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A kind of pangolin fingerprint quality monitoring method

A fingerprint, quality monitoring technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of limited existing quantity, low sensitivity of thin-layer analysis, immature artificial breeding technology, etc., and achieve the effect of simple analysis method

Active Publication Date: 2022-07-22
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese pangolin is a national second-class protected animal. The existing number is very limited, and the artificial breeding technology is not yet mature, resulting in a shortage of pangolin medicinal resources.
Under such circumstances, pangolins from similar animal sources such as Malayan pangolins, Indian pangolins, and tree pangolins of the same genus and different species are flooding the pangolin market [Liu Xun, Chinese Medicinal Materials, 2017, 40(3), 585-588] , and even trotter shells are used to adulterate pangolins [Shi Shangyou, Herald of Traditional Chinese Medicine, 2007, 13(11), 73], resulting in uneven quality of pangolins
[0004] The Pharmacopoeia stipulates that the identification methods of pangolins and their processed products include character identification and thin-layer chromatography analysis. The former is easily limited by the state of the decoction pieces and personnel experience, while the sensitivity of the thin-layer analysis method is low.

Method used

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  • A kind of pangolin fingerprint quality monitoring method
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  • A kind of pangolin fingerprint quality monitoring method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0030] 1. Analysis conditions:

[0031] Instrument: Waters Alliance

[0032] Chromatographic column: Waters Symmetry 300 C18 (4.6×150mm, 5μm, )

[0033] Flow rate: 1.0mL / min

[0034] Column temperature: 30℃

[0035] Injection volume: 10μL

[0036] Detection wavelength: 280nm

[0037] Mobile phase: A. 0.1% trifluoroacetic acid / water B. 0.1% trifluoroacetic acid / acetonitrile (volume concentration)

[0038] gradient:

[0039]

[0040]

[0041] 2. Preparation of the test solution: Take the pangolin sample C1, smash it into powder, pass it through a 45-mesh sieve, weigh 1 mg of the powder that has passed through the sieve, put it in a container, add the extractant according to the volume of 1 mg powder: 25 mL, and heat at 100 °C Reflux for 1 hour, let it cool, weigh again, use the extract to make up the lost weight, shake well, take the supernatant and pass it through a polyethersulfone filter membrane with a pore size of 0.45 μm, and take the filtrate.

[0042] 3. P...

Embodiment 2

[0044] The difference with Example 1 is that the flow rate is 0.9mL / min, and the remaining conditions are the same as those in Example 1, and the fingerprint spectrum is shown in figure 2 .

Embodiment 3

[0046] The difference from Example 1 is that the column temperature is 35°C, and other conditions are the same as those of Example 1, and the fingerprint spectrum is shown in Figure 3.

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Abstract

The invention relates to a method for monitoring the quality of pangolin fingerprints, belonging to the field of quality analysis of traditional Chinese medicines. The specific implementation is as follows: 1. Preparation of Chinese pangolin methyl source pangolin test solution; 2. Chromatographic analysis of Chinese pangolin methyl source pangolin test solution; Preparation, Analysis and Origin Identification. The invention can effectively realize the effective distinction of different base pangolins or fake pangolins, and provides a quick and simple detection method for the quality control of pangolins.

Description

technical field [0001] The invention relates to a method for monitoring the quality of pangolin fingerprints, belonging to the field of quality analysis of traditional Chinese medicines. [0002] technical background [0003] Pangolin is a kind of animal medicine with a long history of medicine. The commonly used clinical forms are cannon golin and vinegar goat extracted by sand scalding or sand scalding. They have the functions of promoting blood circulation and eliminating symptoms, clearing the milk, reducing swelling and discharging pus. For the treatment of milk obstruction, carbuncle sore, rheumatic arthralgia embolism. The 2015 edition of the Chinese Pharmacopoeia records its animal origin as the scales of the Chinese pangolin (Manispentadactyla Linnaeus) (Pangolin). The Chinese pangolin is a national second-class protected animal, and the existing number is very limited, and the artificial breeding technology is not mature, resulting in a shortage of pangolin medicin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/86G01N30/74
CPCG01N30/02G01N30/8686G01N30/74
Inventor 梁鑫淼乔亚丽沈爱金郭志谋徐青刘艳芳郭学东陈相银
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI