Colloidal gold test strip for detecting HIV antibody in urine

Abstract

The invention discloses a colloidal gold test strip for detecting an HIV antibody in urine. The test strip comprises a plastic bottom plate, a sample pad, a nitrocellulose membrane, absorbent paper and a colloidal gold pad; the absorbent paper, the nitrocellulose membrane, the colloidal gold pad and the sample pad are all fixed on the plastic bottom plate; a colloidal gold labeled SPA and HIV gp41 fusion antigen is solidified on the colloidal gold pad; two ends of the nitrocellulose membrane are in lap joint with the inner end of the absorbent paper and the inner end of the colloidal gold pad respectively; the inner end of the sample pad is in lap joint with the outer end of the colloidal gold pad; the nitrocellulose membrane is provided with a detection line and a quality control line; the detection line is coated with an HIV gp41 recombinant antigen and a gp36 recombinant antigen in a mixed mode; and the quality control line is coated with a sheep polyclonal antibody. The test strip is used for preliminary screening of AIDS detection, is convenient and rapid to operate, realizes noninvasive sampling, has high detection sensitivity, and is suitable for self-detection of common people.

Description

Technical field[0001]The present invention relates to a test strip, and more particularly to a colloidal gold test strip that detects the HIV antibody in the urine.Background technique[0002]Human immunodeficiency virus is also known as HIV, which is a reverse transcription virus that causes human immune system defects. This virus will attack and gradually destroy the human immune system, causing the host to be protected when they are infected. Large-scale HIV detection is a severe challenge for human, material, financial, and time, especially with the global spread of AIDS, the risk examples of medical staff are infected with the development and innovation of HIV testing technology, "precise, fast Non-invasive "requirements.[0003]At present, the HIV diagnostic procedure is a first screen for positive, and Western blot is then confirmed. There are two main detection methods of the initial screen:[0004]1. Blood testing: mainly chemiluminescence, time-resolved fluorescence method, immu...

AI Technical Summary

Problems solved by technology

[0005] However, both chemiluminescence and time-resolved fluorescence in blood testing methods require large-scale equipment, which are only equipped in hospitals at the county level and above

Moreover, these two methods are time-consuming and require specialized technical operators

In addition, blood testing is a invasive test, and blood transmission is one of the three major routes of AIDS transmission. The shortcomings of blood testing methods are: (1) the risk of infection by medical staff during the blood drawing process; (2) ) There is a risk of infection in the waste and disposal of various related medical devices after blood drawing; (3) Patients cannot draw blood for testing by themselves to ensure personal privacy

The non/very weak infectious urine testing method is currently appro

Images