Traditional Chinese medicine composition for preventing and treating atherosclerosis

Abstract

The invention relates to a traditional Chinese medicine composition for preventing and treating atherosclerosis, which is prepared by the following steps: preparing a drug extract of Chinese herbal medicine Viola philippica and edible white vinegar, and diluting into different concentrations; and the working process comprises the following steps: (1) intervening with endothelial cells with 100 mu mol / l Hcy of a traditional Chinese medicine composition to establish an endothelial cell apoptosis model, and detecting that the concentration Hcy can cause endothelial cell apoptosis through cell viability staining and Western Blot, thereby laying a foundation for subsequent experiments; (2) diluting the traditional Chinese medicine composition into liquid medicines with different concentration gradients (0 mu g / ml, 10 mu g / ml, 50 mu g / ml, 100 mu g / ml, 250 mu g / ml, 500 mu g / ml, 1 mg / ml and 10 mg / ml), intervening with cells for 24 hours and 48 hours, and verifying the optimal concentration range and action time of the medicine on the cell protection effect; and (3) verifying that the medicine has prevention and treatment effects on endothelial cell injury, infecting the endothelial cells by the obtained traditional Chinese medicine composition liquid medicine and Hcy according to different sequences, and detecting the cell survival rate. The invention plays an important role in endothelial cell injury, and provides a theoretical basis and medication guidance for later prevention and treatment of atherosclerosis.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to a traditional Chinese medicine composition for preventing and treating atherosclerosis. Background technique [0002] Cardiovascular and cerebrovascular diseases are characterized by high morbidity, high prevalence and high mortality, which has brought a serious economic burden to the country and is also an important factor affecting the healthy life of Chinese residents. At the same time, with the increase of various risk factors, the age of onset of cardiovascular and cerebrovascular diseases also has a younger trend. Atherosclerosis (AS) is the pathological basis of vascular lesions in cardiovascular and cerebrovascular diseases. AS has many etiologies such as: intimal injury, lipid infiltration, oxidative stress, inflammatory response, and vascular smooth muscle cell proliferation. etc., while intimal injury is considered to be the initiating factor of AS. Vascular endothe...

AI Technical Summary

Problems solved by technology

With the in-depth study of PCI, it was found that stent implantation, especially drug-eluting stent implantation, reduced the incidence of restenosis, but with the emergence of intravascular ultrasound technology and optical coherence tomography technology, post-stent implantation The problem of lumen restenosis is once again intuitively exposed to everyone

At present, although the drug-coated stent can inhibit the proliferation and migration of vascular smooth muscle to a certain extent, it still cannot completely prevent the proliferation and migration of vascular smooth muscle, and inhibiting the regeneration and migration of vascular endothelial cells will lead to long-term exposure of the stent in the vascular lumen. In-stent restenosis and thrombosis are prone to occur. Therefore, finding effective drugs and technologies for the prevention and treatment of atherosclerosis has become one of the problems to be solved in the cardiovascular field.

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