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A compound flora for preventing and controlling the outbreak of Fusarium spp. in maize and its application

A technology of complex flora and Fusarium, applied in the direction of application, chemicals for biological control, bacteria, etc., can solve the problems of endangering human and animal health, environmental pollution, etc., and achieve the effect of reducing the outbreak rate and reducing the number

Active Publication Date: 2022-05-20
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Conventional chemical prevention and control measures will cause environmental pollution, endanger the health of humans and animals, etc.

Method used

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  • A compound flora for preventing and controlling the outbreak of Fusarium spp. in maize and its application
  • A compound flora for preventing and controlling the outbreak of Fusarium spp. in maize and its application
  • A compound flora for preventing and controlling the outbreak of Fusarium spp. in maize and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Effect of Fusarium species outbreak on maize growth

[0049] The experiment set up two treatments, natural soil planting (NS) and sterile soil planting (SS), 150g of natural soil and sterilized soil were placed in sterile tissue culture bottles, 37.5ml of sterile water was added, stirred evenly, and cultivated in the dark for 1 week to ensure that the soil moisture content was 20%-25%. The sterilized soil was sterilized by γ rays, and the irradiation dose > 50 kGray.

[0050] Transplant the germinated maize seeds from sterile flat dish into tissue culture flasks with 5 maize planted in each flask and set up 6 replicates. The potted experimental corn planting conditions are 25 °C, light 16h dark 8h, humidity is 50%.

[0051]After 8 days of maize growth, the incidence of maize was counted, and the activities of plant height, fresh weight, chlorophyll SPAD value, SOD, POD and CAT enzyme were measured. Plant height and fresh weight are determined using tape measures ...

Embodiment 2

[0056] Example 2: Isolation and identification of healthy maize rhizosphere culturable strains

[0057] Potted corn planting conditions are the same as example 1.

[0058] Healthy maize plants in natural soil were selected, maize rhizosphere samples were collected on the 8th day after transplanting, and maize rhizosphere culturable strains were isolated by a combination of macro culture omics separation and plate coating.

[0059] Take the rhizosphere soil to prepare soil microbial extract, then perform gradient dilution, select 10 -4 and 10 -5Dilution of the microbial extract was performed in a 96-well plate with 1:10 (v / v) trypsin soy broth medium added. Only 30%-40% well-grown microtiter plates were selected for strain 16S rRNA gene identification, the bacterial fluid was extracted from genomic DNA, amplified by polymerase chain reaction (PCR) technique using two-step barcode labeling primers, 6 μL of bacterial culture was added to 10 μL buffer containing 25 mM NaOH and 0.2 mME...

Embodiment 3

[0062] Example 3: Antibacterial determination of the core antagonism in the complex flora

[0063] 8 strains were separately incubated on solid TSB medium and placed in a 30 °C incubator. After the formation of monobacteria, each strain was picked in 3 ml of liquid TSB medium and shook at 170 rpm overnight. Aspirate 2 ml of bacterial solution into a sterile centrifuge tube, centrifuge 5 seeds at 8000 rmp, and then resuspend with PBS buffer to obtain the suspension of the test strain.

[0064] Inoculate Maize Seed Habitat Fusarium into the center of the PDA plate and place in a 28 °C incubator. After the hyphae are laid out on the plate, use a sterile perforator to take the fungal fungal mass and transfer it to the center of the new PDA solid plate. Aspirate 10 μL of the test strain suspension at equal distance on both sides of the fungal mass and place in a 30 °C incubator for 8 days.

[0065] Results and analysis

[0066] as Figure 5As shown, in the complex flora, only Bacillus R...

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Abstract

The invention discloses a compound flora for preventing and controlling the outbreak of Fusarium spp. in maize and its application. The complex flora is composed of Acinetobacter baumannii RFAci-1, Pseudomonas stutzeri RFPse-1, Pseudomonas japonicus RFPth-1, Burkholderia cepacia RFBur-1, oligotrophic monocytotrophs maltophilia Bacillus RFSte‑1, Bacillus amyloliquefaciens RFBac‑1, Agrolysobacter RFLys‑1 and Enterobacter ludwig RFEnt‑1. The compound flora has a stable structure and has the characteristics of high-efficiency biocontrol and growth promotion. Inoculation into the soil can significantly reduce the incidence of Fusarium disease caused by Fusarium spp. in the main corn varieties in my country, and can also promote the growth of corn plants.

Description

Technical field [0001] The present invention relates to a kind of control of maize seed habitat Fusarium caused by disease rhizosphere composite flora and its application, belongs to the field of crop soil-borne disease control technology. Background [0002] The germ-dwelling microorganisms inside the seeds constitute a very rich microbial community, and they are not a single reciprocal symbiotic relationship with the plant, in which there are also pathogenic pathogenic microorganisms. Most seeds carry pathogenic fungi inside, and their dominant genera are Fusarium species, such as Fusarium aculis, Fusarium rotiferia, Fusarium laminate, Fusarium graminearum, etc. Fusarium fusarium is transmitted mainly through vertical transmission between plant species into the plant seed, and later becomes the source of infection in the second year, which in turn causes various plant diseases. [0003] Species Fusarium can produce toxins such as Deoxyfus Fusarium enol (DON) and Vormatoxin (FB1...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01N63/27A01N63/20A01P21/00A01P3/00A01G22/20C12R1/38C12R1/07C12R1/01
CPCC12N1/20A01N63/22A01N63/27A01N63/20A01G22/20
Inventor 荀卫兵张瑞福刘梓豪任轶张楠徐志辉沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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