Oligopeptide and active peptide composition and application of oligopeptide and active peptide composition in preparation of product with anti-inflammatory effect

An anti-inflammatory and active peptide technology, applied in the field of biomedicine, can solve the problems of chronic inflammation and cell damage in the skin, and achieve the effects of convenient operation, enhanced activity, and simple preparation process

Inactive Publication Date: 2021-06-04
深圳海创生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The immune response of the skin is crucial for the host's defense against pathogenic microorganisms, but immune dysregulation can lead to chronic inflammation in the skin, resulting in persistent cell damage and various skin diseases

Method used

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  • Oligopeptide and active peptide composition and application of oligopeptide and active peptide composition in preparation of product with anti-inflammatory effect
  • Oligopeptide and active peptide composition and application of oligopeptide and active peptide composition in preparation of product with anti-inflammatory effect
  • Oligopeptide and active peptide composition and application of oligopeptide and active peptide composition in preparation of product with anti-inflammatory effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 The separation of bifidobacterium oligopeptide-α and Saccharomyces cerevisiae cyclic peptide-β

[0034] Mix 50g of bifidobacteria with 500mL of ethyl acetate and 2000mL of water evenly, ultrasonicate for 20min, extract the water phase layer after standing, and repeat the extraction process several times, vacuum and freeze-dry the water phase layer, and then carry out preparative HPLC Prepare and isolate to obtain bifidobacterium oligopeptide-α (HT-1).

[0035] Mix 50g of brewer's yeast with 500mL of ethyl acetate and 2000mL of water, ultrasonically for 20min, extract the water phase layer after standing, repeat the extraction process several times, vacuum and freeze-dry the water phase layer, and then carry out preparative HPLC Prepare and isolate to obtain Saccharomyces cerevisiae cyclic peptide-β (DF-1).

[0036] The preparation conditions of the above-mentioned preparative HPLC are: 0.1% trifluoroacetic acid aqueous solution is used as mobile phase A, and ...

Embodiment 2

[0037] Example 2 Chemical Synthesis of Bifidobacterium Oligopeptide-α (HT-1) and Saccharomyces Saccharomyces Cyclopeptide-β (DF-1)

[0038](1) Take 100mg of Fmoc-Tyr Wang Resin and place it in a solid-phase synthesis tube, add N,N-dimethylformamide (DMF) and let it stand for 30 minutes to fully swell the resin, filter off the solvent, and then add piperidine DMF solution, and the solvent was filtered off after shaking. Dissolve Fmoc-Ser-OH, 1-hydroxybenzotriazole, O-benzotriazole-tetramethyluronium hexafluorophosphate in DMF, add N,N-diisopropylethylamine and mix well After being protected from light, it has been activated, and added to the resin, blown and stirred with nitrogen at 25°C for 2 hours, filtered with suction, washed with DMF and dichloromethane in sequence, and dried the solvent. Repeat the above steps, add the activated Fmoc-Leu-OH, Fmoc-His-OH, Fmoc-Leu-OH, Fmoc-His-OH, Fmoc-Gly-OH to the resin in sequence, blow and stir with nitrogen at 25°C, After the reacti...

experiment example

[0044] In order to evaluate the biological activity of the bifidobacterium oligopeptide-α (HT-1) and Saccharomyces cerevisiae cyclic peptide-β (DF-1) of the present invention and their combinations, the following effect examples were carried out.

[0045] The immortalized keratinocyte line HaCaT cells were cultured in a cell incubator at 37°C, 5% CO 2 (DMEM medium). The concentration of LPS was 1 μM to stimulate for 12 hours. After the end, the cells were washed with PBS, the PBS was discarded, and DMEM medium or medium containing HT-1 or DF-1 (20 μM) was added to continue culturing for 24 hours. The CCK8 method was used to detect cell viability, and the cells and culture fluid were collected.

[0046] In the test of the effect of HT-1 or DF-1 on the viability of HaCaT cells, the experiment was divided into 4 groups: normal control group (Control); LPS group; HT-1 20μM group (LPS+HT-1 20μM); DF-1 20 μM group (LPS+DF-1 20 μM). Each treatment condition had 3 replicate wells, ...

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Abstract

The invention relates to the technical field of biomedicine, and particularly discloses an oligopeptide and an active peptide composition and application of the oligopeptide and the active peptide composition in preparation of a product with an anti-inflammatory effect. The oligopeptide has a structure as shown in a formula I which is described in the specification. The active peptide composition comprises the oligopeptide with the structure as shown in the formula I and active cyclic peptide with the structure as shown in a formula II; and experiments show that both the oligopeptide bifidobacterium oligopeptide-alpha and the active peptide saccharomyces cerevisiae cyclic peptide-beta can inhibit HaCaT cell inflammatory injury and apoptosis caused by LPS exposure. Therefore, the bifidobacterium oligopeptide-alpha, the saccharomyces cerevisiae cyclopeptide-beta and the composition of the bifidobacterium oligopeptide-alpha and the saccharomyces cerevisiae cyclopeptide-beta can be used as active substances in cosmetics, skin care products, foods, health care products or medicines to play an anti-inflammatory role.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an oligopeptide, an active peptide composition and its application in preparing products with anti-inflammatory effects. Background technique [0002] Inflammation is an adaptive response induced by various external stimuli. It is one of the most basic protective responses of normal organisms and cells to foreign bodies such as pathogens and viruses. It is the basis of various physiological and pathological processes. , toxic or autoimmune damaged tissues. The inflammatory process is also the first step in the immune response to toxins, invading pathogens and allergens, and to damaged tissues. [0003] The skin is the largest protective organ of the human body. As the interface between the human body and the environment, the skin forms a strong barrier to resist damage from external stimuli. In inflammatory diseases with impaired barrier function such as atopic dermatitis, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/14A61K38/16A61K8/64A61P29/00A61Q19/00
CPCC07K7/06A61K38/164A61K8/64A61Q19/00A61P29/00
Inventor 刘志刚刘杰牛文芳王志尧刘晓宇韩艳霞
Owner 深圳海创生物科技有限公司
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