A kind of pangolin fingerprint quality monitoring method
A fingerprint, quality monitoring technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of limited existing quantity, low sensitivity of thin-layer analysis, immature artificial breeding technology, etc., and achieve the effect of simple analysis method
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[0030] 1. Analysis conditions:
[0031] Instrument: Waters Alliance
[0032] Chromatographic column: Waters Symmetry 300 C18 (4.6×150mm, 5μm, )
[0033] Flow rate: 1.0mL / min
[0034] Column temperature: 30℃
[0035] Injection volume: 10μL
[0036] Detection wavelength: 280nm
[0037] Mobile phase: A. 0.1% trifluoroacetic acid / water B. 0.1% trifluoroacetic acid / acetonitrile (volume concentration)
[0038] gradient:
[0039]
[0040]
[0041] 2. Preparation of the test solution: Take the pangolin sample C1, smash it into powder, pass it through a 45-mesh sieve, weigh 1 mg of the powder that has passed through the sieve, put it in a container, add the extractant according to the volume of 1 mg powder: 25 mL, and heat at 100 °C Reflux for 1 hour, let it cool, weigh again, use the extract to make up the lost weight, shake well, take the supernatant and pass it through a polyethersulfone filter membrane with a pore size of 0.45 μm, and take the filtrate.
[0042] 3. P...
Embodiment 2
[0044] The difference with Example 1 is that the flow rate is 0.9mL / min, and the remaining conditions are the same as those in Example 1, and the fingerprint spectrum is shown in figure 2 .
Embodiment 3
[0046] The difference from Example 1 is that the column temperature is 35°C, and other conditions are the same as those of Example 1, and the fingerprint spectrum is shown in Figure 3.
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