperature was controlled at 37°C to obtain seed solution 2.

[0097] (2) Second stage amplificatio...

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Abstract

The invention discloses a method for rapidly enriching glucose utilization type caproic acid producing flora from pit mud and application, and belongs to the technical field of brewing microorganisms and biology. By adopting the method for improving the abundance and caproic acid producing capacity of the glucose utilization type caproic acid producing flora in the pit mud, the glucose utilization type caproic acid producing flora and a bacterial solution thereof are obtained, the caproic acid yield is in a continuous rising trend, and through continuous passage, the caproic acid yield is increased to 9.5g/L from 1.3g/L; in the enriching process, the relative abundance of caproic acid bacteria microorganisms capable of utilizing glucose is gradually increased and increased to 43% from 12%. The method can be used for improving the acidity and ester flavor of baijiu and can also be applied to caproic acid fermentation with glucose as a carbon source.

Description

technical field [0001] The invention relates to a method and application for rapidly enriching glucose-utilizing caproic acid-producing bacteria from pit mud, and belongs to the technical field of brewing microorganisms. Background technique [0002] Liquor is one of the six major distilled liquors in the world. During the long-term development process, a variety of flavored liquors have been formed. Among them, Luzhou-flavor liquor is deeply loved by consumers for its rich cellar aroma, soft and sweet taste, harmonious aroma, sweet entrance, soft mouth, and long tail. In the production of Luzhou-flavor liquor, pit mud is the basis for brewing Luzhou-flavor liquor, and the quality of pit mud determines the quality of liquor to a large extent. The physical and chemical tests and analysis of pit mud in recent years show that the pit mud with better quality has a grayish-gray sensory appearance and a strong ester aroma, has a near-neutral pH value (5.0-7.0), and a low lactic a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38C12G3/02C12R1/01
CPCC12N1/20C12N1/38C12G3/02
Inventor 任聪徐岩王慧琳
Owner JIANGNAN UNIV
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