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Antibacterial composition for in-vitro inhibition of pathogenic bacteria of visceral white spots of large yellow croakers, and preparation method thereof

A composition and technology for large yellow croaker, which are applied in the directions of antibacterial drugs, pharmaceutical formulations, peptide/protein components, etc., can solve the problems of the bacteriostatic composition inhibiting the visceral white spot pathogenic bacteria Pseudomonas of large yellow croaker, etc. Effectiveness in reducing outbreak rates of drug-resistant bacteria

Inactive Publication Date: 2020-03-24
FUJIAN MINDONG AQUATIC PROD RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, there is no method for inhibiting large yellow croaker viscera white spot pathogenic bacteria Pseudomonas by mixing Nisin-B and EDTA-2Na to prepare an antibacterial composition

Method used

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  • Antibacterial composition for in-vitro inhibition of pathogenic bacteria of visceral white spots of large yellow croakers, and preparation method thereof
  • Antibacterial composition for in-vitro inhibition of pathogenic bacteria of visceral white spots of large yellow croakers, and preparation method thereof

Examples

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Effect test

Embodiment 1

[0023] A method for preparing an antibacterial composition for inhibiting white spot pathogenic bacteria in large yellow croaker viscera in vitro, the steps of the preparation method for the antibacterial composition are as follows: S1. Extraction of nisin-B: take Lactobacillus lactis K4 producing nisin-B Carry out overnight culture in nutrient broth, take the overnight culture solution of Lactobacillus lactis and add it to nutrient broth, carry out constant temperature culture, centrifuge for the first time, take supernatant, add saturated ammonium sulfate, let it stand overnight, and centrifuge for the second time , take the precipitate and add it into sterile distilled water to obtain the crude extract of nisin-B; S2. Preparation of antibacterial composition: dissolve EDTA-2Na in sterile water, and then add the crude extract of nisin-B , and mix uniformly to prepare an antibacterial composition.

[0024] The ratio by weight of Lactobacillus lactis K4 and nutrient broth in S...

Embodiment 2

[0032] A method for preparing an antibacterial composition for inhibiting white spot pathogenic bacteria in large yellow croaker viscera in vitro, the steps of the preparation method for the antibacterial composition are as follows: S1. Extraction of nisin-B: take Lactobacillus lactis K4 producing nisin-B Carry out overnight culture in nutrient broth, take the overnight culture solution of Lactobacillus lactis and add it to nutrient broth, carry out constant temperature culture, centrifuge for the first time, take supernatant, add saturated ammonium sulfate, let it stand overnight, and centrifuge for the second time , take the precipitate and add it into sterile distilled water to obtain the crude extract of nisin-B; S2. Preparation of antibacterial composition: dissolve EDTA-2Na in sterile water, and then add the crude extract of nisin-B , and mix uniformly to prepare an antibacterial composition.

[0033] The ratio by weight of Lactobacillus lactis K4 and nutrient broth in S...

Embodiment 3

[0041] A method for preparing an antibacterial composition for inhibiting white spot pathogenic bacteria in large yellow croaker viscera in vitro, the steps of the preparation method for the antibacterial composition are as follows: S1. Extraction of nisin-B: take Lactobacillus lactis K4 producing nisin-B Carry out overnight culture in nutrient broth, take the overnight culture solution of Lactobacillus lactis and add it to nutrient broth, carry out constant temperature culture, centrifuge for the first time, take supernatant, add saturated ammonium sulfate, let it stand overnight, and centrifuge for the second time , take the precipitate and add it into sterile distilled water to obtain the crude extract of nisin-B; S2. Preparation of antibacterial composition: dissolve EDTA-2Na in sterile water, and then add the crude extract of nisin-B , and mix uniformly to prepare an antibacterial composition.

[0042] The ratio by weight of Lactobacillus lactis K4 and nutrient broth in S...

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Abstract

The invention discloses an antibacterial composition for in-vitro inhibition of pathogenic bacteria of visceral white spots of large yellow croakers, and a preparation method thereof, and relates to the technical field of antibacterial compositions. The preparation method of the antibacterial composition comprises the following steps: S1, extracting nisin-B: taking Lactobacillus lactis K4 capableof producing nisin-B, culturing the Lactobacillus lactis K4 in a nutritional broth overnight, taking the overnight cultured Lactobacillus lactis solution, adding the nutrient broth, carrying out constant-temperature culture, carrying out primary centrifugation, taking the obtained supernatant, adding saturated ammonium sulfate, standing the obtained solution overnight, carrying out secondary centrifugation, taking the obtained precipitate, and adding sterile distilled water so as to obtain crude nisin-B extract; and S2, preparing the antibacterial composition: dissolving EDTA-2Na in sterile water, then adding the crude nisin-B extract, and performing uniform mixing so as to obtain the antibacterial composition.

Description

technical field [0001] The invention relates to the technical field of antibacterial compositions, in particular to an antibacterial composition and a preparation method for inhibiting white spot pathogenic bacteria in viscera of large yellow croaker in vitro. Background technique [0002] The large yellow croaker has fresh and tender meat and high nutritional value. It is the main offshore economic fish in my country. Visceral white spot disease of large yellow croaker is one of the more common diseases of large yellow croaker cultured in cages in recent years. Related studies have shown that Pseudomonas spp. may be the main pathogenic bacteria causing visceral white spot disease of large yellow croaker. Generally, during the outbreak of white spot disease in the viscera of large yellow croaker, cage farmers mainly adopt the method of excessive administration of drugs to inhibit Pseudomonas. However, excessive use of drugs, especially antibiotics, can easily lead to the d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61P31/04
CPCA61K38/164A61P31/04
Inventor 徐春霞王伟黄惠珍张芳芳谢友佺迟海
Owner FUJIAN MINDONG AQUATIC PROD RES INST
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