Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel disinfection method for marine aquaculture water body

A technology for mariculture and disinfection methods, applied in chemical instruments and methods, animal husbandry wastewater treatment, light water/sewage treatment, etc., can solve problems such as complex components and lack of research

Active Publication Date: 2021-06-11
GUANGXI UNIV
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems that need to be further explored and solved in this technology, such as the control of operating conditions in the disinfection process, the inactivation mechanism of microorganisms, efficient and economical SO 4 - Selection of activation method, etc.
Also, on SO 4 - There are no reports on the application research in water disinfection before stocking seedlings in recirculating mariculture and the inactivation mechanism of various pathogenic microorganisms in the water.
Moreover, the composition of mariculture water is much more complex than that of fresh water, and there are a large number of various anions in it. The influence of different physical and chemical conditions on various pathogenic microorganisms, and the influence of the same pathogenic microorganism by different physical and chemical conditions are also lacking.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel disinfection method for marine aquaculture water body
  • Novel disinfection method for marine aquaculture water body
  • Novel disinfection method for marine aquaculture water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] After thawing the frozen Streptococcus agalactiae species preserved in the refrigerator at -80°C, they were inoculated in the BHI solid nutrient broth medium, and activated at 37°C. After a single colony grows well, use an inoculation loop to pick and inoculate it in 5mL BHI liquid nutrient broth medium, shake and culture at 37°C and a rotation speed of 300r / min until the logarithmic growth phase, take out 1mL of the bacterial liquid to Centrifuge at a speed of 4000r / min for 5 minutes, then resuspend the precipitate in sterilized saline, repeat the operation twice, and dissolve the precipitated bacterial mass in 100mL sterilized marine culture water to prepare 10 7 CFU / mL suspension.

Embodiment 2

[0026] Formulated with Cl - 、HCO 3 - , SO 4 2- 、Br - Concentrations of 10.489g / L, 65.5mg / L, 1.35g / L, 27.9mg / L artificial mariculture water to simulate real mariculture water, without adjusting the pH value of mariculture water. Prepare sodium persulfate solutions of 0.05mmol / L, 0.10mmol / L, 0.15mmol / L, 0.20mmol / L, 0.25mmol / L, and 0.30mmol / L respectively, and fix the UV intensity at 30uw / cm 2 After turning on the 10W low-pressure mercury lamp and stabilizing it for a period of time, the prepared 10 7 CFU / mL Streptococcus agalactiae suspension was aseptically pipetted 30mL into a translucent quartz reaction tube, put a magnetite, added 300uL sodium persulfate solutions of different concentrations, and put the quartz reaction tube into In a UV-stabilized reactor, take out the reaction tube for sampling at different reaction times, dilute the sample in a sterile ultra-clean workbench, streak and coat the corresponding solid medium, and place it in a 37°C incubator for upside-...

Embodiment 3

[0029] Prepare artificial mariculture water identically with example 2, prepare the sodium persulfate solution that concentration is 0.1mmol / L, regulate the artificial mariculture water that pH value is 5, 6, 7, 8, 9 respectively, fix at 30uw / cm 2 After turning on the 10W low-pressure mercury lamp and stabilizing for a period of time, all the other reaction steps are the same as in Example 2, and the inactivation rate of Streptococcus agalactiae in the artificial mariculture water of different pH values ​​by UV / PS is calculated.

[0030] Such as figure 2 As shown, the experiment found that when the reaction time was the same, the inactivation efficiency of Streptococcus agalactiae was the highest when the pH was 5, and the inactivation efficiency was the lowest when the pH was 9. With the change of pH from 9 to 5, UV The inactivation effect of / PS on Streptococcus agalactiae gradually increased. SO in the system under acidic and neutral conditions 4 - Dominant, SO 4 - ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a novel disinfection method for a marine aquaculture water body. The marine aquaculture water body containing streptococcus agalactiae is disinfected and inactivated by using a UV / PS advanced oxidation method. According to the novel disinfection method for the marine aquaculture water body, gram-positive bacteria, namely, S.agalactiae, in the marine aquaculture water body can be effectively inactivated, and toxic and harmful substances and secondary pollution are not generated in the disinfection treatment process. In conclusion, the method for disinfecting the marine aquaculture water has unique advantages, the preparation technology is simple, the cost is low, the microorganism killing speed is extremely high, and the method is efficient and reliable.

Description

technical field [0001] The invention belongs to the technical field of water pollution disinfection, and in particular relates to a novel method for disinfection of mariculture water. Background technique [0002] Mariculture is a traditional advantageous industry in my country's coastal areas. It not only drives the economic and social development of fishing areas, but also meets the needs of urban and rural residents with diversified dietary nutrition structures. According to statistics, the total output of mariculture in China reached 20.007 million tons in 2017. The recirculating marine aquaculture system treats and recycles the used water after harvesting each season's products, which reduces the consumption of seawater and freshwater resources, and has been widely used. In circulating seawater aquaculture ponds, seawater will bring in various pathogenic bacteria; on the other hand, after stocking fish and shrimp, after a period of cultivation, the accumulation of fish...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/50C02F1/32C02F103/20
CPCC02F1/50C02F1/32C02F2103/20C02F2303/04
Inventor 张媛媛韦敏黄坤玲朱云杰余克服
Owner GUANGXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com